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. 1997 Oct-Dec;3(4):529–539. doi: 10.3201/eid0304.970418

Epidemiology and detection as options for control of viral and parasitic foodborne disease.

L A Jaykus 1
PMCID: PMC2640072  PMID: 9366607

Abstract

Human enteric viruses and protozoal parasites are important causes of emerging food and waterborne disease. Epidemiologic investigation and detection of the agents in clinical, food, and water specimens, which are traditionally used to establish the cause of disease outbreaks, are either cumbersome, expensive, and frequently unavailable or unattempted for the important food and waterborne enteric viruses and protozoa. However, the recent introduction of regulatory testing mandates, alternative testing strategies, and increased epidemiologic surveillance for food and waterborne disease should significantly improve the ability to detect and control these agents. We discuss new methods of investigating foodborne viral and parasitic disease and the future of these methods in recognizing, identifying, and controlling disease agents.

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Selected References

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  1. Abbaszadegan M., Gerba C. P., Rose J. B. Detection of Giardia cysts with a cDNA probe and applications to water samples. Appl Environ Microbiol. 1991 Apr;57(4):927–931. doi: 10.1128/aem.57.4.927-931.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Ando T., Monroe S. S., Gentsch J. R., Jin Q., Lewis D. C., Glass R. I. Detection and differentiation of antigenically distinct small round-structured viruses (Norwalk-like viruses) by reverse transcription-PCR and southern hybridization. J Clin Microbiol. 1995 Jan;33(1):64–71. doi: 10.1128/jcm.33.1.64-71.1995. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Atmar R. L., Metcalf T. G., Neill F. H., Estes M. K. Detection of enteric viruses in oysters by using the polymerase chain reaction. Appl Environ Microbiol. 1993 Feb;59(2):631–635. doi: 10.1128/aem.59.2.631-635.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Atmar R. L., Neill F. H., Romalde J. L., Le Guyader F., Woodley C. M., Metcalf T. G., Estes M. K. Detection of Norwalk virus and hepatitis A virus in shellfish tissues with the PCR. Appl Environ Microbiol. 1995 Aug;61(8):3014–3018. doi: 10.1128/aem.61.8.3014-3018.1995. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Bean N. H., Goulding J. S., Lao C., Angulo F. J. Surveillance for foodborne-disease outbreaks--United States, 1988-1992. MMWR CDC Surveill Summ. 1996 Oct 25;45(5):1–66. [PubMed] [Google Scholar]
  6. Bean N. H., Griffin P. M., Goulding J. S., Ivey C. B. Foodborne disease outbreaks, 5-year summary, 1983-1987. MMWR CDC Surveill Summ. 1990 Mar;39(1):15–57. [PubMed] [Google Scholar]
  7. Campbell A. T., Robertson L. J., Smith H. V. Viability of Cryptosporidium parvum oocysts: correlation of in vitro excystation with inclusion or exclusion of fluorogenic vital dyes. Appl Environ Microbiol. 1992 Nov;58(11):3488–3493. doi: 10.1128/aem.58.11.3488-3493.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Campbell P. N., Current W. L. Demonstration of serum antibodies to Cryptosporidium sp. in normal and immunodeficient humans with confirmed infections. J Clin Microbiol. 1983 Jul;18(1):165–169. doi: 10.1128/jcm.18.1.165-169.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Cannon R. O., Poliner J. R., Hirschhorn R. B., Rodeheaver D. C., Silverman P. R., Brown E. A., Talbot G. H., Stine S. E., Monroe S. S., Dennis D. T. A multistate outbreak of Norwalk virus gastroenteritis associated with consumption of commercial ice. J Infect Dis. 1991 Nov;164(5):860–863. doi: 10.1093/infdis/164.5.860. [DOI] [PubMed] [Google Scholar]
