Abstract
The transport medium Multi-Microbe Media (M4) was evaluated prospectively by culture and direct enzyme-linked immunosorbent assay (ELISA) for detection of herpes simplex virus from 473 specimens. In addition, 377 specimens in Bartels Viral Transport Medium were evaluated. By using culture as a "gold standard," the ELISA sensitivity was approximately 85%, while the specificities exceeded 96% for both media.
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Selected References
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- Baker D. A., Gonik B., Milch P. O., Berkowitz A., Lipson S., Verma U. Clinical evaluation of a new herpes simplex virus ELISA: a rapid diagnostic test for herpes simplex virus. Obstet Gynecol. 1989 Mar;73(3 Pt 1):322–325. [PubMed] [Google Scholar]
- Dascal A., Chan-Thim J., Morahan M., Portnoy J., Mendelson J. Replacement of special enzyme immunoassay transport medium by a standard viral transport medium in the Herpcheck herpes simplex virus antigen detection test. Diagn Microbiol Infect Dis. 1989 Nov-Dec;12(6):473–475. doi: 10.1016/0732-8893(89)90080-1. [DOI] [PubMed] [Google Scholar]
- Dunkel E. C., Pavan-Langston D., Fitzpatrick K., Cukor G. Rapid detection of herpes simplex virus (HSV) antigen in human ocular infections. Curr Eye Res. 1988 Jul;7(7):661–666. doi: 10.3109/02713688809033194. [DOI] [PubMed] [Google Scholar]
- Gleaves C. A., Rice D. H., Lee C. F. Evaluation of an enzyme immunoassay for the detection of herpes simplex virus (HSV) antigen from clinical specimens in viral transport media. J Virol Methods. 1990 May;28(2):133–139. doi: 10.1016/0166-0934(90)90027-d. [DOI] [PubMed] [Google Scholar]
- Moseley R. C., Corey L., Benjamin D., Winter C., Remington M. L. Comparison of viral isolation, direct immunofluorescence, and indirect immunoperoxidase techniques for detection of genital herpes simplex virus infection. J Clin Microbiol. 1981 May;13(5):913–918. doi: 10.1128/jcm.13.5.913-918.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Oefinger P. E., Shawar R. M., Loo S. H., Tsai L. T., Arnett J. K. Enhanced recovery of cytomegalovirus in conventional tube cultures with a spin-amplified adsorption. J Clin Microbiol. 1990 May;28(5):965–969. doi: 10.1128/jcm.28.5.965-969.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Verano L., Michalski F. J. Herpes simplex virus antigen direct detection in standard virus transport medium by Du Pont Herpchek enzyme-linked immunosorbent assay. J Clin Microbiol. 1990 Nov;28(11):2555–2558. doi: 10.1128/jcm.28.11.2555-2558.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Wu T. C., Zaza S., Callaway J. Evaluation of the Du Pont HERPCHEK herpes simplex virus antigen test with clinical specimens. J Clin Microbiol. 1989 Aug;27(8):1903–1905. doi: 10.1128/jcm.27.8.1903-1905.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]