Skip to main content
NCBI home page
Journal of Clinical Microbiology logoLink to Journal of Clinical Microbiology
. 1994 Oct;32(10):2613–2615. doi: 10.1128/jcm.32.10.2613-2615.1994

Hepatitis C virus (HCV)-specific in vitro antibody secretion by peripheral blood lymphocytes: correlation with progression of disease and HCV RNA in HCV antibody-positive patients.

J Ducos 1, A M Bianchi-Mondain 1, M Francois 1, M Boisset 1, J P Vendrell 1, F Barin 1, A Serre 1, D Larrey 1
PMCID: PMC264118  PMID: 7529245

Abstract

Hepatitis C virus-specific in vitro antibody production (HCV IVAP) by peripheral blood lymphocytes in 53 HCV antibody-positive patients was investigated in comparison with alanine aminotransferase (ALT) levels and HCV RNA in serum samples. All 29 HCV IVAP-positive patients were HCV RNA positive; 26 had elevated ALT levels. Among the 24 HCV IVAP-negative patients, 16 were HCV RNA negative, with 12 presenting normal ALT values. These data indicate that HCV IVAP results are highly correlated (P < 0.001) with HCV RNA results and ALT levels. Our study indicates that HCV IVAP can be used as a novel assay in the diagnosis and pathogenesis exploration of HCV infection.