  10. Centers for Disease Control and Prevention (CDC) Multistate outbreak of viral gastroenteritis related to consumption of oysters--Louisiana, Maryland, Mississippi and North Carolina, 1993. MMWR Morb Mortal Wkly Rep. 1993 Dec 17;42(49):945–948. [PubMed] [Google Scholar]
  11. Centers for Disease Control and Prevention (CDC) Viral gastroenteritis associated with consumption of raw oysters--Florida, 1993. MMWR Morb Mortal Wkly Rep. 1994 Jun 24;43(24):446–449. [PubMed] [Google Scholar]
  12. Chung H., Jaykus L. A., Sobsey M. D. Detection of human enteric viruses in oysters by in vivo and in vitro amplification of nucleic acids. Appl Environ Microbiol. 1996 Oct;62(10):3772–3778. doi: 10.1128/aem.62.10.3772-3778.1996. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Deng M. Y., Day S. P., Cliver D. O. Detection of hepatitis A virus in environmental samples by antigen-capture PCR. Appl Environ Microbiol. 1994 Jun;60(6):1927–1933. doi: 10.1128/aem.60.6.1927-1933.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Desenclos J. C., Klontz K. C., Wilder M. H., Nainan O. V., Margolis H. S., Gunn R. A. A multistate outbreak of hepatitis A caused by the consumption of raw oysters. Am J Public Health. 1991 Oct;81(10):1268–1272. doi: 10.2105/ajph.81.10.1268. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. DuPont H. L., Chappell C. L., Sterling C. R., Okhuysen P. C., Rose J. B., Jakubowski W. The infectivity of Cryptosporidium parvum in healthy volunteers. N Engl J Med. 1995 Mar 30;332(13):855–859. doi: 10.1056/NEJM199503303321304. [DOI] [PubMed] [Google Scholar]
  16. Goswami B. B., Koch W. H., Cebula T. A. Detection of hepatitis A virus in Mercenaria mercenaria by coupled reverse transcription and polymerase chain reaction. Appl Environ Microbiol. 1993 Sep;59(9):2765–2770. doi: 10.1128/aem.59.9.2765-2770.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Gouvea V., Santos N., Timenetsky M. do C., Estes M. K. Identification of Norwalk virus in artificially seeded shellfish and selected foods. J Virol Methods. 1994 Jul;48(2-3):177–187. doi: 10.1016/0166-0934(94)90117-1. [DOI] [PubMed] [Google Scholar]
  18. Green J., Norcott J. P., Lewis D., Arnold C., Brown D. W. Norwalk-like viruses: demonstration of genomic diversity by polymerase chain reaction. J Clin Microbiol. 1993 Nov;31(11):3007–3012. doi: 10.1128/jcm.31.11.3007-3012.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Haas C. N. Estimation of risk due to low doses of microorganisms: a comparison of alternative methodologies. Am J Epidemiol. 1983 Oct;118(4):573–582. doi: 10.1093/oxfordjournals.aje.a113662. [DOI] [PubMed] [Google Scholar]
  20. Hayes E. B., Matte T. D., O'Brien T. R., McKinley T. W., Logsdon G. S., Rose J. B., Ungar B. L., Word D. M., Pinsky P. F., Cummings M. L. Large community outbreak of cryptosporidiosis due to contamination of a filtered public water supply. N Engl J Med. 1989 May 25;320(21):1372–1376. doi: 10.1056/NEJM198905253202103. [DOI] [PubMed] [Google Scholar]
  21. Hedberg C. W., Osterholm M. T. Outbreaks of food-borne and waterborne viral gastroenteritis. Clin Microbiol Rev. 1993 Jul;6(3):199–210. doi: 10.1128/cmr.6.3.199. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Heun E. M., Vogt R. L., Hudson P. J., Parren S., Gary G. W. Risk factors for secondary transmission in households after a common-source outbreak of Norwalk gastroenteritis. Am J Epidemiol. 1987 Dec;126(6):1181–1186. doi: 10.1093/oxfordjournals.aje.a114756. [DOI] [PubMed] [Google Scholar]
  23. Jaykus L. A., De Leon R., Sobsey M. D. A virion concentration method for detection of human enteric viruses in oysters by PCR and oligoprobe hybridization. Appl Environ Microbiol. 1996 Jun;62(6):2074–2080. doi: 10.1128/aem.62.6.2074-2080.1996. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Jenkins S., Horman J. T., Israel E., Cukor G., Blacklow N. R. An outbreak of Norwalk-related gastroenteritis at a boys' camp. Am J Dis Child. 1985 Aug;139(8):787–789. doi: 10.1001/archpedi.1985.02140100049026. [DOI] [PubMed] [Google Scholar]
  25. Kaplan J. E., Feldman R., Campbell D. S., Lookabaugh C., Gary G. W. The frequency of a Norwalk-like pattern of illness in outbreaks of acute gastroenteritis. Am J Public Health. 1982 Dec;72(12):1329–1332. doi: 10.2105/ajph.72.12.1329. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Kaplan J. E., Gary G. W., Baron R. C., Singh N., Schonberger L. B., Feldman R., Greenberg H. B. Epidemiology of Norwalk gastroenteritis and the role of Norwalk virus in outbreaks of acute nonbacterial gastroenteritis. Ann Intern Med. 1982 Jun;96(6 Pt 1):756–761. doi: 10.7326/0003-4819-96-6-756. [DOI] [PubMed] [Google Scholar]
  27. Lambden P. R., Caul E. O., Ashley C. R., Clarke I. N. Sequence and genome organization of a human small round-structured (Norwalk-like) virus. Science. 1993 Jan 22;259(5094):516–519. doi: 10.1126/science.8380940. [DOI] [PubMed] [Google Scholar]
  28. LeChevallier M. W., Norton W. D., Lee R. G. Giardia and Cryptosporidium spp. in filtered drinking water supplies. Appl Environ Microbiol. 1991 Sep;57(9):2617–2621. doi: 10.1128/aem.57.9.2617-2621.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. LeChevallier M. W., Norton W. D., Siegel J. E., Abbaszadegan M. Evaluation of the immunofluorescence procedure for detection of Giardia cysts and Cryptosporidium oocysts in water. Appl Environ Microbiol. 1995 Feb;61(2):690–697. doi: 10.1128/aem.61.2.690-697.1995. [DOI] [PMC free article] [PubMed] [Google Scholar]
  30. Lees D. N., Henshilwood K., Doré W. J. Development of a method for detection of enteroviruses in shellfish by PCR with poliovirus as a model. Appl Environ Microbiol. 1994 Aug;60(8):2999–3005. doi: 10.1128/aem.60.8.2999-3005.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
  31. Lees D. N., Henshilwood K., Green J., Gallimore C. I., Brown D. W. Detection of small round structured viruses in shellfish by reverse transcription-PCR. Appl Environ Microbiol. 1995 Dec;61(12):4418–4424. doi: 10.1128/aem.61.12.4418-4424.1995. [DOI] [PMC free article] [PubMed] [Google Scholar]
  32. Mac Kenzie W. R., Hoxie N. J., Proctor M. E., Gradus M. S., Blair K. A., Peterson D. E., Kazmierczak J. J., Addiss D. G., Fox K. R., Rose J. B. A massive outbreak in Milwaukee of cryptosporidium infection transmitted through the public water supply. N Engl J Med. 1994 Jul 21;331(3):161–167. doi: 10.1056/NEJM199407213310304. [DOI] [PubMed] [Google Scholar]
  33. Mahbubani M. H., Bej A. K., Perlin M. H., Schaefer F. W., 3rd, Jakubowski W., Atlas R. M. Differentiation of Giardia duodenalis from other Giardia spp. by using polymerase chain reaction and gene probes. J Clin Microbiol. 1992 Jan;30(1):74–78. doi: 10.1128/jcm.30.1.74-78.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  34. Moe C. L., Gentsch J., Ando T., Grohmann G., Monroe S. S., Jiang X., Wang J., Estes M. K., Seto Y., Humphrey C. Application of PCR to detect Norwalk virus in fecal specimens from outbreaks of gastroenteritis. J Clin Microbiol. 1994 Mar;32(3):642–648. doi: 10.1128/jcm.32.3.642-648.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
