Abstract
The mip gene of Legionella pneumophila was demonstrated by PCR and probing in paired acute-phase and convalescent-phase sera from five patients with Legionnaires' disease but not in the acute-phase sera of 100 patients with pneumonia that showed no serological evidence of Legionella infection. PCR may help in cases presenting diagnostic difficulty.
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- Birtles R. J., Harrison T. G., Samuel D., Taylor A. G. Evaluation of urinary antigen ELISA for diagnosing Legionella pneumophila serogroup 1 infection. J Clin Pathol. 1990 Aug;43(8):685–690. doi: 10.1136/jcp.43.8.685. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Cianciotto N. P., Eisenstein B. I., Mody C. H., Engleberg N. C. A mutation in the mip gene results in an attenuation of Legionella pneumophila virulence. J Infect Dis. 1990 Jul;162(1):121–126. doi: 10.1093/infdis/162.1.121. [DOI] [PubMed] [Google Scholar]
- Cianciotto N. P., Eisenstein B. I., Mody C. H., Toews G. B., Engleberg N. C. A Legionella pneumophila gene encoding a species-specific surface protein potentiates initiation of intracellular infection. Infect Immun. 1989 Apr;57(4):1255–1262. doi: 10.1128/iai.57.4.1255-1262.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Engleberg N. C., Carter C., Weber D. R., Cianciotto N. P., Eisenstein B. I. DNA sequence of mip, a Legionella pneumophila gene associated with macrophage infectivity. Infect Immun. 1989 Apr;57(4):1263–1270. doi: 10.1128/iai.57.4.1263-1270.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Horwitz M. A., Silverstein S. C. Legionnaires' disease bacterium (Legionella pneumophila) multiples intracellularly in human monocytes. J Clin Invest. 1980 Sep;66(3):441–450. doi: 10.1172/JCI109874. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Jaulhac B., Nowicki M., Bornstein N., Meunier O., Prevost G., Piemont Y., Fleurette J., Monteil H. Detection of Legionella spp. in bronchoalveolar lavage fluids by DNA amplification. J Clin Microbiol. 1992 Apr;30(4):920–924. doi: 10.1128/jcm.30.4.920-924.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kolk A. H., Schuitema A. R., Kuijper S., van Leeuwen J., Hermans P. W., van Embden J. D., Hartskeerl R. A. Detection of Mycobacterium tuberculosis in clinical samples by using polymerase chain reaction and a nonradioactive detection system. J Clin Microbiol. 1992 Oct;30(10):2567–2575. doi: 10.1128/jcm.30.10.2567-2575.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Mahbubani M. H., Bej A. K., Miller R., Haff L., DiCesare J., Atlas R. M. Detection of Legionella with polymerase chain reaction and gene probe methods. Mol Cell Probes. 1990 Jun;4(3):175–187. doi: 10.1016/0890-8508(90)90051-z. [DOI] [PubMed] [Google Scholar]
- Rudolph K. M., Parkinson A. J., Black C. M., Mayer L. W. Evaluation of polymerase chain reaction for diagnosis of pneumococcal pneumonia. J Clin Microbiol. 1993 Oct;31(10):2661–2666. doi: 10.1128/jcm.31.10.2661-2666.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Schluger N. W., Condos R., Lewis S., Rom W. N. Amplification of DNA of Mycobacterium tuberculosis from peripheral blood of patients with pulmonary tuberculosis. Lancet. 1994 Jul 23;344(8917):232–233. doi: 10.1016/s0140-6736(94)92999-8. [DOI] [PubMed] [Google Scholar]
- Wilkinson H. W., Cruce D. D., Broome C. V. Validation of Legionella pneumophila indirect immunofluorescence assay with epidemic sera. J Clin Microbiol. 1981 Jan;13(1):139–146. doi: 10.1128/jcm.13.1.139-146.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Wolcott M. J. Advances in nucleic acid-based detection methods. Clin Microbiol Rev. 1992 Oct;5(4):370–386. doi: 10.1128/cmr.5.4.370. [DOI] [PMC free article] [PubMed] [Google Scholar]