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. 1983 Dec;42(3):1020–1026. doi: 10.1128/iai.42.3.1020-1026.1983

Detection of anti-teichoic acid immunoglobulin G antibodies in experimental Staphylococcus epidermidis endocarditis.

T E West, J R Cantey, M A Apicella, N M Burdash
PMCID: PMC264402  PMID: 6642658

Abstract

An enzyme-linked immunosorbent assay (ELISA) was developed for the detection of rabbit immunoglobulin G (IgG) antibodies to purified cell wall teichoic acids from the Staphylococcus aureus Lafferty strain and three strains of coagulase-negative staphylococci. Significant immunological cross-reactivity occurred only between the teichoic acid of S. aureus and one coagulase-negative preparation. The ELISA was used to determine the serum IgG response to Staphylococcus epidermidis in a rabbit model of aortic valve endocarditis. Blood samples were drawn before inoculation and then every 5 days until death or sacrifice at 32 to 35 days postinoculation. Valve vegetations were culture positive at autopsy in 16 (59%) of the 27 catheterized rabbits. Antibody titers in this culture-positive group and the culture-negative group began to rise as early as day 6. Although both groups demonstrated an antibody response, the culture-positive group attained a significantly higher titer on days 26 and 31. Antibodies also rose in a control group of rabbits without a heart catheter but which were inoculated with bacteria. Again, the antibody titer was significantly less than that for the culture-positive group. This ELISA may be useful for the diagnosis of coagulase-negative staphylococcal infections in humans.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Christensen G. D., Bisno A. L., Parisi J. T., McLaughlin B., Hester M. G., Luther R. W. Nosocomial septicemia due to multiply antibiotic-resistant Staphylococcus epidermidis. Ann Intern Med. 1982 Jan;96(1):1–10. doi: 10.7326/0003-4819-96-1-1. [DOI] [PubMed] [Google Scholar]
  2. Crowder J. G., White A. Teichoic acid antibodies in staphylococcal and nonstaphylococcal endocarditis. Ann Intern Med. 1972 Jul;77(1):87–90. doi: 10.7326/0003-4819-77-1-87. [DOI] [PubMed] [Google Scholar]
  3. DAVISON A. L., BADDILEY J. GLYCEROL TEICHOIC ACIDS IN WALLS OF STAPHYLOCOCCUS EPIDERMIDIS. Nature. 1964 May 30;202:874–874. doi: 10.1038/202874a0. [DOI] [PubMed] [Google Scholar]
  4. Downham W. H., Rhoades E. R. Endocarditis associated with porcine valve xenografts. Arch Intern Med. 1979 Dec;139(12):1350–1352. [PubMed] [Google Scholar]
  5. Engvall E., Perlmann P. Enzyme-linked immunosorbent assay, Elisa. 3. Quantitation of specific antibodies by enzyme-labeled anti-immunoglobulin in antigen-coated tubes. J Immunol. 1972 Jul;109(1):129–135. [PubMed] [Google Scholar]
  6. Johnsen G. S., Endresen C., Grov A., Oeding P. Studies on polysaccharide C of Staphylococcus epidermidis. 1. Isolation and chemical characterization. Acta Pathol Microbiol Scand B. 1975 Jun;83(3):226–234. doi: 10.1111/j.1699-0463.1975.tb00096.x. [DOI] [PubMed] [Google Scholar]
  7. Johnsen G. S., Grov A., Oeding P. Studies on polysaccharide C of staphylococcus epidermidis. 2. Antigenic properties. Acta Pathol Microbiol Scand B. 1975 Jun;83(3):235–239. doi: 10.1111/j.1699-0463.1975.tb00097.x. [DOI] [PubMed] [Google Scholar]
