Skip to main content
NCBI home page
Journal of Clinical Microbiology logoLink to Journal of Clinical Microbiology
. 1994 Nov;32(11):2861–2864. doi: 10.1128/jcm.32.11.2861-2864.1994

Validation of respiratory syncytial virus enzyme immunoassay and shell vial assay results.

L Pedneault 1, L Robillard 1, J P Turgeon 1
PMCID: PMC264177  PMID: 7852589

Abstract

The Pathfinder respiratory syncytial virus (RSV) enzyme immunoassay (EIA) (Kallestad), the shell vial (SV) technique, and conventional cell culture (CC) were compared for detection of RSV in nasopharyngeal aspirates. We found sensitivities, specificities, and positive and negative predictive values of 58.4, 100, 100, and 68.2%; 80.7, 97.2, 97.0, and 81.9%; and 77.6, 97.2, 96.9, and 79.5% for the CC, EIA, and SV methods, respectively. The SV and EIA techniques were both more sensitive than CC (P < 0.001). Finally, 29 respiratory viruses other than RSV were identified by CC.

Full text

PDF
2861

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Ahluwalia G. S., Hammond G. W. Comparison of cell culture and three enzyme-linked immunosorbent assays for the rapid diagnosis of respiratory syncytial virus from nasopharyngeal aspirate and tracheal secretion specimens. Diagn Microbiol Infect Dis. 1988 Mar;9(3):187–192. doi: 10.1016/0732-8893(88)90028-4. [DOI] [PubMed] [Google Scholar]
  2. Arens M. Q., Swierkosz E. M., Schmidt R. R., Armstrong T., Rivetna K. A. Enhanced isolation of respiratory syncytial virus in cell culture. J Clin Microbiol. 1986 Apr;23(4):800–802. doi: 10.1128/jcm.23.4.800-802.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Bromberg K., Tannis G., Daidone B., Clarke L., Sierra M. Comparison of HEp-2 cell culture and Abbott respiratory syncytial virus enzyme immunoassay. J Clin Microbiol. 1987 Feb;25(2):434–436. doi: 10.1128/jcm.25.2.434-436.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Halstead D. C., Todd S., Fritch G. Evaluation of five methods for respiratory syncytial virus detection. J Clin Microbiol. 1990 May;28(5):1021–1025. doi: 10.1128/jcm.28.5.1021-1025.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Hornsleth A., Brenøe E., Friis B., Knudsen F. U., Uldall P. Detection of respiratory syncytial virus in nasopharyngeal secretions by inhibition of enzyme-linked immunosorbent assay. J Clin Microbiol. 1981 Nov;14(5):510–515. doi: 10.1128/jcm.14.5.510-515.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Hughes J. H., Mann D. R., Hamparian V. V. Detection of respiratory syncytial virus in clinical specimens by viral culture, direct and indirect immunofluorescence, and enzyme immunoassay. J Clin Microbiol. 1988 Mar;26(3):588–591. doi: 10.1128/jcm.26.3.588-591.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Johnston S. L., Siegel C. S. Evaluation of direct immunofluorescence, enzyme immunoassay, centrifugation culture, and conventional culture for the detection of respiratory syncytial virus. J Clin Microbiol. 1990 Nov;28(11):2394–2397. doi: 10.1128/jcm.28.11.2394-2397.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Kellogg J. A. Culture vs direct antigen assays for detection of microbial pathogens from lower respiratory tract specimens suspected of containing the respiratory syncytial virus. Arch Pathol Lab Med. 1991 May;115(5):451–458. [PubMed] [Google Scholar]
  9. Lauer B. A., Masters H. A., Wren C. G., Levin M. J. Rapid detection of respiratory syncytial virus in nasopharyngeal secretions by enzyme-linked immunosorbent assay. J Clin Microbiol. 1985 Nov;22(5):782–785. doi: 10.1128/jcm.22.5.782-785.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. McIntosh K., Hendry R. M., Fahnestock M. L., Pierik L. T. Enzyme-linked immunosorbent assay for detection of respiratory syncytial virus infection: application to clinical samples. J Clin Microbiol. 1982 Aug;16(2):329–333. doi: 10.1128/jcm.16.2.329-333.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Rabalais G. P., Stout G. G., Ladd K. L., Cost K. M. Rapid diagnosis of respiratory viral infections by using a shell vial assay and monoclonal antibody pool. J Clin Microbiol. 1992 Jun;30(6):1505–1508. doi: 10.1128/jcm.30.6.1505-1508.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Smith M. C., Creutz C., Huang Y. T. Detection of respiratory syncytial virus in nasopharyngeal secretions by shell vial technique. J Clin Microbiol. 1991 Mar;29(3):463–465. doi: 10.1128/jcm.29.3.463-465.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Subbarao E. K., Whitehurst N. J., Waner J. L. Comparison of two enzyme-linked immunosorbent assay (EIA) kits with immunofluorescence and isolation in cell culture for detection of respiratory syncytial virus (RSV). Diagn Microbiol Infect Dis. 1987 Dec;8(4):229–234. doi: 10.1016/0732-8893(87)90054-x. [DOI] [PubMed] [Google Scholar]
  14. Swenson P. D., Kaplan M. H. Rapid detection of respiratory syncytial virus in nasopharyngeal aspirates by a commercial enzyme immunoassay. J Clin Microbiol. 1986 Mar;23(3):485–488. doi: 10.1128/jcm.23.3.485-488.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Swierkosz E. M., Flanders R., Melvin L., Miller J. D., Kline M. W. Evaluation of the Abbott TESTPACK RSV enzyme immunoassay for detection of respiratory syncytial virus in nasopharyngeal swab specimens. J Clin Microbiol. 1989 Jun;27(6):1151–1154. doi: 10.1128/jcm.27.6.1151-1154.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Clinical Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES