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. Author manuscript; available in PMC: 2009 Feb 20.
Published in final edited form as: Biochemistry. 2008 Nov 25;47(47):12409–12419. doi: 10.1021/bi8015444

Figure 1.

Figure 1

Electrophoretic characterization of purified native and reconstituted Gt. (a) Separation of native Gt and reconstituted Gt after gel filtration on 12% SDS-PAGE gels (2 μg) and on a 16.5% Tris/Tricine polyacrylamide gel (5 μg). The Gt α, β, and γ subunits present in these protein preparations were confirmed by immunoblotting (WB) analysis. (b) Native electrophoresis of 2 μg of Gt on 7.5% polyarylamide gels and confirmation of the subunits present in separated bands by immunoblotting (WB). Key: §, front of dye from sample loading buffer; *, Gtα; **, Gt βγ; ***, Gtα.