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. 1983 Aug;41(2):540–545. doi: 10.1128/iai.41.2.540-545.1983

Hemagglutinin-specific antibody responses in immunoglobulin G, A, and M isotypes as measured by enzyme-linked immunosorbent assay after primary or secondary infection of humans with influenza A virus.

D B Burlington, M L Clements, G Meiklejohn, M Phelan, B R Murphy
PMCID: PMC264675  PMID: 6874068

Abstract

The isotype-specific antibody responses to purified hemagglutinin of adults undergoing either primary or secondary infection with an influenza A virus were characterized by using an enzyme-linked immunosorbent assay. Twenty-eight military recruits undergoing primary infection with A/USSR/92/77 (H1N1)-like virus had serum antibody rises in the immunoglobulin M (IgM) (86%), IgG (100%), and IgA (96%) isotypes. In contrast, 19 adult volunteers undergoing secondary infection with A/Peking/2/79 (H3N2) wild-type virus had serum antibody titer rises largely restricted to the IgG (68%) and IgA (74%) classes, with only 1 volunteer having a serum IgM antibody titer rise. Nasal wash hemagglutinin-specific antibody responses in the adults undergoing secondary infection were predominantly in the IgA class (74%). There was a correlation between the presence of and the magnitude of nasal wash and serum hemagglutinin-specific IgA antibody responses in these adults. This suggested that there was a common source for the hemagglutinin-specific local IgA antibody and serum IgA antibody produced after infection. The recruits undergoing primary H1N1 influenza virus infection had H1 hemagglutinin-specific enzyme-linked immunosorbent assay antibody in each of the IgA, IgG, and IgM isotypes in their acute-phase serum. However, no role for this cross-reactive antibody in modifying the severity of illness experienced by the recruits could be demonstrated.

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Selected References

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  1. Buchner Y. I., Heath R. B., Collins J. V., Pattison J. R. Serum IgM antibody and influenza A infection. J Clin Pathol. 1977 Aug;30(8):723–727. doi: 10.1136/jcp.30.8.723. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Chanock R. M., Murphy B. R. Use of temperature-sensitive and cold-adapted mutant viruses in immunoprophylaxis of acute respiratory tract disease. Rev Infect Dis. 1980 May-Jun;2(3):421–432. doi: 10.1093/clinids/2.3.421. [DOI] [PubMed] [Google Scholar]
  3. Cukor G., Nowak N. A., Blacklow N. R. Immunoglobulin M responses to the Norwalk virus of gastroenteritis. Infect Immun. 1982 Aug;37(2):463–468. doi: 10.1128/iai.37.2.463-468.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Daugharty H., Davis M. L., Kaye H. S. Immunoglobulin class of influenza antibodies investigated by radioimmunoassay (RIA). J Immunol. 1972 Oct;109(4):849–856. [PubMed] [Google Scholar]
  5. Dörries R., ter Meulen V. Detection of enterovirus specific IgG and IgM antibodies in humans by an indirect solid phase radioimmunoassay. Med Microbiol Immunol. 1980;168(3):159–171. doi: 10.1007/BF02122850. [DOI] [PubMed] [Google Scholar]
  6. FAZEKAS de ST GROTH S. Influenza: a study in mice. Lancet. 1950 Jun 17;1(6616):1101–1105. doi: 10.1016/s0140-6736(50)91904-0. [DOI] [PubMed] [Google Scholar]
  7. Francis T., Jr A RATIONALE FOR STUDIES IN THE CONTROL OF EPIDEMIC INFLUENZA. Science. 1943 Mar 12;97(2515):229–235. doi: 10.1126/science.97.2515.229. [DOI] [PubMed] [Google Scholar]
  8. Gonchoroff N. J., Kendal A. P., Phillips D. J., Reimer C. B. Immunoglobulin M and G antibody response to type- and subtype-specific antigens after primary and secondary exposures of mice to influenza A viruses. Infect Immun. 1982 May;36(2):510–517. doi: 10.1128/iai.36.2.510-517.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Kalimo K. O., Marttila R. J., Granfors K., Viljanen M. K. Solid-phase radioimmunoassay of human immunoglobulin M and immunoglobulin G antibodies against herpes simplex virus type 1 capsid, envelope, and excreted antigens. Infect Immun. 1977 Mar;15(3):883–889. doi: 10.1128/iai.15.3.883-889.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Lerner A. M., Shippey M. J., Crane L. R. Serologic responses to herpes simplex virus in rabbits: complement-requiring neutralizing, conventional neutralizing, and passive hemagglutinating antibodies. J Infect Dis. 1974 Jun;129(6):623–636. doi: 10.1093/infdis/129.6.623. [DOI] [PubMed] [Google Scholar]
  11. Linnemann C. C., Hegg M. E., Rotte T. C., Phair J. P., Schiff G. M. Measles IgM response during reinfection of previously vaccinated children. J Pediatr. 1973 May;82(5):798–801. doi: 10.1016/s0022-3476(73)80069-1. [DOI] [PubMed] [Google Scholar]
  12. Murphy B. R., Nelson D. L., Wright P. F., Tierney E. L., Phelan M. A., Chanock R. M. Secretory and systemic immunological response in children infected with live attenuated influenza A virus vaccines. Infect Immun. 1982 Jun;36(3):1102–1108. doi: 10.1128/iai.36.3.1102-1108.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Murphy B. R., Phelan M. A., Nelson D. L., Yarchoan R., Tierney E. L., Alling D. W., Chanock R. M. Hemagglutinin-specific enzyme-linked immunosorbent assay for antibodies to influenza A and B viruses. J Clin Microbiol. 1981 Mar;13(3):554–560. doi: 10.1128/jcm.13.3.554-560.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Murphy B. R., Tierney E. L., Barbour B. A., Yolken R. H., Alling D. W., Holley H. P., Jr, Mayner R. E., Chanock R. M. Use of the enzyme-linked immunosorbent assay to detect serum antibody responses of volunteers who received attenuated influenza A virus vaccines. Infect Immun. 1980 Aug;29(2):342–347. doi: 10.1128/iai.29.2.342-347.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Ogra P. L., Karzon D. T. Poliovirus antibody response in serum and nasal secretions following intranasal inoculation with inactivated poliovaccine. J Immunol. 1969 Jan;102(1):15–23. [PubMed] [Google Scholar]
  16. Ogra P. L., Kerr-Grant D., Umana G., Dzierba J., Weintraub D. Antibody response in serum and nasopharynx after naturally acquired and vaccine-induced infection with rubella virus. N Engl J Med. 1971 Dec 9;285(24):1333–1339. doi: 10.1056/NEJM197112092852401. [DOI] [PubMed] [Google Scholar]
  17. Phelan M. A., Mayner R. E., Bucher D. J., Ennis F. A. Purification of influenza virus glycoproteins for the preparation and standardization of immunological potency testing reagents. J Biol Stand. 1980;8(3):233–242. doi: 10.1016/s0092-1157(80)80039-4. [DOI] [PubMed] [Google Scholar]
  18. Plotkin S. A., Farquhar J. D., Ogra P. L. Immunologic properties of RA27-3 rubella virus vaccine. A comparison with strains presently licensed in the United States. JAMA. 1973 Aug 6;225(6):585–590. doi: 10.1001/jama.225.6.585. [DOI] [PubMed] [Google Scholar]
  19. Richman D. D., Murphy B. R., Chanock R. M., Gwaltney J. M., Jr, Douglas R. G., Betts R. F., Blacklow N. R., Rose F. B., Parrino T. A., Levine M. M. Temperature-sensitive mutants of influenza A virus. XII. Safety, antigenicity, transmissibility, and efficacy of influenza A/Udorn/72-ts-1[E] recombinant viruses in human adults. J Infect Dis. 1976 Dec;134(6):585–594. [PubMed] [Google Scholar]
  20. SCHLUEDERBERG A., KARELITZ S. SUPPRESSION OF MEASLES 19S ANTIBODY FORMATION AS EVIDENCE OF IMMUNITY. PRIMARY IMMUNE RESPONSE MASKED BY MATERIAL ANTIBODY. JAMA. 1965 Mar 29;191:1064–1066. doi: 10.1001/jama.1965.03080130024007. [DOI] [PubMed] [Google Scholar]
  21. Urquhart G. E. Serum IgM and IgA responses in influenza A infections. J Clin Pathol. 1974 Mar;27(3):198–201. doi: 10.1136/jcp.27.3.198. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Verhoeyen M., Fang R., Jou W. M., Devos R., Huylebroeck D., Saman E., Fiers W. Antigenic drift between the haemagglutinin of the Hong Kong influenza strains A/Aichi/2/68 and A/Victoria/3/75. Nature. 1980 Aug 21;286(5775):771–776. doi: 10.1038/286771a0. [DOI] [PubMed] [Google Scholar]
  23. Waldman R. H., Kasel J. A., Alford R. H., Mann J. J. Immunoglobulin classes of serum neutralizing antibody formed in response to infection with human A2 influenza virus. Proc Soc Exp Biol Med. 1967 May;125(1):316–321. doi: 10.3181/00379727-125-32081. [DOI] [PubMed] [Google Scholar]
  24. Webster R. G., Laver W. G., Air G. M., Schild G. C. Molecular mechanisms of variation in influenza viruses. Nature. 1982 Mar 11;296(5853):115–121. doi: 10.1038/296115a0. [DOI] [PubMed] [Google Scholar]
  25. Welliver R. C., Kaul T. N., Putnam T. I., Sun M., Riddlesberger K., Ogra P. L. The antibody response to primary and secondary infection with respiratory syncytial virus: kinetics of class-specific responses. J Pediatr. 1980 May;96(5):808–813. doi: 10.1016/s0022-3476(80)80547-6. [DOI] [PubMed] [Google Scholar]
  26. Zee Y. C., Osebold J. W., Dotson W. M. Antibody responses and interferon titers in the respiratory tracts of mice after aerosolized exposure to influenza virus. Infect Immun. 1979 Jul;25(1):202–207. doi: 10.1128/iai.25.1.202-207.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]

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