Skip to main content
NCBI home page
Infection and Immunity logoLink to Infection and Immunity
. 1983 Dec;42(3):863–868. doi: 10.1128/iai.42.3.863-868.1983

Monoclonal antibodies against Neisseria meningitidis lipopolysaccharide.

R J Sugasawara, C Prato, J E Sippel
PMCID: PMC264378  PMID: 6417025

Abstract

A cell line producing monoclonal antibodies directed against a lipopolysaccharide component of Neisseria meningitidis group A has been established. These antibodies reacted with only one of three lipopolysaccharide serotyping strains of group A meningococci by coagglutination, enzyme-linked immunosorbent assay, and Western blotting techniques. A Western blot analysis showed that a NaOH digest of lipopolysaccharide was detectable by the serotype-specific antibody. The monoclonal antibodies cross-reacted with a group B meningococcal strain in an enzyme-linked immunosorbent assay. The immunoblotting analysis also showed that these antibodies reacted with the lipopolysaccharides of a group B meningococcus as well as Haemophilus influenzae type B, but not with the lipopolysaccharides of several strains of Salmonella typhi, Escherichia coli, Streptococcus pneumoniae, and Neisseria gonorrhoeae.

Full text

PDF
863

Images in this article

865Image
on p.865
867Image
on p.867

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Apicella M. A., Bennett K. M., Hermerath C. A., Roberts D. E. Monoclonal antibody analysis of lipopolysaccharide from Neisseria gonorrhoeae and Neisseria meningitidis. Infect Immun. 1981 Dec;34(3):751–756. doi: 10.1128/iai.34.3.751-756.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Apicella M. A. Lipopolysaccharide-derived serotype polysaccharides from Neisseria meningitidis group B. J Infect Dis. 1979 Jul;140(1):62–72. doi: 10.1093/infdis/140.1.62. [DOI] [PubMed] [Google Scholar]
  3. Burnette W. N. "Western blotting": electrophoretic transfer of proteins from sodium dodecyl sulfate--polyacrylamide gels to unmodified nitrocellulose and radiographic detection with antibody and radioiodinated protein A. Anal Biochem. 1981 Apr;112(2):195–203. doi: 10.1016/0003-2697(81)90281-5. [DOI] [PubMed] [Google Scholar]
  4. Edwards E. A., Coonrod J. D. Coagglutination and counterimmunoelectrophoresis for detection of pneumococcal antigens in the sputum of pneumonia patients. J Clin Microbiol. 1980 May;11(5):488–491. doi: 10.1128/jcm.11.5.488-491.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Engvall E., Perlmann P. Enzyme-linked immunosorbent assay (ELISA). Quantitative assay of immunoglobulin G. Immunochemistry. 1971 Sep;8(9):871–874. doi: 10.1016/0019-2791(71)90454-x. [DOI] [PubMed] [Google Scholar]
  6. Frasch C. E., Gotschlich E. C. An outer membrane protein of Neisseria meningitidis group B responsible for serotype specificity. J Exp Med. 1974 Jul 1;140(1):87–104. doi: 10.1084/jem.140.1.87. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Griffiss J. M. Epidemiological value of lipopolysaccharide and heat-modifiable outer-membrane protein serotyping of group-A strains of Neisseria meningitidis. J Med Microbiol. 1982 Aug;15(3):327–330. doi: 10.1099/00222615-15-3-327. [DOI] [PubMed] [Google Scholar]
  8. Gustafsson B., Rosén A., Holme T. Monoclonal antibodies against Vibrio cholerae lipopolysaccharide. Infect Immun. 1982 Nov;38(2):449–454. doi: 10.1128/iai.38.2.449-454.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. HURWITZ J., WEISSBACH A. The formation of 2-keto-3-deoxyheptonic acid in extracts of Escherichia coli B. II. Enzymic studies. J Biol Chem. 1959 Apr;234(4):710–712. [PubMed] [Google Scholar]
  10. Higashi G. I., Sippel J. E., Girgis N. I., Hassan A. Counterimmunoelectrophoresis: an adjunct to bacterial culture in the diagnosis of meningococcal meningitis. Scand J Infect Dis. 1974;6(3):233–235. doi: 10.3109/inf.1974.6.issue-3.04. [DOI] [PubMed] [Google Scholar]
