Skip to main content
NCBI home page
Infection and Immunity logoLink to Infection and Immunity
. 1983 Aug;41(2):644–649. doi: 10.1128/iai.41.2.644-649.1983

Siderophore production by Vibrio vulnificus.

L M Simpson, J D Oliver
PMCID: PMC264691  PMID: 6223882

Abstract

Previous studies in our laboratory, as well as clinical evidence, have suggested that increased iron levels in the host may be important in infections caused by the halophilic pathogen Vibrio vulnificus. To study iron acquisition, we induced siderophore production by growth in a low-iron medium, and biochemical testing indicated the production of both hydroxamate- and phenolate-type siderophores. The siderophores were extracted from growth filtrates with ethyl acetate (for phenolates) and phenol-chloroform-ether (for hydroxamates). These extracts enhanced the growth of V. vulnificus when the bacterium was grown in iron-limited medium. The ability of these siderophores to stimulate the growth of Salmonella typhimurium LT-2 enb-7 (a mutant deficient in the biosynthesis of enterochelin) and Arthrobacter flavescens JG-9 (a hydroxamate auxotroph) supported the conclusion that V. vulnificus produces both hydroxamate- and phenolate-type siderophores.

Full text

PDF
644

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Blake P. A., Merson M. H., Weaver R. E., Hollis D. G., Heublein P. C. Disease caused by a marine Vibrio. Clinical characteristics and epidemiology. N Engl J Med. 1979 Jan 4;300(1):1–5. doi: 10.1056/NEJM197901043000101. [DOI] [PubMed] [Google Scholar]
  2. Byers B. R., Powell M. V., Lankford C. E. Iron-chelating hydroxamic acid (schizokinen) active in initiation of cell division in Bacillus megaterium. J Bacteriol. 1967 Jan;93(1):286–294. doi: 10.1128/jb.93.1.286-294.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Estep M., Armstrong J. E., Van Baalen C. Evidence for the occurrence of specific iron (III)-binding compounds in near-shore marine ecosystems. Appl Microbiol. 1975 Aug;30(2):186–188. doi: 10.1128/am.30.2.186-188.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Ghosh H. K., Bowen T. E. Halophilic vibrios from human tissue infections on the pacific coast of Australia. Pathology. 1980 Jul;12(3):397–402. doi: 10.3109/00313028009077102. [DOI] [PubMed] [Google Scholar]
  5. Gibson F., Magrath D. I. The isolation and characterization of a hydroxamic acid (aerobactin) formed by Aerobacter aerogenes 62-I. Biochim Biophys Acta. 1969 Nov 18;192(2):175–184. doi: 10.1016/0304-4165(69)90353-5. [DOI] [PubMed] [Google Scholar]
  6. Holbein B. E. Iron-controlled infection with Neisseria meningitidis in mice. Infect Immun. 1980 Sep;29(3):886–891. doi: 10.1128/iai.29.3.886-891.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Kelly M. T., Avery D. M. Lactose-positive Vibrio in seawater: a cause of pneumonia and septicemia in a drowning victim. J Clin Microbiol. 1980 Mar;11(3):278–280. doi: 10.1128/jcm.11.3.278-280.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Kochan I., Wasynczuk J., McCabe M. A. Effects of injected iron and siderophores on infections in normal and immune mice. Infect Immun. 1978 Nov;22(2):560–567. doi: 10.1128/iai.22.2.560-567.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Leong J., Neilands J. B. Mechanisms of siderophore iron transport in enteric bacteria. J Bacteriol. 1976 May;126(2):823–830. doi: 10.1128/jb.126.2.823-830.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Luckey M., Pollack J. R., Wayne R., Ames B. N., Neilands J. B. Iron uptake in Salmonella typhimurium: utilization of exogenous siderochromes as iron carriers. J Bacteriol. 1972 Sep;111(3):731–738. doi: 10.1128/jb.111.3.731-738.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Mullis K. B., Pollack J. R., Neilands J. B. Structure of schizokinen, an iron-transport compound from Bacillus megaterium. Biochemistry. 1971 Dec 21;10(26):4894–4898. doi: 10.1021/bi00802a010. [DOI] [PubMed] [Google Scholar]
  12. Neilands J. B. Microbial iron compounds. Annu Rev Biochem. 1981;50:715–731. doi: 10.1146/annurev.bi.50.070181.003435. [DOI] [PubMed] [Google Scholar]
  13. Oliver J. D., Colwell R. R. Extractable lipids of gram-negative marine bacteria: phospholipid composition. J Bacteriol. 1973 Jun;114(3):897–908. doi: 10.1128/jb.114.3.897-908.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Payne S. M., Finkelstein R. A. Siderophore production by Vibrio cholerae. Infect Immun. 1978 Apr;20(1):310–311. doi: 10.1128/iai.20.1.310-311.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Payne S. M., Finkelstein R. A. The critical role of iron in host-bacterial interactions. J Clin Invest. 1978 Jun;61(6):1428–1440. doi: 10.1172/JCI109062. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Perry R. D., San Clemente C. L. Siderophore synthesis in Klebsiella pneumoniae and Shigella sonnei during iron deficiency. J Bacteriol. 1979 Dec;140(3):1129–1132. doi: 10.1128/jb.140.3.1129-1132.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Rogers H. J. Iron-Binding Catechols and Virulence in Escherichia coli. Infect Immun. 1973 Mar;7(3):445–456. doi: 10.1128/iai.7.3.445-456.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Sword C. P. Mechanisms of pathogenesis in Listeria monocytogenes infection. I. Influence of iron. J Bacteriol. 1966 Sep;92(3):536–542. doi: 10.1128/jb.92.3.536-542.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Van Tiel-Menkveld G. J., Mentjox-Vervuurt J. M., Oudega B., de Graaf F. K. Siderophore production by Enterobacter cloacae and a common receptor protein for the uptake of aerobactin and cloacin DF13. J Bacteriol. 1982 May;150(2):490–497. doi: 10.1128/jb.150.2.490-497.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Weinberg E. D. Iron and infection. Microbiol Rev. 1978 Mar;42(1):45–66. doi: 10.1128/mr.42.1.45-66.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Wright A. C., Simpson L. M., Oliver J. D. Role of iron in the pathogenesis of Vibrio vulnificus infections. Infect Immun. 1981 Nov;34(2):503–507. doi: 10.1128/iai.34.2.503-507.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Infection and Immunity are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES