Abstract
Germfree rats were challenged orally and intrarectally with spores of Clostridium tetani. Although C. tetani spores remained viable in the intestinal tract, they were unable to germinate. Germfree rats were then challenged orally with vegetative cells of C. tetani. Vegetative cells were able to colonize the intestinal tract, replicate, and produce toxin. Tetanus antitoxin, but no tetanus toxin, was detected in the sera of monoassociated rats.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Aranki A., Freter R. Use of anaerobic glove boxes for the cultivation of strictly anaerobic bacteria. Am J Clin Nutr. 1972 Dec;25(12):1329–1334. doi: 10.1093/ajcn/25.12.1329. [DOI] [PubMed] [Google Scholar]
- Arnon S. S., Midura T. F., Damus K., Wood R. M., Chin J. Intestinal infection and toxin production by Clostridium botulinum as one cause of sudden infant death syndrome. Lancet. 1978 Jun 17;1(8077):1273–1277. doi: 10.1016/s0140-6736(78)91264-3. [DOI] [PubMed] [Google Scholar]
- Burr D. H., Sugiyama H. Susceptibility to enteric botulinum colonization of antibiotic-treated adult mice. Infect Immun. 1982 Apr;36(1):103–106. doi: 10.1128/iai.36.1.103-106.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Moberg L. J., Sugiyama H. Microbial ecological basis of infant botulism as studied with germfree mice. Infect Immun. 1979 Aug;25(2):653–657. doi: 10.1128/iai.25.2.653-657.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Moberg L. J., Sugiyama H. The rat as an animal model for infant botulism. Infect Immun. 1980 Aug;29(2):819–821. doi: 10.1128/iai.29.2.819-821.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Sugiyama H., Mills D. C. Intraintestinal toxin in infant mice challenged intragastrically with Clostridium botulinum spores. Infect Immun. 1978 Jul;21(1):59–63. doi: 10.1128/iai.21.1.59-63.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Wells C. L., Sugiyama H., Bland S. E. Resistance of mice with limited intestinal flora to enteric colonization by Clostridium botulinum. J Infect Dis. 1982 Dec;146(6):791–796. doi: 10.1093/infdis/146.6.791. [DOI] [PubMed] [Google Scholar]
- Wilcke B. W., Jr, Midura T. F., Arnon S. S. Quantitative evidence of intestinal colonization by Clostridium botulinum in four cases of infant botulism. J Infect Dis. 1980 Apr;141(4):419–423. doi: 10.1093/infdis/141.4.419. [DOI] [PubMed] [Google Scholar]