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. 1983 Sep;41(3):1363–1367. doi: 10.1128/iai.41.3.1363-1367.1983

Analysis of macrophage interactions with cryopreserved amastigotes of Leishmania tropica.

D L Hoover, C A Nacy
PMCID: PMC264647  PMID: 6885164

Abstract

Amastigotes of Leishmania tropica were harvested from infected footpads of BALB/cJ mice and cryopreserved, using a controlled-rate freezer. This technique produced inocula with reproducible viability and infectivity for peritoneal macrophages from C3H/HeN mice. Cryopreserved amastigotes, stored for up to 17 weeks in a liquid nitrogen freezer, were similar to freshly harvested parasites in susceptibility to lymphokine-induced intracellular killing. Interactions of cryopreserved parasites with macrophages were best evaluated at 72 h, since macrophages preferentially ingested viable amastigotes and rapidly cleared ingested nonviable parasites.

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Selected References

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