Skip to main content
Journal of Clinical Microbiology logoLink to Journal of Clinical Microbiology
. 1994 Dec;32(12):3052–3053. doi: 10.1128/jcm.32.12.3052-3053.1994

Preliminary results of Pneumocystis carinii strain differentiation by using molecular biology.

S Latouche 1, P Roux 1, J L Poirot 1, I Lavrard 1, B Hermelin 1, V Bertrand 1
PMCID: PMC264226  PMID: 7883899

Abstract

The mode of Pneumocystis carinii transmission is controversial. Recent studies point to exogenous inoculation rather than reactivation, and person-to-person transmission has also been suggested. Comparison of nucleotide sequences of the large-subunit mitochondrial rRNA gene of P. carinii from human immunodeficiency virus-seropositive patients showed strain differences.

Full text

PDF
3052

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Borensztein L., Hatin I., Simonpoli A. M., Ugarte E., Girard P. M., Jaureguiberry G. An alternative to DNA extraction for the diagnosis of Pneumocystis carinii pneumonia by polymerase chain reaction using a new oligonucleotide probe. Mol Cell Probes. 1992 Oct;6(5):361–365. doi: 10.1016/0890-8508(92)90028-v. [DOI] [PubMed] [Google Scholar]
  2. Chave J. P., David S., Wauters J. P., Van Melle G., Francioli P. Transmission of Pneumocystis carinii from AIDS patients to other immunosuppressed patients: a cluster of Pneumocystis carinii pneumonia in renal transplant recipients. AIDS. 1991 Aug;5(8):927–932. doi: 10.1097/00002030-199108000-00002. [DOI] [PubMed] [Google Scholar]
  3. Cushion M. T., Zhang J., Kaselis M., Giuntoli D., Stringer S. L., Stringer J. R. Evidence for two genetic variants of Pneumocystis carinii coinfecting laboratory rats. J Clin Microbiol. 1993 May;31(5):1217–1223. doi: 10.1128/jcm.31.5.1217-1223.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Glotz D., Kazatchkine M., Vincent F., Haddad H., Duboust A., Druet P., Bariety J. Pneumocystoses dans un centre de transplantation rénale proche d'une unité de soins de malades sidéens. Presse Med. 1992 Dec 19;21(44):2154–2154. [PubMed] [Google Scholar]
  5. Goesch T. R., Götz G., Stellbrinck K. H., Albrecht H., Weh H. J., Hossfeld D. K. Possible transfer of Pneumocystis carinii between immunodeficient patients. Lancet. 1990 Sep 8;336(8715):627–627. doi: 10.1016/0140-6736(90)93420-t. [DOI] [PubMed] [Google Scholar]
  6. Hong S. T., Steele P. E., Cushion M. T., Walzer P. D., Stringer S. L., Stringer J. R. Pneumocystis carinii karyotypes. J Clin Microbiol. 1990 Aug;28(8):1785–1795. doi: 10.1128/jcm.28.8.1785-1795.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Kovacs J. A., Halpern J. L., Lundgren B., Swan J. C., Parrillo J. E., Masur H. Monoclonal antibodies to Pneumocystis carinii: identification of specific antigens and characterization of antigenic differences between rat and human isolates. J Infect Dis. 1989 Jan;159(1):60–70. doi: 10.1093/infdis/159.1.60. [DOI] [PubMed] [Google Scholar]
  8. Lee C. H., Lu J. J., Bartlett M. S., Durkin M. M., Liu T. H., Wang J., Jiang B., Smith J. W. Nucleotide sequence variation in Pneumocystis carinii strains that infect humans. J Clin Microbiol. 1993 Mar;31(3):754–757. doi: 10.1128/jcm.31.3.754-757.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Lipschik G. Y., Gill V. J., Lundgren J. D., Andrawis V. A., Nelson N. A., Nielsen J. O., Ognibene F. P., Kovacs J. A. Improved diagnosis of Pneumocystis carinii infection by polymerase chain reaction on induced sputum and blood. Lancet. 1992 Jul 25;340(8813):203–206. doi: 10.1016/0140-6736(92)90469-j. [DOI] [PubMed] [Google Scholar]
  10. Lundgren B., Cotton R., Lundgren J. D., Edman J. C., Kovacs J. A. Identification of Pneumocystis carinii chromosomes and mapping of five genes. Infect Immun. 1990 Jun;58(6):1705–1710. doi: 10.1128/iai.58.6.1705-1710.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Sinclair K., Wakefield A. E., Banerji S., Hopkin J. M. Pneumocystis carinii organisms derived from rat and human hosts are genetically distinct. Mol Biochem Parasitol. 1991 Mar;45(1):183–184. doi: 10.1016/0166-6851(91)90042-5. [DOI] [PubMed] [Google Scholar]
  12. Stringer J. R., Stringer S. L., Zhang J., Baughman R., Smulian A. G., Cushion M. T. Molecular genetic distinction of Pneumocystis carinii from rats and humans. J Eukaryot Microbiol. 1993 Nov-Dec;40(6):733–741. doi: 10.1111/j.1550-7408.1993.tb04468.x. [DOI] [PubMed] [Google Scholar]
  13. Wada M., Kitada K., Saito M., Egawa K., Nakamura Y. cDNA sequence diversity and genomic clusters of major surface glycoprotein genes of Pneumocystis carinii. J Infect Dis. 1993 Oct;168(4):979–985. doi: 10.1093/infdis/168.4.979. [DOI] [PubMed] [Google Scholar]
  14. Wakefield A. E., Pixley F. J., Banerji S., Sinclair K., Miller R. F., Moxon E. R., Hopkin J. M. Detection of Pneumocystis carinii with DNA amplification. Lancet. 1990 Aug 25;336(8713):451–453. doi: 10.1016/0140-6736(90)92008-6. [DOI] [PubMed] [Google Scholar]
  15. Walzer P. D., Linke M. J. A comparison of the antigenic characteristics of rat and human Pneumocystis carinii by immunoblotting. J Immunol. 1987 Apr 1;138(7):2257–2265. [PubMed] [Google Scholar]

Articles from Journal of Clinical Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES