Skip to main content
PMC home page
Infection and Immunity logoLink to Infection and Immunity
. 1983 Aug;41(2):516–522. doi: 10.1128/iai.41.2.516-522.1983

Modulation of Bordetella pertussis by nicotinic acid.

W L McPheat, A C Wardlaw, P Novotny
PMCID: PMC264671  PMID: 6307872

Abstract

Growth of Bordetella pertussis in a high concentration of nicotinic acid (NA) had a modulating effect on several properties and activities of the bacteria. Compared with normally grown cells, those grown in a high concentration of NA had reduced capacity for taking up both NA and nicotinamide (ND); they had reduced adenylate cyclase activity and showed loss of agglutinogen factors 2 and 3, but an increase in factor 1. By contrast, cells grown in a high concentration of ND showed only a slightly decreased capacity for uptake of ND and none of the other changes. Modulation of B. pertussis by NA varied with the strain and culture conditions and appeared to be distinct from the antigenic modulation induced by high Mg2+ in the culture medium. Evidence is presented for the association of a small proportion of the extracytoplasmic adenylate cyclase with the outer membrane of B. pertussis.

Full text

PDF

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ANDERSEN E. K. Serological studies on H. pertussis H. parapertussis and H. bronchisepticus. Acta Pathol Microbiol Scand. 1953;33(2):202–224. doi: 10.1111/j.1699-0463.1953.tb01512.x. [DOI] [PubMed] [Google Scholar]
  2. Ackers J. P., Dolby J. M. The antigen of Bordetella pertussis that induces bactericidal antibody and its relationship to protection of mice. J Gen Microbiol. 1972 Apr;70(2):371–382. doi: 10.1099/00221287-70-2-371. [DOI] [PubMed] [Google Scholar]
  3. Adams G. J. Intracerebral infection of mice with high-virulence and low-virulence strains of Bordetella pertussis. J Med Microbiol. 1970 Feb;3(1):1–13. doi: 10.1099/00222615-3-1-1. [DOI] [PubMed] [Google Scholar]
  4. Baecker P. A., Yung S. G., Rodriguez M., Austin E., Andreoli A. J. Periplasmic localization of nicotinate phosphoribosyltransferase in Escherichia coli. J Bacteriol. 1978 Mar;133(3):1108–1112. doi: 10.1128/jb.133.3.1108-1112.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Berkowitz S. A., Goldhammer A. R., Hewlett E. L., Wolff J. Activation of prokaryotic adenylate cyclase by calmodulin. Ann N Y Acad Sci. 1980;356:360–360. doi: 10.1111/j.1749-6632.1980.tb29626.x. [DOI] [PubMed] [Google Scholar]
  6. Cohen S. M., Wheeler M. W. Pertussis Vaccine Prepared with Phase-I Cultures Grown in Fluid Medium. Am J Public Health Nations Health. 1946 Apr;36(4):371–376. [PMC free article] [PubMed] [Google Scholar]
  7. Confer D. L., Eaton J. W. Phagocyte impotence caused by an invasive bacterial adenylate cyclase. Science. 1982 Sep 3;217(4563):948–950. doi: 10.1126/science.6287574. [DOI] [PubMed] [Google Scholar]
  8. Cowell J. L., Hewlett E. L., Manclark C. R. Intracellular localization of the dermonecrotic toxin of Bordetella pertussis. Infect Immun. 1979 Sep;25(3):896–901. doi: 10.1128/iai.25.3.896-901.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Foster J. W., Kinney D. M., Moat A. G. Pyridine nucleotide cycle of Salmonella typhimurium: regulation of nicotinic acid phosphoribosyltransferase and nicotinamide deamidase. J Bacteriol. 1979 Jun;138(3):957–961. doi: 10.1128/jb.138.3.957-961.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Foster J. W., Moat A. G. Nicotinamide adenine dinucleotide biosynthesis and pyridine nucleotide cycle metabolism in microbial systems. Microbiol Rev. 1980 Mar;44(1):83–105. doi: 10.1128/mr.44.1.83-105.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Gilman A. G. A protein binding assay for adenosine 3':5'-cyclic monophosphate. Proc Natl Acad Sci U S A. 1970 Sep;67(1):305–312. doi: 10.1073/pnas.67.1.305. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Hewlett E. L., Urban M. A., Manclark C. R., Wolff J. Extracytoplasmic adenylate cyclase of Bordetella pertussis. Proc Natl Acad Sci U S A. 1976 Jun;73(6):1926–1930. doi: 10.1073/pnas.73.6.1926. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Hewlett E. L., Wolff J., Manclark C. R. Regulation of Bordetella pertussis extracytoplasmic adenylate cyclase. Adv Cyclic Nucleotide Res. 1978;9:621–628. [PubMed] [Google Scholar]
