Skip to main content
NCBI home page
Infection and Immunity logoLink to Infection and Immunity
. 1983 Sep;41(3):1138–1143. doi: 10.1128/iai.41.3.1138-1143.1983

Effect of heptakis (2,6-O-dimethyl) beta-cyclodextrin on the production of pertussis toxin by Bordetella pertussis.

A Imaizumi, Y Suzuki, S Ono, H Sato, Y Sato
PMCID: PMC264618  PMID: 6309661

Abstract

The effect of heptakis (2,6-O-dimethyl) beta-cyclodextrin (Me beta CD) on the production of pertussis toxin was evaluated. The addition of Me beta CD to the medium stimulated cell growth and pertussis toxin production. Me beta CD enhanced pertussis toxin production 100 times more in synthetic media, such as Stainer-Scholte medium (D. W. Stainer and M. J. Scholte, J. Gen. Microbiol. 63:211-220), than in Me beta CD-free medium in 2-day shake cultures. Maximum production of pertussis toxin was estimated as 50 mg of protein per liter of culture broth both by in vitro and in vivo assays. Purified toxin was demonstrated to be biochemically and biologically identical to the toxin produced in Me beta CD-free static cultures.

Full text

PDF
1138

Images in this article

1142Image
on p.1142

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Imaizumi A., Suzuki Y., Ono S., Sato H., Sato Y. Heptakis(2,6-O-dimethyl)beta-cyclodextrin: a novel growth stimulant for Bordetella pertussis phase I. J Clin Microbiol. 1983 May;17(5):781–786. doi: 10.1128/jcm.17.5.781-786.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Irons L. I., MacLennan A. P. Isolation of the lymphocytosis promoting factor-haemagglutinin of Bordetella pertussis by affinity chromatography. Biochim Biophys Acta. 1979 Sep 29;580(1):175–185. doi: 10.1016/0005-2795(79)90208-3. [DOI] [PubMed] [Google Scholar]
  3. Katada T., Ui M. ADP ribosylation of the specific membrane protein of C6 cells by islet-activating protein associated with modification of adenylate cyclase activity. J Biol Chem. 1982 Jun 25;257(12):7210–7216. [PubMed] [Google Scholar]
  4. Lehrer S. B., Tan E. M., Vaughan J. H. Extraction and partial purification of the histamine-sensitizing factor of Bordetella pertussis. J Immunol. 1974 Jul;113(1):18–26. [PubMed] [Google Scholar]
  5. Morse S. I., Morse J. H. Isolation and properties of the leukocytosis- and lymphocytosis-promoting factor of Bordetella pertussis. J Exp Med. 1976 Jun 1;143(6):1483–1502. doi: 10.1084/jem.143.6.1483. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Munoz J. J., Arai H., Cole R. L. Mouse-protecting and histamine-sensitizing activities of pertussigen and fimbrial hemagglutinin from Bordetella pertussis. Infect Immun. 1981 Apr;32(1):243–250. doi: 10.1128/iai.32.1.243-250.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Parker C. Role of the genetics and physiology of Bordetella pertussis in the production of vaccine and the study of host-parasite relationships in pertussis. Adv Appl Microbiol. 1976;20:27–42. doi: 10.1016/s0065-2164(08)70107-2. [DOI] [PubMed] [Google Scholar]
  8. Pittman M. Pertussis toxin: the cause of the harmful effects and prolonged immunity of whooping cough. A hypothesis. Rev Infect Dis. 1979 May-Jun;1(3):401–412. doi: 10.1093/clinids/1.3.401. [DOI] [PubMed] [Google Scholar]
  9. REISFELD R. A., LEWIS U. J., WILLIAMS D. E. Disk electrophoresis of basic proteins and peptides on polyacrylamide gels. Nature. 1962 Jul 21;195:281–283. doi: 10.1038/195281a0. [DOI] [PubMed] [Google Scholar]
  10. ROWATT E. The growth of Bordetella pertussis: a review. J Gen Microbiol. 1957 Oct;17(2):297–326. doi: 10.1099/00221287-17-2-297. [DOI] [PubMed] [Google Scholar]
  11. Stainer D. W., Scholte M. J. A simple chemically defined medium for the production of phase I Bordetella pertussis. J Gen Microbiol. 1970 Oct;63(2):211–220. doi: 10.1099/00221287-63-2-211. [DOI] [PubMed] [Google Scholar]
  12. Tilden E. B., Hudson C. S. Preparation and Properties of the Amylases Produced by Bacillus macerans and Bacillus polymyxa. J Bacteriol. 1942 Apr;43(4):527–544. doi: 10.1128/jb.43.4.527-544.1942. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Yajima M., Hosoda K., Kanbayashi Y., Nakamura T., Takahashi I., Ui M. Biological properties of islets-activating protein (IAP) purified from the culture medium of Bordetella pertussis. J Biochem. 1978 Jan;83(1):305–312. doi: 10.1093/oxfordjournals.jbchem.a131905. [DOI] [PubMed] [Google Scholar]

Articles from Infection and Immunity are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES