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. 1983 Aug;41(2):872–875. doi: 10.1128/iai.41.2.872-875.1983

Production of monoclonal antibody against a glucosyltransferase of Streptococcus mutans 6715.

T Furuta, T Nisizawa, J Chiba, S Hamada
PMCID: PMC264727  PMID: 6223885

Abstract

A mouse hybrid cell line secreted monoclonal antibody which reacted specifically with Streptococcus mutans 6715 (serotype g) glucosyltransferase (GTase)-synthesizing water-insoluble glucan and inhibited with enzyme reaction. The antibody was cross-reactive with GTase of serotype d but not with GTase of other serotypes of S. mutans when an enzyme-linked immunosorbent assay was used.

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Selected References

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  1. Engvall E., Perlmann P. Enzyme-linked immunosorbent assay, Elisa. 3. Quantitation of specific antibodies by enzyme-labeled anti-immunoglobulin in antigen-coated tubes. J Immunol. 1972 Jul;109(1):129–135. [PubMed] [Google Scholar]
  2. Fukui K., Kokeguchi S., Kato K., Miyake Y., Nogami R., Moriyama T. Immunochemical properties of glucosyltransferases from Streptococcus mutans. Infect Immun. 1983 Feb;39(2):762–766. doi: 10.1128/iai.39.2.762-766.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Hamada S., Slade H. D. Adherence of serotype e Streptococcus mutans and the inhibitory effect of Lancefield group E and S mutans type e antiserum. J Dent Res. 1976 Apr;55(Spec No):C65–C74. doi: 10.1177/002203457605500328011. [DOI] [PubMed] [Google Scholar]
  4. Hamada S., Slade H. D. Biology, immunology, and cariogenicity of Streptococcus mutans. Microbiol Rev. 1980 Jun;44(2):331–384. doi: 10.1128/mr.44.2.331-384.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Hamada S., Tai S., Slade H. D. Serotype-dependent inhibition of glucan synthesis and cell adherence of Streptococcus mutans by antibody against glucosyltransferase of serotype e S. mutans. Microbiol Immunol. 1979;23(2):61–70. doi: 10.1111/j.1348-0421.1979.tb00442.x. [DOI] [PubMed] [Google Scholar]
  6. Kapitany R. A., Zebrowski E. J. A high resolution PAS stain for polyacrylamide gel electrophoresis. Anal Biochem. 1973 Dec;56(2):361–369. doi: 10.1016/0003-2697(73)90202-9. [DOI] [PubMed] [Google Scholar]
  7. Kuramitsu H., Ingersoll L. Immunological relationships between glucosyltransferases from Streptococcus mutans serotypes. Infect Immun. 1976 Sep;14(3):636–644. doi: 10.1128/iai.14.3.636-644.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Köhler G., Milstein C. Continuous cultures of fused cells secreting antibody of predefined specificity. Nature. 1975 Aug 7;256(5517):495–497. doi: 10.1038/256495a0. [DOI] [PubMed] [Google Scholar]
  9. Russell M. W., Challacombe S. J., Lehner T. Serum glucosyltransferase-inhibiting antibodies and dental caries in rhesus monkeys immunized against Streptococcus mutans. Immunology. 1976 May;30(5):619–627. [PMC free article] [PubMed] [Google Scholar]
  10. Russell R. R. Glycosyltransferases of Streptococcus mutans strain Ingbritt. Microbios. 1978;23(93-94):136–146. [PubMed] [Google Scholar]
  11. Smith D. J., Taubman M. A. Antigenic relatedness of glucosyltransferase enzymes from streptococcus mutans. Infect Immun. 1977 Jan;15(1):91–103. doi: 10.1128/iai.15.1.91-103.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Smith D. J., Taubman M. A., Ebersole J. L. Preparation of glucosyltransferase from Streptococcus mutans by elution from water-insoluble polysaccharide with a dissociating solvent. Infect Immun. 1979 Feb;23(2):446–452. doi: 10.1128/iai.23.2.446-452.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Towbin H., Staehelin T., Gordon J. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci U S A. 1979 Sep;76(9):4350–4354. doi: 10.1073/pnas.76.9.4350. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Tung A. S., Ju S. T., Sato S., Nisonoff A. Production of large amounts of antibodies in individual mice. J Immunol. 1976 Mar;116(3):676–681. [PubMed] [Google Scholar]

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