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. 1994 Nov;32(11):2786–2790. doi: 10.1128/jcm.32.11.2786-2790.1994

Disk diffusion versus broth microdilution susceptibility testing of Haemophilus species and Moraxella catarrhalis using seven oral antimicrobial agents: application of updated susceptibility guidelines of the National Committee for Clinical Laboratory Standards.

P C Kibsey 1, R P Rennie 1, J E Rushton 1
PMCID: PMC264160  PMID: 7852573

Abstract

Susceptibility testing of Haemophilus species and Moraxella catarrhalis is medium and inoculum dependent. Seven oral agents, ampicillin, amoxicillin-clavulanic acid, cefaclor, loracarbef, cefuroxime-axetil, cefixime, and erythromycin, were tested against 400 beta-lactamase-positive and -negative clinically significant respiratory strains of Haemophilus species and 100 strains of M. catarrhalis. Sources of the strains included teaching and regional hospitals and a private laboratory. All strains were tested by broth microdilution and disk diffusion in haemophilus test medium for Haemophilus species and Mueller-Hinton broth and agar for M. catarrhalis. Appropriate National Committee for Clinical Laboratory Standards (NCCLS) standards were followed. For Haemophilus species, by disk diffusion and broth microdilution, respectively, 27 and 27% of strains were resistant to ampicillin, 37 and 5% were resistant to erythromycin, 3 and 0.5% were resistant to cefaclor, 2 and 0.5% were resistant to loracarbef, and 0% were resistant to cefuroxime-axetil, cefixime, and amoxicillin-clavulanic acid. beta-Lactamase-negative ampicillin-resistant strains were not observed. Of M. catarrhalis strains, 56% were resistant to ampicillin by disk diffusion and 95% were resistant by broth microdilution. This species was susceptible to all other agents tested by either method. The disagreements between disk diffusion results and MICs for cefaclor, ampicillin, cefuroxime, and loracarbef that occurred with use of the 1990 NCCLS tables were resolved when the 1992 NCCLS tables were used.

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Selected References

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  1. Barry A. L., Fuchs P. C., Pfaller M. A. Susceptibilities of beta-lactamase-producing and -nonproducing ampicillin-resistant strains of Haemophilus influenzae to ceftibuten, cefaclor, cefuroxime, cefixime, cefotaxime, and amoxicillin-clavulanic acid. Antimicrob Agents Chemother. 1993 Jan;37(1):14–18. doi: 10.1128/aac.37.1.14. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Barry A. L., Jorgensen J. H., Hardy D. J. Reproducibility of disc susceptibility tests with Haemophilus influenzae. J Antimicrob Chemother. 1991 Mar;27(3):295–301. doi: 10.1093/jac/27.3.295. [DOI] [PubMed] [Google Scholar]
  3. Bergeron M. G., Simard P., Provencher P. Influence of growth medium and supplement on growth of Haemophilus influenzae and on antibacterial activity of several antibiotics. J Clin Microbiol. 1987 Apr;25(4):650–655. doi: 10.1128/jcm.25.4.650-655.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Catlin B. W. Branhamella catarrhalis: an organism gaining respect as a pathogen. Clin Microbiol Rev. 1990 Oct;3(4):293–320. doi: 10.1128/cmr.3.4.293. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Doern G. V. In vitro susceptibility testing of Haemophilus influenzae: review of new National Committee for Clinical Laboratory Standards recommendations. J Clin Microbiol. 1992 Dec;30(12):3035–3038. doi: 10.1128/jcm.30.12.3035-3038.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Doern G. V., Jorgensen J. H., Thornsberry C., Snapper H. Disk diffusion susceptibility testing of Haemophilus influenzae using haemophilus test medium. Eur J Clin Microbiol Infect Dis. 1990 May;9(5):329–336. doi: 10.1007/BF01973739. [DOI] [PubMed] [Google Scholar]
  7. Doern G. V., Tubert T. A. In vitro activities of 39 antimicrobial agents for Branhamella catarrhalis and comparison of results with different quantitative susceptibility test methods. Antimicrob Agents Chemother. 1988 Feb;32(2):259–261. doi: 10.1128/aac.32.2.259. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Doern G. V., Tubert T. Disk diffusion susceptibility testing of Branhamella catarrhalis with ampicillin and seven other antimicrobial agents. Antimicrob Agents Chemother. 1987 Oct;31(10):1519–1523. doi: 10.1128/aac.31.10.1519. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Heelan J. S., Chesney D., Guadagno G. Investigation of ampicillin-intermediate strains of Haemophilus influenzae by using the disk diffusion procedure and current National Committee for Clinical Laboratory Standards guidelines. J Clin Microbiol. 1992 Jul;30(7):1674–1677. doi: 10.1128/jcm.30.7.1674-1677.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Jorgensen J. H., Doern G. V., Maher L. A., Howell A. W., Redding J. S. Antimicrobial resistance among respiratory isolates of Haemophilus influenzae, Moraxella catarrhalis, and Streptococcus pneumoniae in the United States. Antimicrob Agents Chemother. 1990 Nov;34(11):2075–2080. doi: 10.1128/aac.34.11.2075. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Jorgensen J. H., Redding J. S., Maher L. A., Howell A. W. Improved medium for antimicrobial susceptibility testing of Haemophilus influenzae. J Clin Microbiol. 1987 Nov;25(11):2105–2113. doi: 10.1128/jcm.25.11.2105-2113.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Jorgensen J. H. Update on mechanisms and prevalence of antimicrobial resistance in Haemophilus influenzae. Clin Infect Dis. 1992 May;14(5):1119–1123. doi: 10.1093/clinids/14.5.1119. [DOI] [PubMed] [Google Scholar]
  13. Mendelman P. M., Wiley E. A., Stull T. L., Clausen C., Chaffin D. O., Onay O. Problems with current recommendations for susceptibility testing of Haemophilus influenzae. Antimicrob Agents Chemother. 1990 Aug;34(8):1480–1484. doi: 10.1128/aac.34.8.1480. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Scriver S. R., Low D. E., Simor A. E., Toye B., McGeer A., Jaeger R. Broth microdilution testing of Haemophilus influenzae with haemophilus test medium versus lysed horse blood broth. Canadian Haemophilus Study Group. J Clin Microbiol. 1992 Sep;30(9):2284–2289. doi: 10.1128/jcm.30.9.2284-2289.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Tremblay L. D., L'Ecuyer J., Provencher P., Bergeron M. G. Susceptibility of Haemophilus influenzae to antimicrobial agents used in Canada. Canadian Study Group. CMAJ. 1990 Nov 1;143(9):895–901. [PMC free article] [PubMed] [Google Scholar]

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