Abstract
The ability to cause subcutaneous abscesses in mice was used to identify the pathogens among the bacteria recovered from 13 clinical abscesses. A total of 35 isolates (30 anaerobes and 5 aerobes), 16 of which were encapsulated, were recovered from these abscesses. Encapsulated organisms included eight Bacteroides spp. (three Bacteroides asaccharolyticus and one strain each of Bacteroides oralis, Bacteroides intermedius, Bacteroides biacutus, Bacteroides vulgatus, and Bacteroides ruminicola subsp. brevis), three anaerobic gram-positive cocci, two Clostridium spp., and two strains of Escherichia coli. Single organisms, or combinations thereof, obtained from these abscesses were inoculated subcutaneously into mice. All but one of the encapsulated organisms were able to cause abscesses by themselves and were recovered from the abscesses when inoculated with other organisms. Seven non-encapsulated organisms which were also recovered mixed with the encapsulated organisms were never able to cause abscesses nor could they be recovered after they were injected alone. Thirteen strains that did not induce abscesses when injected by themselves into mice survived when injected with other organisms which were encapsulated. In three instances, pairs of non-encapsulated organisms belonging to the last group were able to induce an abscess and survive in it when inoculated together. It was shown that the possession of a capsule by a clinical isolate increases the likelihood that it is a major contributor to the infectious process.
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- Brook I. Bacterial studies of peritoneal cavity and postoperative surgical wound drainage following perforated appendix in children. Ann Surg. 1980 Aug;192(2):208–212. doi: 10.1097/00000658-198008000-00014. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kasper D. L. The polysaccharide capsule of Bacteroides fragilis subspecies fragilis: immunochemical and morphologic definition. J Infect Dis. 1976 Jan;133(1):79–87. doi: 10.1093/infdis/133.1.79. [DOI] [PubMed] [Google Scholar]
- Meleney F. L. BACTERIAL SYNERGISM IN DISEASE PROCESSES: WITH A CONFIRMATION OF THE SYNERGISTIC BACTERIAL ETIOLOGY OF A CERTAIN TYPE OF PROGRESSIVE GANGRENE OF THE ABDOMINAL WALL. Ann Surg. 1931 Dec;94(6):961–981. doi: 10.1097/00000658-193112000-00001. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Okuda K., Takazoe I. Antiphagocytic effects of the capsular structure of a pathogenic strain of Bacteroides melaninogenicus. Bull Tokyo Dent Coll. 1973 Aug;14(3):99–104. [PubMed] [Google Scholar]
- Onderdonk A. B., Bartlett J. G., Louie T., Sullivan-Seigler N., Gorbach S. L. Microbial synergy in experimental intra-abdominal abscess. Infect Immun. 1976 Jan;13(1):22–26. doi: 10.1128/iai.13.1.22-26.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Onderdonk A. B., Kasper D. L., Cisneros R. L., Bartlett J. G. The capsular polysaccharide of Bacteroides fragilis as a virulence factor: comparison of the pathogenic potential of encapsulated and unencapsulated strains. J Infect Dis. 1977 Jul;136(1):82–89. doi: 10.1093/infdis/136.1.82. [DOI] [PubMed] [Google Scholar]
- SOCRANSKY S. S., GIBBONS R. J. REQUIRED ROLE OF BACTEROIDES MELANINOGENICUS IN MIXED ANAEROBIC INFECTIONS. J Infect Dis. 1965 Jun;115:247–253. doi: 10.1093/infdis/115.3.247. [DOI] [PubMed] [Google Scholar]
- Simon G. L., Klempner M. S., Kasper D. L., Gorbach S. L. Alterations in opsonophagocytic killing by neutrophils of Bacteroides fragilis associated with animal and laboratory passage: effect of capsular polysaccharide. J Infect Dis. 1982 Jan;145(1):72–77. doi: 10.1093/infdis/145.1.72. [DOI] [PubMed] [Google Scholar]
- Takazoe I., Tanaka M., Homma T. A pathogenic strain of Bacteroides melaninogenicus. Arch Oral Biol. 1971 Jul;16(7):817–822. doi: 10.1016/0003-9969(71)90126-9. [DOI] [PubMed] [Google Scholar]