  8. LOSNEGARD N., OEDING P. IMMUNOCHEMICAL STUDIES ON POLYSACCHARIDES FROM STAPHYLOCOCCUS EPIDERMIDIS. 1. ISOLATION AND CHEMICAL CHARACTERIZATION. Acta Pathol Microbiol Scand. 1963;58:482–492. doi: 10.1111/j.1699-0463.1963.tb01595.x. [DOI] [PubMed] [Google Scholar]
  9. LOSNEGARD N., OEDING P. IMMUNOCHEMICAL STUDIES ON POLYSACCHARIDES FROM STAPHYLOCOCCUS EPIDERMIDIS. 2. ANTIGENIC PROPERTIES. Acta Pathol Microbiol Scand. 1963;58:493–500. doi: 10.1111/j.1699-0463.1963.tb01596.x. [DOI] [PubMed] [Google Scholar]
  10. Nagel J. G., Tuazon C. U., Cardella T. A., Sheagren J. N. Teichoic acid serologic diagnosis of staphylococcal endocarditis. Use of gel diffusion and counterimmunoelectrophoretic methods. Ann Intern Med. 1975 Jan;82(1):13–17. doi: 10.7326/0003-4819-82-1-13. [DOI] [PubMed] [Google Scholar]
  11. OEDING P. Agglutinability of pyogenic staphylococci at various conditions. Acta Pathol Microbiol Scand. 1957;41(4):310–324. doi: 10.1111/j.1699-0463.1957.tb01028.x. [DOI] [PubMed] [Google Scholar]
  12. Oeding P., Digranes A. Classification of coagulase-negative staphylococci in the diagnostic laboratory. Acta Pathol Microbiol Scand B. 1977 Apr;85(2):136–142. doi: 10.1111/j.1699-0463.1977.tb01687.x. [DOI] [PubMed] [Google Scholar]
  13. Perlman B. B., Freedman L. R. Experimental endocarditis. II. Staphylococcal infection of the aortic valve following placement of a polyethylene catheter in the left side of the heart. Yale J Biol Med. 1971 Oct;44(2):206–213. [PMC free article] [PubMed] [Google Scholar]
  14. Peterson P. K., Wilkinson B. J., Kim Y., Schmeling D., Douglas S. D., Quie P. G., Verhoef J. The key role of peptidoglycan in the opsonization of Staphylococcus aureus. J Clin Invest. 1978 Mar;61(3):597–609. doi: 10.1172/JCI108971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. SMITH I. M., BEALS P. D., KINGSBURY K. R., HASENCLEVER H. F. Observations on Staphylococcus albus septicemia in mice and men. AMA Arch Intern Med. 1958 Sep;102(3):375–388. doi: 10.1001/archinte.1958.00030010375005. [DOI] [PubMed] [Google Scholar]
  16. Schoenbaum S. C., Gardner P., Shillito J. Infections of cerebrospinal fluid shunts: epidemiology, clinical manifestations, and therapy. J Infect Dis. 1975 May;131(5):543–552. doi: 10.1093/infdis/131.5.543. [DOI] [PubMed] [Google Scholar]
  17. Tuazon C. U., Sheagren J. N., Choa M. S., Marcus D., Curtin J. A. Staphylococcus aureus bacteremia: relationship between formation of antibodies to teichoic acid and development of metastatic abscesses. J Infect Dis. 1978 Jan;137(1):57–62. doi: 10.1093/infdis/137.1.57. [DOI] [PubMed] [Google Scholar]
  18. Verbrugh H. A., Peters R., Rozenberg-Arska M., Peterson P. K., Verhoef J. Antibodies to cell wall peptidoglycan of Staphylococcus aureus in patients with serious staphylococcal infections. J Infect Dis. 1981 Jul;144(1):1–9. doi: 10.1093/infdis/144.1.1. [DOI] [PubMed] [Google Scholar]
  19. Wheat L. J., Kohler R. B., White A. Solid-phase radioimmunoassay for detection of staphylococcal antigen in serum of rabbits with endocraditis due to Staphylococcus aureus. J Infect Dis. 1978 Aug;138(2):174–180. doi: 10.1093/infdis/138.2.174. [DOI] [PubMed] [Google Scholar]
  20. Wicken A. J., Knox K. W. Lipoteichoic acids: a new class of bacterial antigen. Science. 1975 Mar 28;187(4182):1161–1167. doi: 10.1126/science.46620. [DOI] [PubMed] [Google Scholar]

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