Abstract
This study confirms that different preparations of Taq DNA polymerase are contaminated with eubacterial DNA. The contaminants appeared to represent more than one strain or species but were not identified as Thermus aquaticus or Escherichia coli. Differences in microcentrifuge tube composition appeared to affect elimination of the contaminants.
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- Böttger E. C. Frequent contamination of Taq polymerase with DNA. Clin Chem. 1990 Jun;36(6):1258–1259. [PubMed] [Google Scholar]
- Hughes M. S., Skuce R. A., Beck L. A., Neill S. D. Identification of mycobacteria from animals by restriction enzyme analysis and direct DNA cycle sequencing of polymerase chain reaction-amplified 16S rRNA gene sequences. J Clin Microbiol. 1993 Dec;31(12):3216–3222. doi: 10.1128/jcm.31.12.3216-3222.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Meier A., Persing D. H., Finken M., Böttger E. C. Elimination of contaminating DNA within polymerase chain reaction reagents: implications for a general approach to detection of uncultured pathogens. J Clin Microbiol. 1993 Mar;31(3):646–652. doi: 10.1128/jcm.31.3.646-652.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Rand K. H., Houck H. Taq polymerase contains bacterial DNA of unknown origin. Mol Cell Probes. 1990 Dec;4(6):445–450. doi: 10.1016/0890-8508(90)90003-i. [DOI] [PubMed] [Google Scholar]
- Schmidt T. M., Pace B., Pace N. R. Detection of DNA contamination in Taq polymerase. Biotechniques. 1991 Aug;11(2):176–177. [PubMed] [Google Scholar]