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Journal of Clinical Microbiology logoLink to Journal of Clinical Microbiology
. 1994 Oct;32(10):2590–2592. doi: 10.1128/jcm.32.10.2590-2592.1994

Diagnosis of enteroviral meningitis by using PCR with a colorimetric microwell detection assay.

H A Rotbart 1, M H Sawyer 1, S Fast 1, C Lewinski 1, N Murphy 1, E F Keyser 1, J Spadoro 1, S Y Kao 1, M Loeffelholz 1
PMCID: PMC264111  PMID: 7814507

Abstract

A 5-h, user-friendly PCR assay for the diagnosis of enteroviral meningitis was developed. Reverse transcription and amplification were performed in a one-step reaction using rTth polymerase. Carryover contamination was prevented with dUTP and uracil N-glycosylate. Detection was performed colorimetrically on a microwell titer plate. Sensitivity, specificity, positive predictive value, and negative predictive value were 94.7, 97.4, 94.7, and 97.4%, respectively.

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Selected References

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  1. Berlin L. E., Rorabaugh M. L., Heldrich F., Roberts K., Doran T., Modlin J. F. Aseptic meningitis in infants < 2 years of age: diagnosis and etiology. J Infect Dis. 1993 Oct;168(4):888–892. doi: 10.1093/infdis/168.4.888. [DOI] [PubMed] [Google Scholar]
  2. Chonmaitree T., Menegus M. A., Powell K. R. The clinical relevance of 'CSF viral culture'. A two-year experience with aseptic meningitis in Rochester, NY. JAMA. 1982 Apr 2;247(13):1843–1847. [PubMed] [Google Scholar]
  3. Loeffelholz M. J., Lewinski C. A., Silver S. R., Purohit A. P., Herman S. A., Buonagurio D. A., Dragon E. A. Detection of Chlamydia trachomatis in endocervical specimens by polymerase chain reaction. J Clin Microbiol. 1992 Nov;30(11):2847–2851. doi: 10.1128/jcm.30.11.2847-2851.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Longo M. C., Berninger M. S., Hartley J. L. Use of uracil DNA glycosylase to control carry-over contamination in polymerase chain reactions. Gene. 1990 Sep 1;93(1):125–128. doi: 10.1016/0378-1119(90)90145-h. [DOI] [PubMed] [Google Scholar]
  5. Olive D. M., Al-Mufti S., Al-Mulla W., Khan M. A., Pasca A., Stanway G., Al-Nakib W. Detection and differentiation of picornaviruses in clinical samples following genomic amplification. J Gen Virol. 1990 Sep;71(Pt 9):2141–2147. doi: 10.1099/0022-1317-71-9-2141. [DOI] [PubMed] [Google Scholar]
  6. Rotbart H. A. Diagnosis of enteroviral meningitis with the polymerase chain reaction. J Pediatr. 1990 Jul;117(1 Pt 1):85–89. doi: 10.1016/s0022-3476(05)82451-5. [DOI] [PubMed] [Google Scholar]
  7. Rotbart H. A. Enzymatic RNA amplification of the enteroviruses. J Clin Microbiol. 1990 Mar;28(3):438–442. doi: 10.1128/jcm.28.3.438-442.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Sawyer M. H., Holland D., Aintablian N., Connor J. D., Keyser E. F., Waecker N. J., Jr Diagnosis of enteroviral central nervous system infection by polymerase chain reaction during a large community outbreak. Pediatr Infect Dis J. 1994 Mar;13(3):177–182. doi: 10.1097/00006454-199403000-00002. [DOI] [PubMed] [Google Scholar]
  9. Strikas R. A., Anderson L. J., Parker R. A. Temporal and geographic patterns of isolates of nonpolio enterovirus in the United States, 1970-1983. J Infect Dis. 1986 Feb;153(2):346–351. doi: 10.1093/infdis/153.2.346. [DOI] [PubMed] [Google Scholar]
  10. Young K. K., Resnick R. M., Myers T. W. Detection of hepatitis C virus RNA by a combined reverse transcription-polymerase chain reaction assay. J Clin Microbiol. 1993 Apr;31(4):882–886. doi: 10.1128/jcm.31.4.882-886.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Zoll G. J., Melchers W. J., Kopecka H., Jambroes G., van der Poel H. J., Galama J. M. General primer-mediated polymerase chain reaction for detection of enteroviruses: application for diagnostic routine and persistent infections. J Clin Microbiol. 1992 Jan;30(1):160–165. doi: 10.1128/jcm.30.1.160-165.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]

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