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. 1994 Oct;32(10):2404–2406. doi: 10.1128/jcm.32.10.2404-2406.1994

Rapid diagnosis of schistosomiasis by antigen detection in urine with a reagent strip.

L van Etten 1, C C Folman 1, T A Eggelte 1, P G Kremsner 1, A M Deelder 1
PMCID: PMC264074  PMID: 7814474

Abstract

For the rapid and simple diagnosis of schistosomiasis, a reagent strip assay for detection of circulating cathodic antigen (CCA) in urine was developed. The test was based on a previously developed sandwich enzyme-linked immunosorbent assay (ELISA) using a combination of two anti-CCA monoclonal antibodies. For the reagent strip assay, as the capture matrix, monoclonal antibody was coated onto a nitrocellulose membrane and mounted on polyvinyl chloride strips. Urine samples were then tested in an assay consisting of a combined incubation step of the urine sample and biotinylated detecting antibody followed by incubation in streptavidin peroxidase and a subsequent staining. The specificity and the sensitivity of the assay, as determined with urine samples of 61 uninfected controls and 67 Schistosoma mansoni-infected individuals, were 96.7 and 95.5%, respectively. The results of the reagent strip assay compared very well with microscopical parasitological diagnosis by the standard Kato-Katz method for the same individuals. The reagent strip test has a lower detection limit of 1 ng of CCA per ml and can be completed in 75 min. By the inclusion of two reference bands on the strips, standardized reading could be achieved. This reagent strip assay is a promising tool for qualitative diagnosis of S. mansoni infections in control programs.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Boctor F. N., Stek M. J., Jr, Peter J. B., Kamal R. Simplification and standardization of dot-ELISA for human schistosomiasis mansoni. J Parasitol. 1987 Jun;73(3):589–592. [PubMed] [Google Scholar]
  2. De Jonge N., Fillié Y. E., Hilberath G. W., Krijger F. W., Lengeler C., de Savigny D. H., van Vliet N. G., Deelder A. M. Presence of the schistosome circulating anodic antigen (CAA) in urine of patients with Schistosoma mansoni or S. haematobium infections. Am J Trop Med Hyg. 1989 Nov;41(5):563–569. doi: 10.4269/ajtmh.1989.41.563. [DOI] [PubMed] [Google Scholar]
  3. Deelder A. M., De Jonge N., Boerman O. C., Fillié Y. E., Hilberath G. W., Rotmans J. P., Gerritse M. J., Schut D. W. Sensitive determination of circulating anodic antigen in Schistosoma mansoni infected individuals by an enzyme-linked immunosorbent assay using monoclonal antibodies. Am J Trop Med Hyg. 1989 Mar;40(3):268–272. doi: 10.4269/ajtmh.1989.40.268. [DOI] [PubMed] [Google Scholar]
  4. Katz N., Chaves A., Pellegrino J. A simple device for quantitative stool thick-smear technique in Schistosomiasis mansoni. Rev Inst Med Trop Sao Paulo. 1972 Nov-Dec;14(6):397–400. [PubMed] [Google Scholar]
  5. Kremsner P. G., Enyong P., Krijger F. W., De Jonge N., Zotter G. M., Thalhammer F., Mühlschlegel F., Bienzle U., Feldmeier H., Deelder A. M. Circulating anodic and cathodic antigen in serum and urine from Schistosoma haematobium-infected Cameroonian children receiving praziquantel: a longitudinal study. Clin Infect Dis. 1994 Mar;18(3):408–413. doi: 10.1093/clinids/18.3.408. [DOI] [PubMed] [Google Scholar]
  6. Krijger F. W., van Lieshout L., Deelder A. M. A simple technique to pretreat urine and serum samples for quantitation of schistosome circulating anodic and cathodic antigen. Acta Trop. 1994 Feb;56(1):55–63. doi: 10.1016/0001-706x(94)90040-x. [DOI] [PubMed] [Google Scholar]
  7. Pappas M. G., Hajkowski R., Hockmeyer W. T. Dot enzyme-linked immunosorbent assay (Dot-ELISA): a micro technique for the rapid diagnosis of visceral leishmaniasis. J Immunol Methods. 1983 Nov 11;64(1-2):205–214. doi: 10.1016/0022-1759(83)90399-x. [DOI] [PubMed] [Google Scholar]
  8. Pappas M. G. Recent applications of the Dot-ELISA in immunoparasitology. Vet Parasitol. 1988 Sep;29(2-3):105–129. doi: 10.1016/0304-4017(88)90120-3. [DOI] [PubMed] [Google Scholar]
  9. Polderman A. M., Mpamila K., Manshande J. P., Gryseels B., van Schayk O. Historical, geological and ecological aspects of transmission of intestinal schistosomiasis in Maniema, Kivu Province, Zaire. Ann Soc Belg Med Trop. 1985 Sep;65(3):251–261. [PubMed] [Google Scholar]
  10. Rabello A. L., Dias Neto E., Garcia M. M., Katz N. DOT-dye-immunoassay for the diagnosis of schistosomiasis mansoni. Mem Inst Oswaldo Cruz. 1992 Apr-Jun;87(2):187–190. doi: 10.1590/s0074-02761992000200003. [DOI] [PubMed] [Google Scholar]
  11. Savioli L., Hatz C., Dixon H., Kisumku U. M., Mott K. E. Control of morbidity due to Schistosoma haematobium on Pemba Island: egg excretion and hematuria as indicators of infection. Am J Trop Med Hyg. 1990 Sep;43(3):289–295. doi: 10.4269/ajtmh.1990.43.289. [DOI] [PubMed] [Google Scholar]
  12. Snowden K., Hommel M. Antigen detection immunoassay using dipsticks and colloidal dyes. J Immunol Methods. 1991 Jun 24;140(1):57–65. doi: 10.1016/0022-1759(91)90126-z. [DOI] [PubMed] [Google Scholar]
  13. Stott D. I. Immunoblotting and dot blotting. J Immunol Methods. 1989 May 12;119(2):153–187. doi: 10.1016/0022-1759(89)90394-3. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Van 't Wout A. B., De Jonge N., Tiu W. U., Garcia E. E., Mitchell G. F., Deelder A. M. Schistosome circulating anodic antigen in serum of individuals infected with Schistosoma japonicum from the Philippines before and after chemotherapy with praziquantel. Trans R Soc Trop Med Hyg. 1992 Jul-Aug;86(4):410–413. doi: 10.1016/0035-9203(92)90243-6. [DOI] [PubMed] [Google Scholar]
  15. de Jonge N., Kremsner P. G., Krijger F. W., Schommer G., Fillié Y. E., Kornelis D., van Zeyl R. J., van Dam G. J., Feldmeier H., Deelder A. M. Detection of the schistosome circulating cathodic antigen by enzyme immunoassay using biotinylated monoclonal antibodies. Trans R Soc Trop Med Hyg. 1990 Nov-Dec;84(6):815–818. doi: 10.1016/0035-9203(90)90094-u. [DOI] [PubMed] [Google Scholar]
  16. van Dam G. J., Seino J., Rotmans J. P., Daha M. R., Deelder A. M. Schistosoma mansoni circulating anodic antigen but not circulating cathodic antigen interacts with complement component C1q. Eur J Immunol. 1993 Nov;23(11):2807–2812. doi: 10.1002/eji.1830231113. [DOI] [PubMed] [Google Scholar]
  17. van Lieshout L., De Jonge N., Mansour M. M., Bassily S., Krijger F. W., Deelder A. M. Circulating cathodic antigen levels in serum and urine of schistosomiasis patients before and after chemotherapy with praziquantel. Trans R Soc Trop Med Hyg. 1993 May-Jun;87(3):311–312. doi: 10.1016/0035-9203(93)90142-d. [DOI] [PubMed] [Google Scholar]

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