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Journal of Clinical Microbiology logoLink to Journal of Clinical Microbiology
. 1994 Jun;32(6):1589–1590. doi: 10.1128/jcm.32.6.1589-1590.1994

Development and evaluation of rapid monoclonal antibody-based coagglutination test for direct detection of Vibrio cholerae O139 synonym Bengal in stool samples.

F Qadri 1, A Chowdhury 1, J Hossain 1, K Chowdhury 1, T Azim 1, T Shimada 1, K M Islam 1, R B Sack 1, M J Albert 1
PMCID: PMC264045  PMID: 8077410

Abstract

A monoclonal antibody-based coagglutination test directly detected Vibrio cholerae O139 synonym Bengal in 83 of 120 watery diarrheal stool specimens; on culture, 90 samples were positive. Thus, with 92% sensitivity, 100% specificity, and 100% positive and 95% negative predictive values, the coagglutination test is a useful rapid test for V. cholerae O139.

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Selected References

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  1. Albert M. J., Siddique A. K., Islam M. S., Faruque A. S., Ansaruzzaman M., Faruque S. M., Sack R. B. Large outbreak of clinical cholera due to Vibrio cholerae non-O1 in Bangladesh. Lancet. 1993 Mar 13;341(8846):704–704. doi: 10.1016/0140-6736(93)90481-u. [DOI] [PubMed] [Google Scholar]
  2. Bhattacharya M. K., Bhattacharya S. K., Garg S., Saha P. K., Dutta D., Nair G. B., Deb B. C., Das K. P. Outbreak of Vibrio cholerae non-O1 in India and Bangladesh. Lancet. 1993 May 22;341(8856):1346–1347. doi: 10.1016/0140-6736(93)90855-b. [DOI] [PubMed] [Google Scholar]
  3. Colwell R. R., Hasan J. A., Huq A., Loomis L., Siebeling R. J., Torres M., Galvez S., Islam S., Tamplin M. T., Bernstein D. Development and evaluation of a rapid, simple, sensitive, monoclonal antibody-based co-agglutination test for direct detection of Vibrio cholerae 01. FEMS Microbiol Lett. 1992 Oct 15;76(3):215–219. doi: 10.1016/0378-1097(92)90339-p. [DOI] [PubMed] [Google Scholar]
  4. Janda J. M., Powers C., Bryant R. G., Abbott S. L. Current perspectives on the epidemiology and pathogenesis of clinically significant Vibrio spp. Clin Microbiol Rev. 1988 Jul;1(3):245–267. doi: 10.1128/cmr.1.3.245. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Qadri F., Azim T., Chowdhury A., Hossain J., Sack R. B., Albert M. J. Production, characterization, and application of monoclonal antibodies to Vibrio cholerae O139 synonym Bengal. Clin Diagn Lab Immunol. 1994 Jan;1(1):51–54. doi: 10.1128/cdli.1.1.51-54.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Rahman M., Sack D. A., Mahmood S., Hossain A. Rapid diagnosis of cholera by coagglutination test using 4-h fecal enrichment cultures. J Clin Microbiol. 1987 Nov;25(11):2204–2206. doi: 10.1128/jcm.25.11.2204-2206.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Ramamurthy T., Garg S., Sharma R., Bhattacharya S. K., Nair G. B., Shimada T., Takeda T., Karasawa T., Kurazano H., Pal A. Emergence of novel strain of Vibrio cholerae with epidemic potential in southern and eastern India. Lancet. 1993 Mar 13;341(8846):703–704. doi: 10.1016/0140-6736(93)90480-5. [DOI] [PubMed] [Google Scholar]
  8. Swerdlow D. L., Ries A. A. Vibrio cholerae non-O1--the eighth pandemic? Lancet. 1993 Aug 14;342(8868):382–383. doi: 10.1016/0140-6736(93)92806-5. [DOI] [PubMed] [Google Scholar]

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