Abstract
A set of three bacteriophages (phi K5, phi K20, and phi K95) was used to discriminate between Escherichia coli K5 and K95 strains isolated from patients with urinary tract infections. All the strains tested were detected primarily by phi K5 and thought to carry the capsular antigen K5. Ten O2, 33 O6, and 32 O18 strains proved to be sensitive to the three phages. Eleven of 44 O75:H- strains, all 8 O75:H5 strains, 1 O17 strain, and 1 OR strain were not lysed by phage phi K20, the only phage without activity toward the K95 test strain. It has been suggested that the phi K20-resistant strains carry K95. These findings were confirmed by countercurrent immunoelectrophoresis with K-antigen extracts and anti-K95 serum. These preliminary data indicate the usefulness of these phages to detect K95 strains among K5 isolates.
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- Altmann F., Kwiatkowski B., Stirm S., März L., Unger F. M. A bacteriophage-associated glycanase cleaving beta-pyranosidic linkages of 3-deoxy-D-manno-2-octulosonic acid (KDO). Biochem Biophys Res Commun. 1986 Apr 14;136(1):329–335. doi: 10.1016/0006-291x(86)90914-9. [DOI] [PubMed] [Google Scholar]
- Altmann F., März L., Stirm S., Unger F. M. Two additional bacteriophage-associated glycan hydrolases cleaving ketosidic bonds of 3-deoxy-D-manno-octulosonic acid in capsular polysaccharides of Escherichia coli. FEBS Lett. 1987 Aug 31;221(1):145–149. doi: 10.1016/0014-5793(87)80369-1. [DOI] [PubMed] [Google Scholar]
- Dengler T., Jann B., Jann K. Structure of the K95 antigen from Escherichia coli O75:K95:H5, a capsular polysaccharide containing furanosidic KDO-residues. Carbohydr Res. 1985 Oct 15;142(2):269–276. doi: 10.1016/0008-6215(85)85028-x. [DOI] [PubMed] [Google Scholar]
- Devine D. A., Robinson L., Roberts A. P. Occurrence of K1, K5 and O antigens in Escherichia coli isolates from patients with urinary tract infections or bacteraemia. J Med Microbiol. 1989 Dec;30(4):295–299. doi: 10.1099/00222615-30-4-295. [DOI] [PubMed] [Google Scholar]
- Kaijser B., Jodal U. Escherichia coli K5 antigen in relation to various infections and in healthy individuals. J Clin Microbiol. 1984 Feb;19(2):264–266. doi: 10.1128/jcm.19.2.264-266.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Nimmich W., Schmidt G., Krallmann-Wenzel U. Two different Escherichia coli capsular polysaccharide depolymerases each associated with one of the coliphage phi K5 and phi K20. FEMS Microbiol Lett. 1991 Aug 1;66(2):137–141. doi: 10.1016/0378-1097(91)90322-2. [DOI] [PubMed] [Google Scholar]
- Nimmich W., Zingler G., Falkenhagen U., Naumann G., Bohnenstengel C. Vorkommen von Escherichia-coli-K1- und -K5-Stämmen bei Patienten mit Harnwegsinfektionen. Immun Infekt. 1985 Feb;13(1):15–20. [PubMed] [Google Scholar]
- Nimmich W., Zingler G., Falkenhagen U., Naumann G., Kontny I. O- und H-Antigene von Escherichia coli-K5-Isolaten aus Urin. Z Urol Nephrol. 1988 Apr;81(4):257–261. [PubMed] [Google Scholar]
- Nimmich W., Zingler G., Falkenhagen U., Naumann G., Schulz S., Wachtel D. Schnelltypisierung von Escherichia coli-K-Antigenen mit Hilfe von Bakteriophagen. Z Gesamte Hyg. 1989 Oct;35(10):583–585. [PubMed] [Google Scholar]
- Orskov I., Orskov F. Escherichia coli in extra-intestinal infections. J Hyg (Lond) 1985 Dec;95(3):551–575. doi: 10.1017/s0022172400060678. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Pere A., Selander R. K., Korhonen T. K. Characterization of P fimbriae on O1, O7, O75, rough, and nontypable strains of Escherichia coli. Infect Immun. 1988 May;56(5):1288–1294. doi: 10.1128/iai.56.5.1288-1294.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Sandberg T., Kaijser B., Lidin-Janson G., Lincoln K., Orskov F., Orskov I., Stokland E., Svanborg-Edén C. Virulence of Escherichia coli in relation to host factors in women with symptomatic urinary tract infection. J Clin Microbiol. 1988 Aug;26(8):1471–1476. doi: 10.1128/jcm.26.8.1471-1476.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Semjen G., Orskov I., Orskov F. K antigen determination of Escherichia coli by counter-current immunoelectrophoresis (CIE). Acta Pathol Microbiol Scand B. 1977 Feb;85B(1):103–107. doi: 10.1111/j.1699-0463.1977.tb01680.x. [DOI] [PubMed] [Google Scholar]
- Vann W. F., Schmidt M. A., Jann B., Jann K. The structure of the capsular polysaccharide (K5 antigen) of urinary-tract-infective Escherichia coli 010:K5:H4. A polymer similar to desulfo-heparin. Eur J Biochem. 1981 May 15;116(2):359–364. doi: 10.1111/j.1432-1033.1981.tb05343.x. [DOI] [PubMed] [Google Scholar]
- Vann W. F., Soderstrom T., Egan W., Tsui F. P., Schneerson R., Orskov I., Orskov F. Serological, chemical, and structural analyses of the Escherichia coli cross-reactive capsular polysaccharides K13, K20, and K23. Infect Immun. 1983 Feb;39(2):623–629. doi: 10.1128/iai.39.2.623-629.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Väisänen-Rhen V. Fimbria-like hemagglutinin of Escherichia coli O75 strains. Infect Immun. 1984 Nov;46(2):401–407. doi: 10.1128/iai.46.2.401-407.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]