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. 1983 Sep;41(3):1144–1149. doi: 10.1128/iai.41.3.1144-1149.1983

Natural macrophage cytotoxicity against Trichomonas vaginalis is mediated by soluble lytic factors.

M G Martinotti, F Cofano, P Martinetto, S Landolfo
PMCID: PMC264619  PMID: 6604025

Abstract

Mechanisms of lysis of the extracellular protozoan Trichomonas vaginalis by uninduced resident macrophages were analyzed. Supernatants obtained by culturing such macrophages with T. vaginalis were cytotoxic for the protozoa in a dose-dependent manner. Supernatants from macrophages cultured alone were cytotoxic at lower levels, whereas those obtained from T. vaginalis alone and from macrophages cultured with unrelated cells (B77) were not cytotoxic. Cytotoxic activity appeared after 4 h of contact between effectors and target cells and reached a plateau at 18 to 24 h. Microtubule disrupting agents (colchicine and vinblastine) enhanced protozoan lysis, whereas cytochalasin B, an inhibitor of microfilaments, completely blocked T. vaginalis lysis. Treatment of macrophages with protein synthesis inhibitors (cycloheximide and puromycin) impaired effector cytotoxicity. Lytic activity remained after dialysis of supernatants, treatment with 10% bovine fetal serum, and treatment at 56 degrees C for 1 h, but it was completely prevented by treatment at 90 degrees C for 10 min. In conclusion, our data show that natural cytotoxicity against T. vaginalis is performed by normal resident macrophages through the release of at least two heterogeneous soluble factors.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Adams D. O., Johnson W. J., Marino P. A. Mechanisms of target recognition and destruction in macrophage-mediated tumor cytotoxicity. Fed Proc. 1982 Apr;41(6):2212–2221. [PubMed] [Google Scholar]
  2. Adams D. O., Kao K. J., Farb R., Pizzo S. V. Effector mechanisms of cytolytically activated macrophages. II. Secretion of a cytolytic factor by activated macrophages and its relationship to secreted neutral proteases. J Immunol. 1980 Jan;124(1):293–300. [PubMed] [Google Scholar]
  3. Allison A. C., Ferluga J. Editorial: How lymphocytes kill tumor cells. N Engl J Med. 1976 Jul 15;295(3):165–167. doi: 10.1056/NEJM197607152950311. [DOI] [PubMed] [Google Scholar]
  4. Carpén O., Virtanen I., Saksela E. The cytotoxic activity of human natural killer cells requires an intact secretory apparatus. Cell Immunol. 1981 Feb;58(1):97–106. doi: 10.1016/0008-8749(81)90152-0. [DOI] [PubMed] [Google Scholar]
  5. Currie G. A. Activated macrophages kill tumour cells by releasing arginase. Nature. 1978 Jun 29;273(5665):758–759. doi: 10.1038/273758a0. [DOI] [PubMed] [Google Scholar]
  6. GOODMAN G. T., KOPROWSKI H. Macrophages as a cellular expression of inherited natural resistance. Proc Natl Acad Sci U S A. 1962 Feb;48:160–165. doi: 10.1073/pnas.48.2.160. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Gordon S., Werb Z. Secretion of macrophage neutral proteinase is enhanced by colchicine. Proc Natl Acad Sci U S A. 1976 Mar;73(3):872–876. doi: 10.1073/pnas.73.3.872. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Landolfo S., Martinotti M. G., Martinetto P., Forni G. Natural cell-mediated cytotoxicity against Trichomonas vaginalis in the mouse. I. Tissue, strain, age distribution, and some characteristics of the effector cells. J Immunol. 1980 Feb;124(2):508–514. [PubMed] [Google Scholar]
  9. Mantovani A., Polentarutti N., Peri G., Martinotti G., Landolfo S. Cytotoxicity of human peripheral blood monocytes against Trichomonas vaginalis. Clin Exp Immunol. 1981 Nov;46(2):391–396. [PMC free article] [PubMed] [Google Scholar]
  10. Marzella L., Glaumann H. Increased degradation in rat liver induced by vinblastine. II. Morphologic characterization. Lab Invest. 1980 Jan;42(1):18–27. [PubMed] [Google Scholar]
  11. McLeod R., Bensch K. G., Smith S. M., Remington J. S. Effects of human peripheral blood monocytes, monocyte-derived macrophages, and spleen mononuclear phagocytes on Toxoplasma gondii. Cell Immunol. 1980 Sep 1;54(2):330–350. doi: 10.1016/0008-8749(80)90214-2. [DOI] [PubMed] [Google Scholar]
  12. Nathan C. F., Brukner L. H., Silverstein S. C., Cohn Z. A. Extracellular cytolysis by activated macrophages and granulocytes. I. Pharmacologic triggering of effector cells and the release of hydrogen peroxide. J Exp Med. 1979 Jan 1;149(1):84–99. doi: 10.1084/jem.149.1.84. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Nathan C. F., Murray H. W., Cohn Z. A. The macrophage as an effector cell. N Engl J Med. 1980 Sep 11;303(11):622–626. doi: 10.1056/NEJM198009113031106. [DOI] [PubMed] [Google Scholar]
  14. Smith P. D., Elson C. O., Keister D. B., Nash T. E. Human host response to Giardia lamblia. I. Spontaneous killing by mononuclear leukocytes in vitro. J Immunol. 1982 Mar;128(3):1372–1376. [PubMed] [Google Scholar]
  15. Unanue E. R. The regulation of lymphocyte functions by the macrophage. Immunol Rev. 1978;40:227–255. doi: 10.1111/j.1600-065x.1978.tb00408.x. [DOI] [PubMed] [Google Scholar]

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