Full text

PDF
2613

Images in this article

2614Image
on p.2614

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Alter H. J., Purcell R. H., Shih J. W., Melpolder J. C., Houghton M., Choo Q. L., Kuo G. Detection of antibody to hepatitis C virus in prospectively followed transfusion recipients with acute and chronic non-A, non-B hepatitis. N Engl J Med. 1989 Nov 30;321(22):1494–1500. doi: 10.1056/NEJM198911303212202. [DOI] [PubMed] [Google Scholar]
  2. Cha T. A., Kolberg J., Irvine B., Stempien M., Beall E., Yano M., Choo Q. L., Houghton M., Kuo G., Han J. H. Use of a signature nucleotide sequence of hepatitis C virus for detection of viral RNA in human serum and plasma. J Clin Microbiol. 1991 Nov;29(11):2528–2534. doi: 10.1128/jcm.29.11.2528-2534.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Craske J., Paver W. K., Farmer D. An algorithm for confirming screen reactivity in blood donors in enzyme immunoassays for antibodies to hepatitis C virus. J Immunol Methods. 1993 Apr 2;160(2):227–235. doi: 10.1016/0022-1759(93)90182-7. [DOI] [PubMed] [Google Scholar]
  4. Farci P., Alter H. J., Wong D., Miller R. H., Shih J. W., Jett B., Purcell R. H. A long-term study of hepatitis C virus replication in non-A, non-B hepatitis. N Engl J Med. 1991 Jul 11;325(2):98–104. doi: 10.1056/NEJM199107113250205. [DOI] [PubMed] [Google Scholar]
  5. François M., Dubois F., Brand D., Bacq Y., Guerois C., Mouchet C., Tichet J., Goudeau A., Barin F. Prevalence and significance of hepatitis C virus (HCV) viremia in HCV antibody-positive subjects from various populations. J Clin Microbiol. 1993 May;31(5):1189–1193. doi: 10.1128/jcm.31.5.1189-1193.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Garson J. A., Ring C. J., Tuke P. W. Improvement of HCV genome detection with "short" PCR products. Lancet. 1991 Dec 7;338(8780):1466–1467. doi: 10.1016/0140-6736(91)92775-w. [DOI] [PubMed] [Google Scholar]
  7. Garson J. A., Ring C., Tuke P., Tedder R. S. Enhanced detection by PCR of hepatitis C virus RNA. Lancet. 1990 Oct 6;336(8719):878–879. doi: 10.1016/0140-6736(90)92384-t. [DOI] [PubMed] [Google Scholar]
  8. Garson J. A., Tedder R. S., Briggs M., Tuke P., Glazebrook J. A., Trute A., Parker D., Barbara J. A., Contreras M., Aloysius S. Detection of hepatitis C viral sequences in blood donations by "nested" polymerase chain reaction and prediction of infectivity. Lancet. 1990 Jun 16;335(8703):1419–1422. doi: 10.1016/0140-6736(90)91446-h. [DOI] [PubMed] [Google Scholar]
  9. Gretch D., Lee W., Corey L. Use of aminotransferase, hepatitis C antibody, and hepatitis C polymerase chain reaction RNA assays to establish the diagnosis of hepatitis C virus infection in a diagnostic virology laboratory. J Clin Microbiol. 1992 Aug;30(8):2145–2149. doi: 10.1128/jcm.30.8.2145-2149.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Indraccolo S., Zamarchi R., Veronese M. L., Mazza M. R., Mion M., Veronesi A., Panozzo M., Colombatti M., Barelli A., Rocchetto P. Standardization of in vitro synthesis and detection of HIV-1-specific antibodies. J Immunol Methods. 1993 Jan 4;157(1-2):105–115. doi: 10.1016/0022-1759(93)90076-j. [DOI] [PubMed] [Google Scholar]
  11. Lazizi Y., Elfassi E., Pillot J. Detection of hepatitis C virus sequences in sera with controversial serology by nested polymerase chain reaction. J Clin Microbiol. 1992 Apr;30(4):931–934. doi: 10.1128/jcm.30.4.931-934.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Löhr H., Fleischer B., Michel G., Rossol S., Hess G., Meyer zum Büschenfelde K. H., Manns M. Hepatitis C virus antibody secretion in vitro by peripheral blood lymphocytes. J Hepatol. 1992 Jan;14(1):112–117. doi: 10.1016/0168-8278(92)90139-g. [DOI] [PubMed] [Google Scholar]
  13. Pawlotsky J. M., Fleury A., Choukroun V., Deforges L., Roudot-Thoraval F., Aumont P., Duval J., Dhumeaux D. Significance of highly positive c22-3 "indeterminate" second-generation hepatitis C virus (HCV) recombinant immunoblot assay (RIBA) and resolution by third-generation HCV RIBA. J Clin Microbiol. 1994 May;32(5):1357–1359. doi: 10.1128/jcm.32.5.1357-1359.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Segondy M., Vendrell J. P., Reynes J., Huguet M. F., Albat B., Ducos J., Nicolas J. C., Serre A. Cytomegalovirus-specific B cell activation as a potential marker for the diagnosis of cytomegalovirus infection. Eur J Clin Microbiol Infect Dis. 1990 Oct;9(10):745–750. doi: 10.1007/BF02184687. [DOI] [PubMed] [Google Scholar]
  15. Uyttendaele S., Claeys H., Mertens W., Verhaert H., Vermylen C. Evaluation of third-generation screening and confirmatory assays for HCV antibodies. Vox Sang. 1994;66(2):122–129. doi: 10.1111/j.1423-0410.1994.tb00293.x. [DOI] [PubMed] [Google Scholar]
  16. Vendrell J. P., Segondy M., Ducos J., Reynes J., Huguet M. F., Nicolas J. C., Serre A. Analysis of the spontaneous in vitro anti-HIV-1 antibody secretion by peripheral blood mononuclear cells in HIV-1 infection. Clin Exp Immunol. 1991 Feb;83(2):197–202. doi: 10.1111/j.1365-2249.1991.tb05614.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Vernelen K., Claeys H., Verhaert H., Volckaerts A., Vermylen C. Significance of NS3 and NS5 antigens in screening for HCV antibody. Lancet. 1994 Apr 2;343(8901):853–853. doi: 10.1016/s0140-6736(94)92054-0. [DOI] [PubMed] [Google Scholar]
  18. da Silva Cardoso M., Koerner K., Epple S., Kubanek B. Prevalence of HCV-RNA-positive blood donors and correlation to ELISA and RIBA status. Ann Hematol. 1993 Mar;66(3):147–151. doi: 10.1007/BF01697626. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Clinical Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES