Abstract
An increase in shell vial centrifugation force to 3,500 x g and a concomitant reduction in spin time to 15 min did not decrease the sensitivity of detecting viruses in clinical specimens compared with the accepted practice of using 700 x g for 40 min. No damage to the cell monolayer (ML) at the higher g force was observed. Toxicity to the ML is decreased with the shorter spin, probably because of reduced time of contact between the specimen and the ML.
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Selected References
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- Espy M. J., Smith T. F., Harmon M. W., Kendal A. P. Rapid detection of influenza virus by shell vial assay with monoclonal antibodies. J Clin Microbiol. 1986 Oct;24(4):677–679. doi: 10.1128/jcm.24.4.677-679.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Fedorko D. P., Ilstrup D. M., Smith T. F. Effect of age of shell vial monolayers on detection of cytomegalovirus from urine specimens. J Clin Microbiol. 1989 Sep;27(9):2107–2109. doi: 10.1128/jcm.27.9.2107-2109.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Gleaves C. A., Lee C. F., Bustamante C. I., Meyers J. D. Use of murine monoclonal antibodies for laboratory diagnosis of varicella-zoster virus infection. J Clin Microbiol. 1988 Sep;26(9):1623–1625. doi: 10.1128/jcm.26.9.1623-1625.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Gleaves C. A., Smith T. F., Shuster E. A., Pearson G. R. Rapid detection of cytomegalovirus in MRC-5 cells inoculated with urine specimens by using low-speed centrifugation and monoclonal antibody to an early antigen. J Clin Microbiol. 1984 Jun;19(6):917–919. doi: 10.1128/jcm.19.6.917-919.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Gleaves C. A., Wilson D. J., Wold A. D., Smith T. F. Detection and serotyping of herpes simplex virus in MRC-5 cells by use of centrifugation and monoclonal antibodies 16 h postinoculation. J Clin Microbiol. 1985 Jan;21(1):29–32. doi: 10.1128/jcm.21.1.29-32.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Mahafzah A. M., Landry M. L. Evaluation of immunofluorescent reagents, centrifugation, and conventional cultures for the diagnosis of adenovirus infection. Diagn Microbiol Infect Dis. 1989 Sep-Oct;12(5):407–411. doi: 10.1016/0732-8893(89)90111-9. [DOI] [PubMed] [Google Scholar]
- Matthey S., Nicholson D., Ruhs S., Alden B., Knock M., Schultz K., Schmuecker A. Rapid detection of respiratory viruses by shell vial culture and direct staining by using pooled and individual monoclonal antibodies. J Clin Microbiol. 1992 Mar;30(3):540–544. doi: 10.1128/jcm.30.3.540-544.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Paya C. V., Wold A. D., Smith T. F. Detection of cytomegalovirus infections in specimens other than urine by the shell vial assay and conventional tube cell cultures. J Clin Microbiol. 1987 May;25(5):755–757. doi: 10.1128/jcm.25.5.755-757.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Ripa K. T., Mårdh P. A. Cultivation of Chlamydia trachomatis in cycloheximide-treated mccoy cells. J Clin Microbiol. 1977 Oct;6(4):328–331. doi: 10.1128/jcm.6.4.328-331.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Salmon V. C., Turner R. B., Speranza M. J., Overall J. C., Jr Rapid detection of herpes simplex virus in clinical specimens by centrifugation and immunoperoxidase staining. J Clin Microbiol. 1986 Apr;23(4):683–686. doi: 10.1128/jcm.23.4.683-686.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Shuster E. A., Beneke J. S., Tegtmeier G. E., Pearson G. R., Gleaves C. A., Wold A. D., Smith T. F. Monoclonal antibody for rapid laboratory detection of cytomegalovirus infections: characterization and diagnostic application. Mayo Clin Proc. 1985 Sep;60(9):577–585. doi: 10.1016/s0025-6196(12)60979-3. [DOI] [PubMed] [Google Scholar]
- Smith M. C., Creutz C., Huang Y. T. Detection of respiratory syncytial virus in nasopharyngeal secretions by shell vial technique. J Clin Microbiol. 1991 Mar;29(3):463–465. doi: 10.1128/jcm.29.3.463-465.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]