Skip to main content
NCBI home page
Infection and Immunity logoLink to Infection and Immunity
. 1983 Nov;42(2):778–782. doi: 10.1128/iai.42.2.778-782.1983

Effects of lincomycin and tetracycline on production and properties of enterotoxins of enterotoxigenic Escherichia coli.

M Yoh, K Yamamoto, T Honda, Y Takeda, T Miwatani
PMCID: PMC264497  PMID: 6358032

Abstract

Enterotoxigenic Escherichia coli grown in the presence of lincomycin and tetracycline produced an increased amount of heat-labile enterotoxin (LT). These antibiotics increased the production of not only extracellular LT but also intracellular LT. On the other hand, lincomycin did not stimulate the production of heat-stable enterotoxin by enterotoxigenic E. coli. The extracellular LTs produced in the presence of lincomycin and tetracycline were purified and analyzed by sodium dodecyl sulfate-polyacrylamide gel disc electrophoresis. Results showed that the A subunits of the purified LTs were not nicked, unlike that of extracellular LT produced in the absence of the antibiotics.

Full text

PDF
778

Images in this article

780Image
on p.780
781Image
on p.781

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Clements J. D., Finkelstein R. A. Isolation and characterization of homogeneous heat-labile enterotoxins with high specific activity from Escherichia coli cultures. Infect Immun. 1979 Jun;24(3):760–769. doi: 10.1128/iai.24.3.760-769.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Evans D. J., Jr, Evans D. G., Gorbach S. L. Production of vascular permeability factor by enterotoxigenic Escherichia coli isolated from man. Infect Immun. 1973 Nov;8(5):725–730. doi: 10.1128/iai.8.5.725-730.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Fairbanks G., Steck T. L., Wallach D. F. Electrophoretic analysis of the major polypeptides of the human erythrocyte membrane. Biochemistry. 1971 Jun 22;10(13):2606–2617. doi: 10.1021/bi00789a030. [DOI] [PubMed] [Google Scholar]
  4. Finkelstein R. A., LoSpalluto J. J. Pathogenesis of experimental cholera. Preparation and isolation of choleragen and choleragenoid. J Exp Med. 1969 Jul 1;130(1):185–202. doi: 10.1084/jem.130.1.185. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Guerrant R. L., Brunton L. L., Schnaitman T. C., Rebhun L. I., Gilman A. G. Cyclic adenosine monophosphate and alteration of Chinese hamster ovary cell morphology: a rapid, sensitive in vitro assay for the enterotoxins of Vibrio cholerae and Escherichia coli. Infect Immun. 1974 Aug;10(2):320–327. doi: 10.1128/iai.10.2.320-327.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Honda T., Hernadez I., Katoh T., Miwatani T. Stimulation of enterotoxin production of Clostridium difficile ny antibiotics. Lancet. 1983 Mar 19;1(8325):655–655. doi: 10.1016/s0140-6736(83)91832-9. [DOI] [PubMed] [Google Scholar]
  7. Honda T., Shimizu M., Takeda Y., Miwatani T. Isolation of a factor causing morphological changes of chinese hamster ovary cells from the culture filtrate of Vibrio parahaemolyticus. Infect Immun. 1976 Oct;14(4):1028–1033. doi: 10.1128/iai.14.4.1028-1033.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Honda T., Tsuji T., Takeda Y., Miwatani T. Immunological nonidentity of heat-labile enterotoxins from human and porcine enterotoxigenic Escherichia coli. Infect Immun. 1981 Nov;34(2):337–340. doi: 10.1128/iai.34.2.337-340.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Kunkel S. L., Robertson D. C. Purification and chemical characterization of the heat-labile enterotoxin produced by enterotoxigenic Escherichia coli. Infect Immun. 1979 Aug;25(2):586–596. doi: 10.1128/iai.25.2.586-596.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Levner M. H., Urbano C., Rubin B. A. Lincomycin increases synthetic rate and periplasmic pool size for cholera toxin. J Bacteriol. 1980 Jul;143(1):441–447. doi: 10.1128/jb.143.1.441-447.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Levner M., Wiener F. P., Rubin B. A. Induction of Escherichia coli and Vibrio cholerae enterotoxins by an inhibitor of protein synthesis. Infect Immun. 1977 Jan;15(1):132–137. doi: 10.1128/iai.15.1.132-137.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. So M., Crosa J. H., Falkow S. Polynucleotide sequence relationships among Ent plasmids and the relationship between Ent and other plasmids. J Bacteriol. 1975 Jan;121(1):234–238. doi: 10.1128/jb.121.1.234-238.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Takeda Y., Honda T., Taga S., Miwatani T. In vitro formation of hybrid toxins between subunits of Escherichia coli heat-labile enterotoxin and those of cholera enterotoxin. Infect Immun. 1981 Nov;34(2):341–346. doi: 10.1128/iai.34.2.341-346.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Takeda Y., Takeda T., Yano T., Yamamoto K., Miwatani T. Purification and partial characterization of heat-stable enterotoxin of enterotoxigenic Escherichia coli. Infect Immun. 1979 Sep;25(3):978–985. doi: 10.1128/iai.25.3.978-985.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Tsuji T., Taga S., Honda T., Takeda Y., Miwatani T. Molecular heterogeneity of heat-labile enterotoxins from human and porcine enterotoxigenic Escherichia coli. Infect Immun. 1982 Nov;38(2):444–448. doi: 10.1128/iai.38.2.444-448.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Yamamoto K., Takeda Y., Miwatani T., Craig J. P. Purification and some properties of a non-o1 Vibrio cholerae enterotoxin that is identical to cholera enterotoxin. Infect Immun. 1983 Mar;39(3):1128–1135. doi: 10.1128/iai.39.3.1128-1135.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Infection and Immunity are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES