Abstract
A commercial kit (EnviroAmp) designed to detect the DNA of Legionella species in environmental water samples using PCR and reverse dot hybridization was applied to clinical specimens. Results correlated well with culture for bronchoalveolar lavages. In addition, this test was easy to perform and showed good sensitivity.
Full text
PDFSelected References
These references are in PubMed. This may not be the complete list of references from this article.
- Bangsborg J. M., Cianciotto N. P., Hindersson P. Nucleotide sequence analysis of the Legionella micdadei mip gene, encoding a 30-kilodalton analog of the Legionella pneumophila Mip protein. Infect Immun. 1991 Oct;59(10):3836–3840. doi: 10.1128/iai.59.10.3836-3840.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Bej A. K., Mahbubani M. H., Atlas R. M. Detection of viable Legionella pneumophila in water by polymerase chain reaction and gene probe methods. Appl Environ Microbiol. 1991 Feb;57(2):597–600. doi: 10.1128/aem.57.2.597-600.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Brenner D. J., Steigerwalt A. G., McDade J. E. Classification of the Legionnaires' disease bacterium: Legionella pneumophila, genus novum, species nova, of the family Legionellaceae, familia nova. Ann Intern Med. 1979 Apr;90(4):656–658. doi: 10.7326/0003-4819-90-4-656. [DOI] [PubMed] [Google Scholar]
- Cianciotto N. P., Bangsborg J. M., Eisenstein B. I., Engleberg N. C. Identification of mip-like genes in the genus Legionella. Infect Immun. 1990 Sep;58(9):2912–2918. doi: 10.1128/iai.58.9.2912-2918.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Dondero T. J., Jr, Rendtorff R. C., Mallison G. F., Weeks R. M., Levy J. S., Wong E. W., Schaffner W. An outbreak of Legionnaires' disease associated with a contaminated air-conditioning cooling tower. N Engl J Med. 1980 Feb 14;302(7):365–370. doi: 10.1056/NEJM198002143020703. [DOI] [PubMed] [Google Scholar]
- Engleberg N. C., Carter C., Weber D. R., Cianciotto N. P., Eisenstein B. I. DNA sequence of mip, a Legionella pneumophila gene associated with macrophage infectivity. Infect Immun. 1989 Apr;57(4):1263–1270. doi: 10.1128/iai.57.4.1263-1270.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Jaulhac B., Nowicki M., Bornstein N., Meunier O., Prevost G., Piemont Y., Fleurette J., Monteil H. Detection of Legionella spp. in bronchoalveolar lavage fluids by DNA amplification. J Clin Microbiol. 1992 Apr;30(4):920–924. doi: 10.1128/jcm.30.4.920-924.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
- MacDonell M. T., Colwell R. R. The nucleotide sequence of the 5S rRNA from Legionella pneumophila. Nucleic Acids Res. 1987 Feb 11;15(3):1335–1335. doi: 10.1093/nar/15.3.1335. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Mahbubani M. H., Bej A. K., Miller R., Haff L., DiCesare J., Atlas R. M. Detection of Legionella with polymerase chain reaction and gene probe methods. Mol Cell Probes. 1990 Jun;4(3):175–187. doi: 10.1016/0890-8508(90)90051-z. [DOI] [PubMed] [Google Scholar]
- Selander R. K., McKinney R. M., Whittam T. S., Bibb W. F., Brenner D. J., Nolte F. S., Pattison P. E. Genetic structure of populations of Legionella pneumophila. J Bacteriol. 1985 Sep;163(3):1021–1037. doi: 10.1128/jb.163.3.1021-1037.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Starnbach M. N., Falkow S., Tompkins L. S. Species-specific detection of Legionella pneumophila in water by DNA amplification and hybridization. J Clin Microbiol. 1989 Jun;27(6):1257–1261. doi: 10.1128/jcm.27.6.1257-1261.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Stout J., Yu V. L., Vickers R. M., Zuravleff J., Best M., Brown A., Yee R. B., Wadowsky R. Ubiquitousness of Legionella pneumophila in the water supply of a hospital with endemic Legionnaires' disease. N Engl J Med. 1982 Feb 25;306(8):466–468. doi: 10.1056/NEJM198202253060807. [DOI] [PubMed] [Google Scholar]