Abstract
A collection of 150 Neisseria gonorrhoeae isolates from Africa, where various resistance mechanisms among N. gonorrhoeae isolates are common, was used to the compare E test (AB Biodisk, Solna, Sweden) with agar dilution susceptibility testing. MICs obtained by the E test agreed within 1 log2 concentration by the agar dilution method for 97.5, 97.3, 96.6, 94, and 84.7% of the tested isolates for penicillin, ciprofloxacin, chloramphenicol, tetracycline, and trimethoprim-sulfamethoxazole, respectively. No significant difference in susceptibility categorization was observed between either method. The E test is an attractive alternative to the agar dilution technique and is a more appropriate method for N. gonorrhoeae susceptibility testing in developing countries.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Doern G. V., Jones R. N. Antimicrobial susceptibility testing of Haemophilus influenzae, Branhamella catarrhalis, and Neisseria gonorrhoeae. Antimicrob Agents Chemother. 1988 Dec;32(12):1747–1753. doi: 10.1128/aac.32.12.1747. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Easmon C. S. Gonococcal resistance to antibiotics. J Antimicrob Chemother. 1985 Oct;16(4):409–412. doi: 10.1093/jac/16.4.409. [DOI] [PubMed] [Google Scholar]
- Gorwitz R. J., Nakashima A. K., Moran J. S., Knapp J. S. Sentinel surveillance for antimicrobial resistance in Neisseria gonorrhoeae--United States, 1988-1991. The Gonococcal Isolate Surveillance Project Study Group. MMWR CDC Surveill Summ. 1993 Aug 13;42(3):29–39. [PubMed] [Google Scholar]
- Johnson S. R., Morse S. A. Antibiotic resistance in Neisseria gonorrhoeae: genetics and mechanisms of resistance. Sex Transm Dis. 1988 Oct-Dec;15(4):217–224. doi: 10.1097/00007435-198810000-00008. [DOI] [PubMed] [Google Scholar]
- Jorgensen J. H., Howell A. W., Maher L. A. Quantitative antimicrobial susceptibility testing of Haemophilus influenzae and Streptococcus pneumoniae by using the E-test. J Clin Microbiol. 1991 Jan;29(1):109–114. doi: 10.1128/jcm.29.1.109-114.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
- KELLOGG D. S., Jr, PEACOCK W. L., Jr, DEACON W. E., BROWN L., PIRKLE D. I. NEISSERIA GONORRHOEAE. I. VIRULENCE GENETICALLY LINKED TO CLONAL VARIATION. J Bacteriol. 1963 Jun;85:1274–1279. doi: 10.1128/jb.85.6.1274-1279.1963. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Knapp J. S., Zenilman J. M., Biddle J. W., Perkins G. H., DeWitt W. E., Thomas M. L., Johnson S. R., Morse S. A. Frequency and distribution in the United States of strains of Neisseria gonorrhoeae with plasmid-mediated, high-level resistance to tetracycline. J Infect Dis. 1987 Apr;155(4):819–822. doi: 10.1093/infdis/155.4.819. [DOI] [PubMed] [Google Scholar]
- Sanchez M. L., Barrett M. S., Jones R. N. The E-Test applied to susceptibility tests of gonococci, multiply-resistant enterococci, and Enterobacteriaceae producing potent beta-lactamases. Diagn Microbiol Infect Dis. 1992 Jul;15(5):459–463. doi: 10.1016/0732-8893(92)90090-g. [DOI] [PubMed] [Google Scholar]
- Yeung K. H., Ng L. K., Dillon J. A. Evaluation of Etest for testing antimicrobial susceptibilities of Neisseria gonorrhoeae isolates with different growth media. J Clin Microbiol. 1993 Nov;31(11):3053–3055. doi: 10.1128/jcm.31.11.3053-3055.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]