Skip to main content
PMC home page
Journal of Clinical Microbiology logoLink to Journal of Clinical Microbiology
. 1994 Oct;32(10):2350–2352. doi: 10.1128/jcm.32.10.2350-2352.1994

Diagnosis of genital Chlamydia trachomatis infections in asymptomatic males by testing urine by PCR.

M Domeika 1, M Bassiri 1, P A Mårdh 1
PMCID: PMC264064  PMID: 7814464

Abstract

An enzyme-linked immunosorbent assay (EIA) (MikroTrak; Syva) was compared with PCR (Amplicor; Roche) for detection of Chlamydia trachomatis in first-void urine (FVU) from 184 men attending a skin and venereal disease clinic. The prevalence of C. trachomatis in the population studied was 18.5%. Discrepant results between Syva EIA and Roche PCR were retested by using major outer membrane protein primer-based PCR. After retesting, the sensitivity, the specificity, and the positive and negative predictive values for the Syva EIA were 85.3, 100, 100, and 77.5%, respectively, and those for the Roche PCR 100, 100, 100, and 100%, respectively. It was concluded that PCR provides a highly sensitive and specific noninvasive screening method for genital chlamydial infection in asymptomatic men.

Full text

PDF
2350

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bauwens J. E., Clark A. M., Loeffelholz M. J., Herman S. A., Stamm W. E. Diagnosis of Chlamydia trachomatis urethritis in men by polymerase chain reaction assay of first-catch urine. J Clin Microbiol. 1993 Nov;31(11):3013–3016. doi: 10.1128/jcm.31.11.3013-3016.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bobo L., Coutlee F., Yolken R. H., Quinn T., Viscidi R. P. Diagnosis of Chlamydia trachomatis cervical infection by detection of amplified DNA with an enzyme immunoassay. J Clin Microbiol. 1990 Sep;28(9):1968–1973. doi: 10.1128/jcm.28.9.1968-1973.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Bobo L., Munoz B., Viscidi R., Quinn T., Mkocha H., West S. Diagnosis of Chlamydia trachomatis eye infection in Tanzania by polymerase chain reaction/enzyme immunoassay. Lancet. 1991 Oct 5;338(8771):847–850. doi: 10.1016/0140-6736(91)91502-l. [DOI] [PubMed] [Google Scholar]
  4. Caul E. O., Paul I. D., Milne J. D., Crowley T. Non-invasive sampling method for detecting Chlamydia trachomatis. Lancet. 1988 Nov 26;2(8622):1246–1247. doi: 10.1016/s0140-6736(88)90831-8. [DOI] [PubMed] [Google Scholar]
  5. Chernesky M., Castriciano S., Sellors J., Stewart I., Cunningham I., Landis S., Seidelman W., Grant L., Devlin C., Mahony J. Detection of Chlamydia trachomatis antigens in urine as an alternative to swabs and cultures. J Infect Dis. 1990 Jan;161(1):124–126. doi: 10.1093/infdis/161.1.124. [DOI] [PubMed] [Google Scholar]
  6. Cimino G. D., Metchette K. C., Tessman J. W., Hearst J. E., Isaacs S. T. Post-PCR sterilization: a method to control carryover contamination for the polymerase chain reaction. Nucleic Acids Res. 1991 Jan 11;19(1):99–107. doi: 10.1093/nar/19.1.99. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Claas H. C., Wagenvoort J. H., Niesters H. G., Tio T. T., Van Rijsoort-Vos J. H., Quint W. G. Diagnostic value of the polymerase chain reaction for Chlamydia detection as determined in a follow-up study. J Clin Microbiol. 1991 Jan;29(1):42–45. doi: 10.1128/jcm.29.1.42-45.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Domeika M. A., Bassiri M., Mårdh P. A. Non-invasive sampling for detection of genital infection with Chlamydia trachomatis in males utilising urinary leukocyte esterase tests and immunoassays. Infection. 1994 Mar-Apr;22(2):65–68. doi: 10.1007/BF01739005. [DOI] [PubMed] [Google Scholar]
  9. Genç M., Domeika M., Mårdh P. A. Cost-effectiveness of screening for Chlamydia using DNA amplification. JAMA. 1994 Jun 8;271(22):1741–1741. doi: 10.1001/jama.1994.03510460033017. [DOI] [PubMed] [Google Scholar]
  10. Genç M., Ruusuvaara L., Mårdh P. A. An economic evaluation of screening for Chlamydia trachomatis in adolescent males. JAMA. 1993 Nov 3;270(17):2057–2064. doi: 10.1001/jama.1993.03510170047029. [DOI] [PubMed] [Google Scholar]
  11. Jaschek G., Gaydos C. A., Welsh L. E., Quinn T. C. Direct detection of Chlamydia trachomatis in urine specimens from symptomatic and asymptomatic men by using a rapid polymerase chain reaction assay. J Clin Microbiol. 1993 May;31(5):1209–1212. doi: 10.1128/jcm.31.5.1209-1212.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Jawad A. J., Manuel G., Matthews R., Wise R., Clay J. C. Evaluation of a genus-specific monoclonal antibody in an amplified enzyme-linked immunoassay in the detection of Chlamydia in urine samples from men. Sex Transm Dis. 1990 Apr-Jun;17(2):87–89. doi: 10.1097/00007435-199004000-00008. [DOI] [PubMed] [Google Scholar]
  13. Leonardi G. P., Seitz M., Edstrom R., Cruz J., Costello P., Szabo K. Evaluation of three immunoassays for detection of Chlamydia trachomatis in urine specimens from asymptomatic males. J Clin Microbiol. 1992 Nov;30(11):2793–2796. doi: 10.1128/jcm.30.11.2793-2796.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Loeffelholz M. J., Lewinski C. A., Silver S. R., Purohit A. P., Herman S. A., Buonagurio D. A., Dragon E. A. Detection of Chlamydia trachomatis in endocervical specimens by polymerase chain reaction. J Clin Microbiol. 1992 Nov;30(11):2847–2851. doi: 10.1128/jcm.30.11.2847-2851.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Mahony J. B., Luinstra K. E., Jang D., Sellors J., Chernesky M. A. Chlamydia trachomatis confirmatory testing of PCR-positive genitourinary specimens using a second set of plasmid primers. Mol Cell Probes. 1992 Oct;6(5):381–388. doi: 10.1016/0890-8508(92)90031-r. [DOI] [PubMed] [Google Scholar]
  16. Mahony J. B., Luinstra K. E., Waner J., McNab G., Hobranzska H., Gregson D., Sellors J. W., Chernesky M. A. Interlaboratory agreement study of a double set of PCR plasmid primers for detection of Chlamydia trachomatis in a variety of genitourinary specimens. J Clin Microbiol. 1994 Jan;32(1):87–91. doi: 10.1128/jcm.32.1.87-91.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Ostergaard L., Birkelund S., Christiansen G. Use of polymerase chain reaction for detection of Chlamydia trachomatis. J Clin Microbiol. 1990 Jun;28(6):1254–1260. doi: 10.1128/jcm.28.6.1254-1260.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Paul I. D., Caul E. O. Evaluation of three Chlamydia trachomatis immunoassays with an unbiased, noninvasive clinical sample. J Clin Microbiol. 1990 Feb;28(2):220–222. doi: 10.1128/jcm.28.2.220-222.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Ridgway G. L., Taylor-Robinson D. Current problems in microbiology: 1. Chlamydial infections: which laboratory test? J Clin Pathol. 1991 Jan;44(1):1–5. doi: 10.1136/jcp.44.1.1. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Schachter J., Stamm W. E., Chernesky M. A., Hook E. W., 3rd, Jones R. B., Judson F. N., Kellogg J. A., LeBar B., Mårdh P. A., McCormack W. M. Nonculture tests for genital tract chlamydial infection. What does the package insert mean, and will it mean the same thing tomorrow? Sex Transm Dis. 1992 Sep-Oct;19(5):243–244. [PubMed] [Google Scholar]
  21. Stamm W. E. Diagnosis of Chlamydia trachomatis genitourinary infections. Ann Intern Med. 1988 May;108(5):710–717. doi: 10.7326/0003-4819-108-5-710. [DOI] [PubMed] [Google Scholar]
  22. Williams T. W., Tyler S. D., Giercke S., Pollard D. R., McNicol P., Rozee K. R. Comparison of polymerase chain reaction and chlamydiazyme for the detection of Chlamydia trachomatis in clinical specimens. Eur J Clin Microbiol Infect Dis. 1992 Mar;11(3):233–236. doi: 10.1007/BF02098085. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Clinical Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES