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. 1983 Dec;42(3):1086–1091. doi: 10.1128/iai.42.3.1086-1091.1983

Enterobacterial common antigen-tetanus toxoid conjugate as immunogen.

C Lugowski, M Kułakowska, E Romanowska
PMCID: PMC264411  PMID: 6358039

Abstract

The methods of limited periodate oxidation and reductive amination were used to obtain covalently linked enterobacterial common antigen (ECA) with tetanus toxoid. This procedure is simple and gives a good yield of the conjugate with high ECA content (molecular ratio of ECA to tetanus toxoid, 6:1). The ECA-tetanus toxoid conjugate is immunogenic in rabbits, in contrast to free ECA or a mixture of ECA with proteins. This conjugate produces high levels of ECA-specific immunoglobulin G antibodies, which can be used as a standard serum.

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Selected References

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