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. 1994 Jun;32(6):1594–1596. doi: 10.1128/jcm.32.6.1594-1596.1994

Comparison of agar dilution, broth dilution, and disk diffusion testing of ampicillin against Haemophilus species by using in-house and commercially prepared media.

P C Kohner 1, J E Rosenblatt 1, F R Cockerill 3rd 1
PMCID: PMC264047  PMID: 8077411

Abstract

An evaluation to determine the optimal method for the in vitro susceptibility testing of Haemophilus strains to ampicillin was undertaken. In our hands, in-house-prepared Haemophilus Test Medium used by either the broth macrodilution or agar dilution method produced the most consistent results, especially for beta-lactamase-negative, ampicillin-resistant H. influenzae strains.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Barry A. L., Jorgensen J. H., Hardy D. J., Allen S. D., Baker C. N. Haemophilus influenzae ATCC 49766, an alternative quality control strain for monitoring broth microdilution susceptibility tests with selected beta-lactams. J Clin Microbiol. 1992 Aug;30(8):2033–2037. doi: 10.1128/jcm.30.8.2033-2037.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Doern G. V., Gerlach E. H., Jorgensen J. H., Murray P. R., Thornsberry C., Washington J. A., Jr Quality control limits for disk diffusion and broth microdilution susceptibility tests with Haemophilus test medium. Diagn Microbiol Infect Dis. 1991 Nov-Dec;14(6):485–493. doi: 10.1016/0732-8893(91)90004-y. [DOI] [PubMed] [Google Scholar]
  3. Doern G. V. In vitro susceptibility testing of Haemophilus influenzae: review of new National Committee for Clinical Laboratory Standards recommendations. J Clin Microbiol. 1992 Dec;30(12):3035–3038. doi: 10.1128/jcm.30.12.3035-3038.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Doern G. V., Jorgensen J. H., Thornsberry C., Preston D. A., Tubert T., Redding J. S., Maher L. A. National collaborative study of the prevalence of antimicrobial resistance among clinical isolates of Haemophilus influenzae. Antimicrob Agents Chemother. 1988 Feb;32(2):180–185. doi: 10.1128/aac.32.2.180. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Doern G. V., Jorgensen J. H., Thornsberry C., Snapper H. Disk diffusion susceptibility testing of Haemophilus influenzae using haemophilus test medium. Eur J Clin Microbiol Infect Dis. 1990 May;9(5):329–336. doi: 10.1007/BF01973739. [DOI] [PubMed] [Google Scholar]
  6. Heelan J. S., Chesney D., Guadagno G. Investigation of ampicillin-intermediate strains of Haemophilus influenzae by using the disk diffusion procedure and current National Committee for Clinical Laboratory Standards guidelines. J Clin Microbiol. 1992 Jul;30(7):1674–1677. doi: 10.1128/jcm.30.7.1674-1677.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Jorgensen J. H., Barry A. L., Doern G. V., Ferraro M. J., Murray P. R. Development of revised quality control limits for disk diffusion susceptibility tests of selected cephem antibiotics with Haemophilus influenzae and description of a new control strain. J Clin Microbiol. 1992 Aug;30(8):2029–2032. doi: 10.1128/jcm.30.8.2029-2032.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Jorgensen J. H., Doern G. V., Thornsberry C., Preston D. A., Redding J. S., Maher L. A., Tubert T. Susceptibility of multiply resistant Haemophilus influenzae to newer antimicrobial agents. Diagn Microbiol Infect Dis. 1988 Jan;9(1):27–32. doi: 10.1016/0732-8893(88)90057-0. [DOI] [PubMed] [Google Scholar]
  9. Jorgensen J. H., Howell A. W., Maher L. A. Antimicrobial susceptibility testing of less commonly isolated Haemophilus species using Haemophilus test medium. J Clin Microbiol. 1990 May;28(5):985–988. doi: 10.1128/jcm.28.5.985-988.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Mendelman P. M., Henritzy L. L., Chaffin D. O., Lent K., Smith A. L., Stull T. L., Wiley E. A. In vitro activities and targets of three cephem antibiotics against Haemophilus influenzae. Antimicrob Agents Chemother. 1989 Nov;33(11):1878–1882. doi: 10.1128/aac.33.11.1878. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Mendelman P. M., Wiley E. A., Stull T. L., Clausen C., Chaffin D. O., Onay O. Problems with current recommendations for susceptibility testing of Haemophilus influenzae. Antimicrob Agents Chemother. 1990 Aug;34(8):1480–1484. doi: 10.1128/aac.34.8.1480. [DOI] [PMC free article] [PubMed] [Google Scholar]

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