  35. Nieminski E. C., Schaefer F. W., 3rd, Ongerth J. E. Comparison of two methods for detection of Giardia cysts and Cryptosporidium oocysts in water. Appl Environ Microbiol. 1995 May;61(5):1714–1719. doi: 10.1128/aem.61.5.1714-1719.1995. [DOI] [PMC free article] [PubMed] [Google Scholar]
  36. Ortega Y. R., Sterling C. R., Gilman R. H., Cama V. A., Díaz F. Cyclospora species--a new protozoan pathogen of humans. N Engl J Med. 1993 May 6;328(18):1308–1312. doi: 10.1056/NEJM199305063281804. [DOI] [PubMed] [Google Scholar]
  37. Rodgers M. R., Flanigan D. J., Jakubowski W. Identification of algae which interfere with the detection of Giardia cysts and Cryptosporidium oocysts and a method for alleviating this interference. Appl Environ Microbiol. 1995 Oct;61(10):3759–3763. doi: 10.1128/aem.61.10.3759-3763.1995. [DOI] [PMC free article] [PubMed] [Google Scholar]
  38. Sauch J. F., Flanigan D., Galvin M. L., Berman D., Jakubowski W. Propidium iodide as an indicator of Giardia cyst viability. Appl Environ Microbiol. 1991 Nov;57(11):3243–3247. doi: 10.1128/aem.57.11.3243-3247.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  39. Schupp D. G., Erlandsen S. L. A new method to determine Giardia cyst viability: correlation of fluorescein diacetate and propidium iodide staining with animal infectivity. Appl Environ Microbiol. 1987 Apr;53(4):704–707. doi: 10.1128/aem.53.4.704-707.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  40. Sobsey M. D., Carrick R. J., Jensen H. R. Improved methods for detecting enteric viruses in oysters. Appl Environ Microbiol. 1978 Jul;36(1):121–128. doi: 10.1128/aem.36.1.121-128.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  41. Ungar B. L., Soave R., Fayer R., Nash T. E. Enzyme immunoassay detection of immunoglobulin M and G antibodies to Cryptosporidium in immunocompetent and immunocompromised persons. J Infect Dis. 1986 Mar;153(3):570–578. doi: 10.1093/infdis/153.3.570. [DOI] [PubMed] [Google Scholar]
  42. Vesey G., Slade J. S., Byrne M., Shepherd K., Fricker C. R. A new method for the concentration of Cryptosporidium oocysts from water. J Appl Bacteriol. 1993 Jul;75(1):82–86. doi: 10.1111/j.1365-2672.1993.tb03412.x. [DOI] [PubMed] [Google Scholar]
  43. Wang J., Jiang X., Madore H. P., Gray J., Desselberger U., Ando T., Seto Y., Oishi I., Lew J. F., Green K. Y. Sequence diversity of small, round-structured viruses in the Norwalk virus group. J Virol. 1994 Sep;68(9):5982–5990. doi: 10.1128/jvi.68.9.5982-5990.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
  44. Warner R. D., Carr R. W., McCleskey F. K., Johnson P. C., Elmer L. M., Davison V. E. A large nontypical outbreak of Norwalk virus. Gastroenteritis associated with exposing celery to nonpotable water and with Citrobacter freundii. Arch Intern Med. 1991 Dec;151(12):2419–2424. doi: 10.1001/archinte.151.12.2419. [DOI] [PubMed] [Google Scholar]
  45. Webster K. A., Pow J. D., Giles M., Catchpole J., Woodward M. J. Detection of Cryptosporidium parvum using a specific polymerase chain reaction. Vet Parasitol. 1993 Oct;50(1-2):35–44. doi: 10.1016/0304-4017(93)90005-8. [DOI] [PubMed] [Google Scholar]
  46. White K. E., Osterholm M. T., Mariotti J. A., Korlath J. A., Lawrence D. H., Ristinen T. L., Greenberg H. B. A foodborne outbreak of Norwalk virus gastroenteritis. Evidence for post-recovery transmission. Am J Epidemiol. 1986 Jul;124(1):120–126. doi: 10.1093/oxfordjournals.aje.a114356. [DOI] [PubMed] [Google Scholar]
  47. Xi J. N., Graham D. Y., Wang K. N., Estes M. K. Norwalk virus genome cloning and characterization. Science. 1990 Dec 14;250(4987):1580–1583. doi: 10.1126/science.2177224. [DOI] [PubMed] [Google Scholar]

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