Skip to main content
Journal of Clinical Microbiology logoLink to Journal of Clinical Microbiology
. 1994 Aug;32(8):2007–2008. doi: 10.1128/jcm.32.8.2007-2008.1994

Identification and elimination of DNA sequences in Taq DNA polymerase.

M S Hughes 1, L A Beck 1, R A Skuce 1
PMCID: PMC263920  PMID: 7989558

Abstract

This study confirms that different preparations of Taq DNA polymerase are contaminated with eubacterial DNA. The contaminants appeared to represent more than one strain or species but were not identified as Thermus aquaticus or Escherichia coli. Differences in microcentrifuge tube composition appeared to affect elimination of the contaminants.

Full text

PDF
2007

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Böttger E. C. Frequent contamination of Taq polymerase with DNA. Clin Chem. 1990 Jun;36(6):1258–1259. [PubMed] [Google Scholar]
  2. Hughes M. S., Skuce R. A., Beck L. A., Neill S. D. Identification of mycobacteria from animals by restriction enzyme analysis and direct DNA cycle sequencing of polymerase chain reaction-amplified 16S rRNA gene sequences. J Clin Microbiol. 1993 Dec;31(12):3216–3222. doi: 10.1128/jcm.31.12.3216-3222.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Meier A., Persing D. H., Finken M., Böttger E. C. Elimination of contaminating DNA within polymerase chain reaction reagents: implications for a general approach to detection of uncultured pathogens. J Clin Microbiol. 1993 Mar;31(3):646–652. doi: 10.1128/jcm.31.3.646-652.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Rand K. H., Houck H. Taq polymerase contains bacterial DNA of unknown origin. Mol Cell Probes. 1990 Dec;4(6):445–450. doi: 10.1016/0890-8508(90)90003-i. [DOI] [PubMed] [Google Scholar]
  5. Schmidt T. M., Pace B., Pace N. R. Detection of DNA contamination in Taq polymerase. Biotechniques. 1991 Aug;11(2):176–177. [PubMed] [Google Scholar]

Articles from Journal of Clinical Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES