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. Author manuscript; available in PMC: 2009 Dec 3.
Published in final edited form as: J Am Chem Soc. 2008 Dec 3;130(48):16338–16343. doi: 10.1021/ja806779e

Figure 2.

Figure 2

Lipid attachment through sortase-catalyzed transpeptidation. (a) Optimized procedure for lipid coupling followed by removal of His6-tagged proteins with Ni-NTA resin. (b) SDS-PAGE analysis of lipid-modified eGFP following transpeptidation and depletion of His6-tagged proteins. [Input = eGFP-LPETG-His6 in the absence of sortase and nucleophile, *Samples incubated with Ni-NTA resin and 300 mM imidazole to block binding of His6-tagged proteins].