Skip to main content
NCBI home page
Infection and Immunity logoLink to Infection and Immunity
. 1983 Oct;42(1):427–430. doi: 10.1128/iai.42.1.427-430.1983

Evidence indicating that the cholera toxin structural genes of Vibrio cholerae RJ1 and 3083-2 are between met and trp.

D W Saunders, G J Kubala, A B Vaidya, M G Bramucci
PMCID: PMC264577  PMID: 6311752

Abstract

An enterotoxin A subunit-specific radioactive probe was used to correlate expression of vct-1 or vct-2 with the presence of specific restriction fragments in the DNAs of several wild-type and recombinant Vibrio cholerae strains. The data are consistent with the conclusion that vct is a cholera toxin structural gene.

Full text

PDF
427

Images in this article

428Image
on p.428

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Dallas W. S., Gill D. M., Falkow S. Cistrons encoding Escherichia coli heat-labile toxin. J Bacteriol. 1979 Sep;139(3):850–858. doi: 10.1128/jb.139.3.850-858.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Datta A., Parker C. D., Wohlhieter J. A., Baron L. S. Isolation and characterization of the fertility factor P of Vibrio cholerae. J Bacteriol. 1973 Feb;113(2):763–771. doi: 10.1128/jb.113.2.763-771.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Greene P. J., Heyneker H. L., Bolivar F., Rodriguez R. L., Betlach M. C., Covarrubias A. A., Backman K., Russel D. J., Tait R., Boyer H. W. A general method for the purification of restriction enzymes. Nucleic Acids Res. 1978 Jul;5(7):2373–2380. doi: 10.1093/nar/5.7.2373. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Hanne L. F., Howe T. R., Iglewski B. H. Locus of the Pseudomonas aeruginosa toxin A gene. J Bacteriol. 1983 Apr;154(1):383–386. doi: 10.1128/jb.154.1.383-386.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Holmes R. K., Baine W. B., Vasil M. L. Quantitative measurements of cholera enterotoxin in cultures of toxinogenic wild-type and nontoxinogenic mutant strains of Vibrio cholerae by using a sensitive and specific reversed passive hemagglutination assay for cholera enerotoxin. Infect Immun. 1978 Jan;19(1):101–106. doi: 10.1128/iai.19.1.101-106.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Holmgren J., Lange S., Lönnroth I. Reversal of cyclic AMP-mediated intestinal secretion in mice by chlorpromazine. Gastroenterology. 1978 Dec;75(6):1103–1108. [PubMed] [Google Scholar]
  7. Kaper J. B., Moseley S. L., Falkow S. Molecular characterization of environmental and nontoxigenic strains of Vibrio cholerae. Infect Immun. 1981 May;32(2):661–667. doi: 10.1128/iai.32.2.661-667.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Long C. A., Dumaswala U. J., Tancin S. L., Vaidya A. B. Organization and expression of endogenous murine mammary tumor virus genes in mice congenic at the H-2 complex. Virology. 1980 May;103(1):167–177. doi: 10.1016/0042-6822(80)90135-x. [DOI] [PubMed] [Google Scholar]
  9. Pearson G. D., Mekalanos J. J. Molecular cloning of Vibrio cholerae enterotoxin genes in Escherichia coli K-12. Proc Natl Acad Sci U S A. 1982 May;79(9):2976–2980. doi: 10.1073/pnas.79.9.2976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Saunders D. W., Bramucci M. G. Genetic mapping of the tox-1000 locus of Vibrio cholerae El Tor strain RJ1. Infect Immun. 1983 May;40(2):829–831. doi: 10.1128/iai.40.2.829-831.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Saunders D. W., Schanbacher K. J., Bramucci M. G. Mapping of a gene in Vibrio cholerae that determines the antigenic structure of cholera toxin. Infect Immun. 1982 Dec;38(3):1109–1116. doi: 10.1128/iai.38.3.1109-1116.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Southern E. M. Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol. 1975 Nov 5;98(3):503–517. doi: 10.1016/s0022-2836(75)80083-0. [DOI] [PubMed] [Google Scholar]
  13. Vasil M. L., Holmes R. K., Finkelstein R. A. Conjugal transfer of a chromosomal gene determining production of enterotoxin in vibrio cholerae. Science. 1975 Mar 7;187(4179):849–850. doi: 10.1126/science.1114331. [DOI] [PubMed] [Google Scholar]

Articles from Infection and Immunity are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES