Skip to main content
PMC home page
Infection and Immunity logoLink to Infection and Immunity
. 1983 Dec;42(3):1013–1016. doi: 10.1128/iai.42.3.1013-1016.1983

Soluble peptidoglycan from Staphylococcus aureus is a murine B-lymphocyte mitogen.

U M Babu, A R Zeiger
PMCID: PMC264400  PMID: 6605928

Abstract

Soluble peptidoglycan from Staphylococcus aureus has been shown to be capable of causing murine B lymphocytes from the spleen to proliferate and to secrete immunoglobulins in both an in vitro and an in vivo assay. The optimal concentration in vitro was between 33 and 100 micrograms/ml. A 3-day incubation with soluble peptidoglycan was more stimulatory than was a 1- or 2-day incubation. Removal of most of the T lymphocytes with anti-theta serum did not result in any significant change in the mitogenic activity of soluble peptidoglycan on the remaining B cells.

Full text

PDF
1013

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Dziarski R. Polyclonal activation of immunoglobulin secretion in B lymphocytes induced by staphylococcal peptidoglycan. J Immunol. 1980 Dec;125(6):2478–2483. [PubMed] [Google Scholar]
  2. Dziarski R. Preferential induction of autoantibody secretion in polyclonal activation by peptidoglycan and lipopolysaccharide. II. In vivo studies. J Immunol. 1982 Mar;128(3):1026–1030. [PubMed] [Google Scholar]
  3. Dziarski R. Relationships between adjuvant, immunosuppressive, and mitogenic activities of staphylococcal peptidoglycan. Infect Immun. 1979 Nov;26(2):508–514. doi: 10.1128/iai.26.2.508-514.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Dziarski R. Studies on the mechanism of peptidoglycan- and lipopolysaccharide-induced polyclonal activation. Infect Immun. 1982 Feb;35(2):507–514. doi: 10.1128/iai.35.2.507-514.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Gronowicz E., Coutinho A., Melchers F. A plaque assay for all cells secreting Ig of a given type or class. Eur J Immunol. 1976 Aug;6(8):588–590. doi: 10.1002/eji.1830060812. [DOI] [PubMed] [Google Scholar]
  6. Heymer B. Biological properties of the peptidoglycan. Z Immunitatsforsch Exp Klin Immunol. 1975 Jul;149(2-4):245–257. [PubMed] [Google Scholar]
  7. Hrsak I., Tomasić J., Pavelić K., Valinger Z. Stimulation of humoral immunity by peptidoglycan monomer from Brevibacterium divaricatum. Z Immunitatsforsch Immunobiol. 1979 Mar;155(4):312–318. [PubMed] [Google Scholar]
  8. Keglević D., Ladesić B., Hadzija O., Tomasić J., Valinger Z., Pokorny M., Naumski R. Isolation and study of the composition of a peptidoglycan complex excreted by the biotin-requiring mutant of Brevibacterium divaricatum NRRL-2311 in the presence of penicillin. Eur J Biochem. 1974 Mar 1;42(2):389–400. doi: 10.1111/j.1432-1033.1974.tb03351.x. [DOI] [PubMed] [Google Scholar]
  9. Kotani S., Watanabe Y., Shimono T., Narita T., Kato K. Immunoadjuvant activities of cell walls, their water-soluble fractions and peptidoglycan subunits, prepared from various gram-positive bacteria, and of synthetic n-acetylmuramyl peptides. Z Immunitatsforsch Exp Klin Immunol. 1975 Jul;149(2-4):302–319. [PubMed] [Google Scholar]
  10. Mirelman D., Bracha R., Sharon N. Penicillin-induced secretion of soluble, uncross-linked peptidoglycan by Micrococcus luteus cells. Biochemistry. 1974 Nov 19;13(24):5045–5053. doi: 10.1021/bi00721a028. [DOI] [PubMed] [Google Scholar]
  11. Rosenthal R. S., Jungkind D., Daneo-Moore L., Shockman G. D. Evidence for the synthesis of soluble peptidoglycan fragments by protoplasts of Streptococcus faecalis. J Bacteriol. 1975 Oct;124(1):398–409. doi: 10.1128/jb.124.1.398-409.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Rotta J. Endotoxin-like properties of the peptidoglycan. Z Immunitatsforsch Exp Klin Immunol. 1975 Jul;149(2-4):230–244. [PubMed] [Google Scholar]
  13. Stewart-Tull D. E. The immunological activities of bacterial peptidoglycans. Annu Rev Microbiol. 1980;34:311–340. doi: 10.1146/annurev.mi.34.100180.001523. [DOI] [PubMed] [Google Scholar]
  14. Tynecka Z., Ward J. B. Peptidoglycan synthesis in Bacillus licheniformis. The inhibition of cross-linking by benzylpenicillin and cephaloridine in vivo accompanied by the formation of soluble peptidoglycan. Biochem J. 1975 Jan;146(1):253–267. doi: 10.1042/bj1460253. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Wong A. K., Sabbadini E. Plaque assay for the detection of alloantibody-producing cells in mice. J Immunol. 1974 Jun;112(6):2251–2259. [PubMed] [Google Scholar]
  16. Zeiger A. R., Eaton S. M., Mirelman D. Antibodies against a synthetic peptidoglycan-precursor pentapeptide cross-react with at least two distinct populations of uncross-linked soluble peptidoglycan secreted by Micrococcus luteus cells. Eur J Biochem. 1978 May;86(1):235–240. doi: 10.1111/j.1432-1033.1978.tb12304.x. [DOI] [PubMed] [Google Scholar]
  17. Zeiger A. R., Wong W., Chatterjee A. N., Young F. E., Tuazon C. U. Evidence for the secretion of soluble peptidoglycans by clinical isolates of Staphylococcus aureus. Infect Immun. 1982 Sep;37(3):1112–1118. doi: 10.1128/iai.37.3.1112-1118.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Infection and Immunity are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES