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. 1994 Aug;32(8):2031–2034. doi: 10.1128/jcm.32.8.2031-2034.1994

Genotyping of Canadian hepatitis C virus isolates by PCR.

A Andonov 1, R K Chaudhary 1
PMCID: PMC263927  PMID: 7989565

Abstract

We used PCR for hepatitis C virus (HCV) genotyping with type-specific primers from the core and NS5 genes. Type I was predominant in the general population (58% in blood donors) as well as in different risk groups, such as intravenous drug abusers (58%), blood transfusion recipients (64%), hemophiliacs (62%), and patients with HCV chronic liver disease (76%). Types II, III, and IV were less prevalent in Canada, being found in 10.92, 6.72, and 5.88% of the population, respectively. The type II core primer was not type specific and reacted with the majority of our type I HCV samples, suggesting a false-positive dual infection with two different genotypes (I and II). Digestion of these amplified type I and type II products with restriction endonuclease AccI proved to be very useful in the exclusion of false-positive dual type I and type II infections.

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Selected References

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  1. Bréchot C., Kremsdorf D. Genetic variation of the hepatitis C virus (HCV) genome: random events or a clinically relevant issue? J Hepatol. 1993 Mar;17(3):265–268. doi: 10.1016/s0168-8278(05)80203-x. [DOI] [PubMed] [Google Scholar]
  2. Bukh J., Purcell R. H., Miller R. H. At least 12 genotypes of hepatitis C virus predicted by sequence analysis of the putative E1 gene of isolates collected worldwide. Proc Natl Acad Sci U S A. 1993 Sep 1;90(17):8234–8238. doi: 10.1073/pnas.90.17.8234. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Chan S. W., McOmish F., Holmes E. C., Dow B., Peutherer J. F., Follett E., Yap P. L., Simmonds P. Analysis of a new hepatitis C virus type and its phylogenetic relationship to existing variants. J Gen Virol. 1992 May;73(Pt 5):1131–1141. doi: 10.1099/0022-1317-73-5-1131. [DOI] [PubMed] [Google Scholar]
  4. Chaudhary R. K., Andonov A., MacLean C. Detection of hepatitis C virus infection with recombinant immunoblot assay, synthetic immunoblot assay, and polymerase chain reaction. J Clin Lab Anal. 1993;7(3):164–167. doi: 10.1002/jcla.1860070306. [DOI] [PubMed] [Google Scholar]
  5. Chayama K., Tsubota A., Arase Y., Saitoh S., Koida I., Ikeda K., Matsumoto T., Kobayashi M., Iwasaki S., Koyama S. Genotypic subtyping of hepatitis C virus. J Gastroenterol Hepatol. 1993 Mar-Apr;8(2):150–156. doi: 10.1111/j.1440-1746.1993.tb01507.x. [DOI] [PubMed] [Google Scholar]
  6. Chomczynski P., Sacchi N. Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal Biochem. 1987 Apr;162(1):156–159. doi: 10.1006/abio.1987.9999. [DOI] [PubMed] [Google Scholar]
  7. Kanai K., Kako M., Okamoto H. HCV genotypes in chronic hepatitis C and response to interferon. Lancet. 1992 Jun 20;339(8808):1543–1543. doi: 10.1016/0140-6736(92)91311-u. [DOI] [PubMed] [Google Scholar]
  8. McOmish F., Chan S. W., Dow B. C., Gillon J., Frame W. D., Crawford R. J., Yap P. L., Follett E. A., Simmonds P. Detection of three types of hepatitis C virus in blood donors: investigation of type-specific differences in serologic reactivity and rate of alanine aminotransferase abnormalities. Transfusion. 1993 Jan;33(1):7–13. doi: 10.1046/j.1537-2995.1993.33193142314.x. [DOI] [PubMed] [Google Scholar]
  9. Müller H. M., Pfaff E., Goeser T., Theilmann L. Genetic variability of German hepatitis C virus isolates. J Med Virol. 1993 Aug;40(4):291–306. doi: 10.1002/jmv.1890400407. [DOI] [PubMed] [Google Scholar]
  10. Okamoto H., Kurai K., Okada S., Yamamoto K., Lizuka H., Tanaka T., Fukuda S., Tsuda F., Mishiro S. Full-length sequence of a hepatitis C virus genome having poor homology to reported isolates: comparative study of four distinct genotypes. Virology. 1992 May;188(1):331–341. doi: 10.1016/0042-6822(92)90762-e. [DOI] [PubMed] [Google Scholar]
  11. Okamoto H., Sugiyama Y., Okada S., Kurai K., Akahane Y., Sugai Y., Tanaka T., Sato K., Tsuda F., Miyakawa Y. Typing hepatitis C virus by polymerase chain reaction with type-specific primers: application to clinical surveys and tracing infectious sources. J Gen Virol. 1992 Mar;73(Pt 3):673–679. doi: 10.1099/0022-1317-73-3-673. [DOI] [PubMed] [Google Scholar]
  12. Okamoto H., Tokita H., Sakamoto M., Horikita M., Kojima M., Iizuka H., Mishiro S. Characterization of the genomic sequence of type V (or 3a) hepatitis C virus isolates and PCR primers for specific detection. J Gen Virol. 1993 Nov;74(Pt 11):2385–2390. doi: 10.1099/0022-1317-74-11-2385. [DOI] [PubMed] [Google Scholar]
  13. Pozzato G., Moretti M., Franzin F., Crocè L. S., Tiribelli C., Masayu T., Kaneko S., Unoura M., Kobayashi K. Severity of liver disease with different hepatitis C viral clones. Lancet. 1991 Aug 24;338(8765):509–509. doi: 10.1016/0140-6736(91)90578-d. [DOI] [PubMed] [Google Scholar]
  14. Simmonds P., Holmes E. C., Cha T. A., Chan S. W., McOmish F., Irvine B., Beall E., Yap P. L., Kolberg J., Urdea M. S. Classification of hepatitis C virus into six major genotypes and a series of subtypes by phylogenetic analysis of the NS-5 region. J Gen Virol. 1993 Nov;74(Pt 11):2391–2399. doi: 10.1099/0022-1317-74-11-2391. [DOI] [PubMed] [Google Scholar]
  15. Simmonds P., Rose K. A., Graham S., Chan S. W., McOmish F., Dow B. C., Follett E. A., Yap P. L., Marsden H. Mapping of serotype-specific, immunodominant epitopes in the NS-4 region of hepatitis C virus (HCV): use of type-specific peptides to serologically differentiate infections with HCV types 1, 2, and 3. J Clin Microbiol. 1993 Jun;31(6):1493–1503. doi: 10.1128/jcm.31.6.1493-1503.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Takada N., Takase S., Takada A., Date T. Differences in the hepatitis C virus genotypes in different countries. J Hepatol. 1993 Mar;17(3):277–283. doi: 10.1016/s0168-8278(05)80205-3. [DOI] [PubMed] [Google Scholar]
  17. Wang Y., Okamoto H., Tsuda F., Nagayama R., Tao Q. M., Mishiro S. Prevalence, genotypes, and an isolate (HC-C2) of hepatitis C virus in Chinese patients with liver disease. J Med Virol. 1993 Jul;40(3):254–260. doi: 10.1002/jmv.1890400316. [DOI] [PubMed] [Google Scholar]

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