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. 1994 Jun;32(6):1591–1593. doi: 10.1128/jcm.32.6.1591-1593.1994

Use of arbitrary primer PCR to type Clostridium difficile and comparison of results with those by immunoblot typing.

G E Killgore 1, H Kato 1
PMCID: PMC264046  PMID: 7741841

Abstract

An arbitrarily primed PCR (AP-PCR) assay was used to type Clostridium difficile isolates from a hospital outbreak of antibiotic-associated diarrhea. Forty-one isolates were separated into nine groups, with 66% falling into one group; no other group contained more than 10%. Comparison of AP-PCR grouping with that when the immunoblot technique was used showed agreement for 33 of 34 isolates typed by both techniques, and AP-PCR grouped seven isolates that were not typeable by immunoblotting.

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Selected References

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