  11. Iscove N. N., Melchers F. Complete replacement of serum by albumin, transferrin, and soybean lipid in cultures of lipopolysaccharide-reactive B lymphocytes. J Exp Med. 1978 Mar 1;147(3):923–933. doi: 10.1084/jem.147.3.923. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. James J. F., Swanson J. Studies on gonococcus infection. XIII. Occurrence of color/opacity colonial variants in clinical cultures. Infect Immun. 1978 Jan;19(1):332–340. doi: 10.1128/iai.19.1.332-340.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Kennett R. H. Cell fusion. Methods Enzymol. 1979;58:345–359. doi: 10.1016/s0076-6879(79)58149-x. [DOI] [PubMed] [Google Scholar]
  14. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  15. Nachamkin I., Cannon J. G., Mittler R. S. Monoclonal antibodies against Neisseria gonorrhoeae: production of antibodies directed against a strain-specific cell surface antigen. Infect Immun. 1981 May;32(2):641–648. doi: 10.1128/iai.32.2.641-648.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Olcén P. Serological methods for rapid diagnosis of haemophilus influenzae, Neisseria meningitidis and Streptococcus pneumoniae in cerebrospinal fluid: a comparison of Co-agglutination, immunofluorescence and immunoelectroosmophoresis. Scand J Infect Dis. 1978;10(4):283–289. doi: 10.3109/inf.1978.10.issue-4.05. [DOI] [PubMed] [Google Scholar]
  17. Robertson S. M., Frisch C. F., Gulig P. A., Kettman J. R., Johnston K. H., Hansen E. J. Monoclonal antibodies directed against a cell surface-exposed outer membrane protein of Haemophilus influenzae type b. Infect Immun. 1982 Apr;36(1):80–88. doi: 10.1128/iai.36.1.80-88.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Sippel J. E., Girgis N. I. Meningococcal infection in Egypt: laboratory findings in meningitis patients and the prevalence of pharyngeal infection in patients and contacts. Am J Trop Med Hyg. 1978 Sep;27(5):980–985. doi: 10.4269/ajtmh.1978.27.980. [DOI] [PubMed] [Google Scholar]
  19. Sippel J. E., Quan A. Homogeneity of protein serotype antigens in Neisseria meningitidis group A. Infect Immun. 1977 May;16(2):623–627. doi: 10.1128/iai.16.2.623-627.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Sippel J. E., Voller A. Detection of Neisseria meningitidis cell envelope antigen by enzyme-linked immunosorbent assay in patients with meningococcal disease. Trans R Soc Trop Med Hyg. 1980;74(5):644–648. doi: 10.1016/0035-9203(80)90156-x. [DOI] [PubMed] [Google Scholar]
  21. Swanson J., Mayer L. W., Tam M. R. Antigenicity of Neisseria gonorrhoeae outer membrane protein(s) III detected by immunoprecipitation and Western blot transfer with a monoclonal antibody. Infect Immun. 1982 Nov;38(2):668–672. doi: 10.1128/iai.38.2.668-672.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Tam M. R., Buchanan T. M., Sandström E. G., Holmes K. K., Knapp J. S., Siadak A. W., Nowinski R. C. Serological classification of Neisseria gonorrhoeae with monoclonal antibodies. Infect Immun. 1982 Jun;36(3):1042–1053. doi: 10.1128/iai.36.3.1042-1053.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Towbin H., Staehelin T., Gordon J. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci U S A. 1979 Sep;76(9):4350–4354. doi: 10.1073/pnas.76.9.4350. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Tsai C. M., Frasch C. E. A sensitive silver stain for detecting lipopolysaccharides in polyacrylamide gels. Anal Biochem. 1982 Jan 1;119(1):115–119. doi: 10.1016/0003-2697(82)90673-x. [DOI] [PubMed] [Google Scholar]
  25. Zollinger W. D., Mandrell R. E. Outer-membrane protein and lipopolysaccharide serotyping of Neisseria meningitidis by inhibition of a solid-phase radioimmunoassay. Infect Immun. 1977 Nov;18(2):424–433. doi: 10.1128/iai.18.2.424-433.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Zollinger W. D., Mandrell R. E. Type-specific antigens of group A Neisseria meningitidis: lipopolysaccharide and heat-modifiable outer membrane proteins. Infect Immun. 1980 May;28(2):451–458. doi: 10.1128/iai.28.2.451-458.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. de StGroth S. F., Scheidegger D. Production of monoclonal antibodies: strategy and tactics. J Immunol Methods. 1980;35(1-2):1–21. doi: 10.1016/0022-1759(80)90146-5. [DOI] [PubMed] [Google Scholar]

Articles from Infection and Immunity are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES