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Journal of Clinical Microbiology logoLink to Journal of Clinical Microbiology
. 2009 Feb;47(2):519. doi: 10.1128/JCM.02247-08

Multicenter Comparison of PCR Assays for Detection of Human Herpesvirus 6 DNA in Serum

Louis Flamand 1, Annie Gravel 1, David Boutolleau 1, Roberto Alvarez-Lafuente 1, Steve Jacobson 1, Mauro S Malnati 1, Debra Kohn 1, Yi-Wei Tang 1, Tetsushi Yoshikawa 1, Dharam Ablashi 1
PMCID: PMC2643693

Volume 46, no. 8, p. 2700-2706, 2008. Page 2701, Table 1, column 3, line 1: “5′-CGAAACGCCTACACAGAAT-3′” should read “5′-CAAAGCCAAATTATCCAGAGCG-3′.”

Page 2701, Table 1, column 3, lines 3 and 4: “5′-CAAAGCCAAATTATCCAGAGCG-3′” should read “5′-CGAAACGCCTACACAGAAT-3′.”

Page 2701, Table 1, column 4, lines 1 and 2: “5′-CAAAGCCAAATTATCCAGAGCG-3′” should read “5′-CGCTAGGTTGAG(G/A)ATGATCGA-3′.”

Page 2701, Table 1, column 4, lines 3 and 4: “5′-CGCTAGGTTGAGGATGATCGA-3′” should read “5′-CAAAGCCAAATTATCCAGAGCG-3′.”

Page 2701, Table 1, column 5, line 1: “5′-CGTCACACCCGAAGGAAT-3′” should read “5′-CCCGAAGGAATAACGCTC-3′.”

Page 2701, Table 1, column 5, line 3: “5′-CCCGAAGGAATAACGCTC-3′” should read “5′-CGTCACACCCGAAGGAAT-3′.”

Note that for assay 1, the second primer was modified to include a G/A at position 13 to account for a polymorphism in the HHV-6B variant. The conclusion that assays 1 and 6 were the most reliable remains unchanged.


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