  14. Hewlett E., Wolff J. Soluble adenylate cyclase from the culture medium of Bordetella pertussis: purification and characterization. J Bacteriol. 1976 Aug;127(2):890–898. doi: 10.1128/jb.127.2.890-898.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Idigbe E. O., Parton R., Wardlaw A. C. Rapidity of antigenic modulation of Bordetella pertussis in modified Hornibrook medium. J Med Microbiol. 1981 Nov;14(4):409–418. doi: 10.1099/00222615-14-4-409. [DOI] [PubMed] [Google Scholar]
  16. JEBB W. H., TOMLINSON A. H. The nutritional requirements of Haemophilus pertussis. J Gen Microbiol. 1955 Aug;13(1):1–8. doi: 10.1099/00221287-13-1-1. [DOI] [PubMed] [Google Scholar]
  17. LACEY B. W. Antigenic modulation of Bordetella pertussis. J Hyg (Lond) 1960 Mar;58:57–93. doi: 10.1017/s0022172400038134. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. LACEY B. W. Antigenic modulation of Haemophilus pertussis. J Gen Microbiol. 1951 Aug;5(3):xxi–xxi. [PubMed] [Google Scholar]
  19. McPheat W. L., Wardlaw A. C. Inhibition of nicotinic acid and nicotinamide uptake into Bordetella pertussis by structural analogues. J Gen Microbiol. 1982 Nov;128(11):2681–2685. doi: 10.1099/00221287-128-11-2681. [DOI] [PubMed] [Google Scholar]
  20. Munoz J. J., Arai H., Bergman R. K., Sadowski P. L. Biological activities of crystalline pertussigen from Bordetella pertussis. Infect Immun. 1981 Sep;33(3):820–826. doi: 10.1128/iai.33.3.820-826.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Neujahr H. Y., Varga Z. Transport of B-vitamins in microorganisms. VII. The uptake of 14 C-niacinamide by non-proliferating cells and by protoplasts of Streptococcus faecalis. Acta Chem Scand. 1966;20(6):1529–1534. doi: 10.3891/acta.chem.scand.20-1529. [DOI] [PubMed] [Google Scholar]
  22. ROWATT E. Some factors affecting the growth of Bordetella pertussis. J Gen Microbiol. 1957 Oct;17(2):279–296. doi: 10.1099/00221287-17-2-279. [DOI] [PubMed] [Google Scholar]
  23. Schneider D. R., Parker C. D. Effect of pyridines on phenotypic properties of Bordetella pertussis. Infect Immun. 1982 Nov;38(2):548–553. doi: 10.1128/iai.38.2.548-553.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Stainer D. W., Scholte M. J. A simple chemically defined medium for the production of phase I Bordetella pertussis. J Gen Microbiol. 1970 Oct;63(2):211–220. doi: 10.1099/00221287-63-2-211. [DOI] [PubMed] [Google Scholar]
  25. Wardlaw A. C., Parton R., Bergman R. K., Munoz J. J. Loss of adjuvanticity in rats for the hyperacute form of allergic encephalomyelitis and for reaginic antibody production in mice of a phenotypic variant of Bordetella pertussis. Immunology. 1979 Jul;37(3):539–545. [PMC free article] [PubMed] [Google Scholar]
  26. Wardlaw A. C., Parton R., Hooker M. J. Loss of protective antigen, histamine-sensitising factor and envelope polypeptides in cultural variants of Bordetella pertussis. J Med Microbiol. 1976 Feb;9(1):89–100. doi: 10.1099/00222615-9-1-89. [DOI] [PubMed] [Google Scholar]
  27. Wolff J., Cook G. H., Goldhammer A. R., Berkowitz S. A. Calmodulin activates prokaryotic adenylate cyclase. Proc Natl Acad Sci U S A. 1980 Jul;77(7):3841–3844. doi: 10.1073/pnas.77.7.3841. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Infection and Immunity are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES