Skip to main content
PMC home page
Infection and Immunity logoLink to Infection and Immunity
. 1983 Aug;41(2):869–871. doi: 10.1128/iai.41.2.869-871.1983

Antibody responses to virion polypeptides in gnotobiotic dogs infected with canine distemper virus.

J A Miele, S Krakowka
PMCID: PMC264726  PMID: 6874079

Abstract

A radioimmunoprecipitation-polyacrylamide gel electrophoresis technique was applied to sera from canine distemper virus-infected dogs. Sera from fatally infected dogs precipitated only the nucleoprotein, the matrix protein, and trace amounts of fusion glycoprotein. Sera from normal convalescent dogs precipitated all five major polypeptides. In contrast, sera from persistently infected dogs were characterized by a modest overall response compared with sera from convalescent dogs and by no or little response to the matrix and phosphorylated proteins until 5 to 7 weeks after infection.

Full text

PDF
869

Images in this article

870Image
on p.870

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Appel M. J. Pathogenesis of canine distemper. Am J Vet Res. 1969 Jul;30(7):1167–1182. [PubMed] [Google Scholar]
  2. Campbell J. J., Cosby S. L., Scott J. K., Rima B. K., Martin S. J., Appel M. A comparison of measles and canine distemper virus polypeptides. J Gen Virol. 1980 May;48(1):149–159. doi: 10.1099/0022-1317-48-1-149. [DOI] [PubMed] [Google Scholar]
  3. Choppin P. W., Scheid A. The role of viral glycoproteins in adsorption, penetration, and pathogenicity of viruses. Rev Infect Dis. 1980 Jan-Feb;2(1):40–61. doi: 10.1093/clinids/2.1.40. [DOI] [PubMed] [Google Scholar]
  4. Confer A. W., Kahn D. E., Koestner A., Krakowka S. Biological properties of a canine distemper virus isolate associated with demyelinating encephalomyelitis. Infect Immun. 1975 Apr;11(4):835–844. doi: 10.1128/iai.11.4.835-844.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Hall W. W., Choppin P. W. Evidence for lack of synthesis of the M polypeptide of measles virus in brain cells in subacute sclerosing panencephalitis. Virology. 1979 Dec;99(2):443–447. doi: 10.1016/0042-6822(79)90026-6. [DOI] [PubMed] [Google Scholar]
  6. Hall W. W., Choppin P. W. Measles-virus proteins in the brain tissue of patients with subacute sclerosing panencephalitis: absence of the M protein. N Engl J Med. 1981 May 7;304(19):1152–1155. doi: 10.1056/NEJM198105073041906. [DOI] [PubMed] [Google Scholar]
  7. Hall W. W., Imagawa D. T., Choppin P. W. Immunological evidence for the synthesis of all canine distemper virus polypeptides in chronic neurological diseases in dogs. Chronic distemper and old dog encephalitis differ from SSPE in man. Virology. 1979 Oct 15;98(1):283–287. doi: 10.1016/0042-6822(79)90550-6. [DOI] [PubMed] [Google Scholar]
  8. Hall W. W., Lamb R. A., Choppin P. W. Measles and subacute sclerosing panencephalitis virus proteins: lack of antibodies to the M protein in patients with subacute sclerosing panencephalitis. Proc Natl Acad Sci U S A. 1979 Apr;76(4):2047–2051. doi: 10.1073/pnas.76.4.2047. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Hall W. W., Lamb R. A., Choppin P. W. The polypeptides of canine distemper virus: synthesis in infected cells and relatedness to the polypeptides of other morbilliviruses. Virology. 1980 Jan 30;100(2):433–449. doi: 10.1016/0042-6822(80)90534-6. [DOI] [PubMed] [Google Scholar]
  10. Krakowka S., Higgins R. J., Koestner A. Canine distemper virus: review of structural and functional modulations in lymphoid tissues. Am J Vet Res. 1980 Feb;41(2):284–292. [PubMed] [Google Scholar]
  11. Krakowka S., Koestner A. Age-related susceptibility to infection with canine distemper virus in gnotobiotic dogs. J Infect Dis. 1976 Dec;134(6):629–632. doi: 10.1093/infdis/134.6.629. [DOI] [PubMed] [Google Scholar]
  12. Krakowka S., Long D., Mezza R., Mador R. A., Koestner A. Derivation and maintenance of gnotobiotic dogs. Lab Anim Sci. 1978 Jun;28(3):327–330. [PubMed] [Google Scholar]
  13. Krakowka S., McCullough B., Koestner A., Olsen R. Myelin-specific autoantibodies associated with central nervous system demyelination in canine distemper virus infection. Infect Immun. 1973 Nov;8(5):819–827. doi: 10.1128/iai.8.5.819-827.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Krakowka S., Olsen R., Confer A., Koestner A., McCullough B. Serologic response to canine distemper viral antigens in gnotobiotic dogs infected with canine distemper virus. J Infect Dis. 1975 Oct;132(4):384–392. doi: 10.1093/infdis/132.4.384. [DOI] [PubMed] [Google Scholar]
  15. Lin F. H., Thormar H. Absence of M protein in a cell-associated subacute sclerosing panencephalitis virus. Nature. 1980 Jun 12;285(5765):490–492. doi: 10.1038/285490a0. [DOI] [PubMed] [Google Scholar]
  16. McCullough B., Krakowka S., Koestner A. Experimental canine distemper virus-induced demyelination. Lab Invest. 1974 Sep;31(3):216–222. [PubMed] [Google Scholar]
  17. McCullough B., Krakowka S., Koestner A., Shadduck J. Demyelinating activity of canine distemper virus isolates in gnotobiotic dogs. J Infect Dis. 1974 Oct;130(4):343–350. doi: 10.1093/infdis/130.4.343. [DOI] [PubMed] [Google Scholar]
  18. Morgan E. M., Rapp F. Measles virus and its associated diseases. Bacteriol Rev. 1977 Sep;41(3):636–666. doi: 10.1128/br.41.3.636-666.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Norrby E., Orvell C., Vandvik B., Cherry J. D. Antibodies against measles virus polypeptides in different disease conditions. Infect Immun. 1981 Dec;34(3):718–724. doi: 10.1128/iai.34.3.718-724.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Orvell C. Structural polypeptides of canine distemper virus. Arch Virol. 1980;66(3):193–206. doi: 10.1007/BF01314733. [DOI] [PubMed] [Google Scholar]
  21. Stephenson J. R., ter Meulen V. Antigenic relationships between measles and canine distemper viruses: comparison of immune response in animals and humans to individual virus-specific polypeptides. Proc Natl Acad Sci U S A. 1979 Dec;76(12):6601–6605. doi: 10.1073/pnas.76.12.6601. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Summers B. A., Greisen H. A., Appel M. J. Possible initiation of viral encephalomyelitis in dogs by migrating lymphocytes infected with distemper virus. Lancet. 1978 Jul 22;2(8082):187–189. doi: 10.1016/s0140-6736(78)91924-4. [DOI] [PubMed] [Google Scholar]

Articles from Infection and Immunity are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES