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. 1983 Jul;41(1):19–27. doi: 10.1128/iai.41.1.19-27.1983

Serology of oral Actinobacillus actinomycetemcomitans and serotype distribution in human periodontal disease.

J J Zambon, J Slots, R J Genco
PMCID: PMC264736  PMID: 6407997

Abstract

Actinobacillus actinomycetemcomitans from the human oral cavity was serologically characterized with rabbit antisera to the type strain NCTC 9710; a number of reference strains, including Y4, ATCC 29522, ATCC 29523, ATCC 29524, NCTC 9709; and our own isolates representative of each of 10 biotypes. Using immunoabsorbed antisera, we identified three distinct serotypes by immunodiffusion and indirect immunofluorescence. Serotype a was represented by ATCC 29523 and SUNYaB 75; serotype b was represented by ATCC 29522 and Y4; and serotype c was represented by NCTC 9710 and SUNYaB 67. Indirect immunofluorescence revealed no reaction between the three A. actinomycetemcomitans serotype-specific antisera and 62 strains representing 23 major oral bacterial species. Distinct from the serotype antigens were at least one A. actinomycetemcomitans species common antigen and an antigen shared with other Actinobacillus species, Haemophilus aphrophilus, and Haemophilus paraphrophilus. All serotype a A. actinomycetemcomitans strains failed to ferment xylose, whereas all serotype b organisms fermented xylose. Serotype c included xylose-positive as well as xylose-negative strains. A total of 301 isolates of A. actinomycetemcomitans from the oral cavity of 74 subjects were serologically categorized by indirect immunofluorescence with serotype-specific rabbit antisera. Each patient harbored only one serotype of A. actinomycetemcomitans. Fourteen healthy subjects, five diabetics, and seventeen adult periodontitis patients exhibited serotypes a and b in approximately equal frequency, whereas serotype c was found less frequently. In contrast, in 29 localized juvenile periodontitis patients, the incidence of serotype b was approximately two times higher than that of serotypes a or c, suggesting a particularly high periodontopathic potential of A. actinomycetemcomitans serotype b strains. In subjects infected with A. actinomycetemcomitans, serum antibodies were detected to the serotype antigens, indicating that these antigens may play a role in the pathogenesis of periodontal disease.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Baehni P., Tsai C. C., McArthur W. P., Hammond B. F., Taichman N. S. Interaction of inflammatory cells and oral microorganisms. VIII. Detection of leukotoxic activity of a plaque-derived gram-negative microorganism. Infect Immun. 1979 Apr;24(1):233–243. doi: 10.1128/iai.24.1.233-243.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Burgher L. W., Loomis G. W., Ware F. Systemic infection due to Actinobacillus actinomycetemcomitans. Am J Clin Pathol. 1973 Sep;60(3):412–415. doi: 10.1093/ajcp/60.3.412. [DOI] [PubMed] [Google Scholar]
  3. Ebersole J. L., Taubman M. A., Smith D. J., Genco R. J., Frey D. E. Human immune responses to oral micro-organisms. I. Association of localized juvenile periodontitis (LJP) with serum antibody responses to Actinobacillus actinomycetemcomitans. Clin Exp Immunol. 1982 Jan;47(1):43–52. [PMC free article] [PubMed] [Google Scholar]
  4. HEINRICH S., PULVERER G. Zur Aetiologie und Mikrobiologie der Aktinomykose. II. Definition und praktische Diagnostik des Actinobacillus actinomycetem-comitans. Zentralbl Bakteriol Orig. 1959 Jan;174(1-2):123–132. [PubMed] [Google Scholar]
  5. HEINRICH S., PULVERER G. [On the etiology and microbiology of actinomycosis. III. The pathogenic significance of Actinobacillus actinomycetem-comitans among the "bacterial symbionts" of Actinomyces israeli]. Zentralbl Bakteriol. 1959 Sep;176:91–101. [PubMed] [Google Scholar]
  6. Holt S. C., Tanner A. C., Socransky S. S. Morphology and ultrastructure of oral strains of Actinobacillus actinomycetemcomitans and Haemophilus aphrophilus. Infect Immun. 1980 Nov;30(2):588–600. doi: 10.1128/iai.30.2.588-600.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. KING E. O., TATUM H. W. Actinobacillus actinomycetemcomitans and Hemophilus aphrophilus. J Infect Dis. 1962 Sep-Oct;111:85–94. doi: 10.1093/infdis/111.2.85. [DOI] [PubMed] [Google Scholar]
  8. Kiley P., Holt S. C. Characterization of the lipopolysaccharide from Actinobacillus actinomycetemcomitans Y4 and N27. Infect Immun. 1980 Dec;30(3):862–873. doi: 10.1128/iai.30.3.862-873.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  10. Martin B. F., Derby B. M., Budzilovich G. N., Ransohoff J. Brain abscess due to Actinobacillus actinomycetemcomitans. Neurology. 1967 Sep;17(9):833–837. doi: 10.1212/wnl.17.9.833. [DOI] [PubMed] [Google Scholar]
  11. McCARTY M., LANCEFIELD R. C. Variation in the group-specific carbohydrate of group A streptococci. I. Immunochemical studies on the carbohydrates of variant strains. J Exp Med. 1955 Jul 1;102(1):11–28. doi: 10.1084/jem.102.1.11. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Mouton C., Reynolds H., Genco R. J. Combined micromanipulation, culture and immunofluorescent techniques for isolation of the coccal organisms comprising the "corn cob" configuration of human dental plaque. J Biol Buccale. 1977 Dec;5(4):321–332. [PubMed] [Google Scholar]
  13. Muhle I., Rau J., Ruskin J. Vertebral osteomyelitis due to Actinobacillus actinomycetemcomitans. JAMA. 1979 Apr 27;241(17):1824–1825. [PubMed] [Google Scholar]
  14. OUCHTERLONY O. Diffusion-in-gel methods for immunological analysis. Prog Allergy. 1958;5:1–78. [PubMed] [Google Scholar]
  15. Overholt B. F. Actinobacillus actinomycetemcomitans endocarditis. Arch Intern Med. 1966 Jan;117(1):99–102. [PubMed] [Google Scholar]
  16. PATHAK R. C., RISTIC M. Detection of an antibody to Actinobacillus lignieresi in infected human beings and the antigenic characterization of isolates of human and bovine origin. Am J Vet Res. 1962 Mar;23:310–314. [PubMed] [Google Scholar]
  17. Page M. I., King E. O. Infection due to Actinobacillus actinomycetemcomitans and Haemophilus aphrophilus. N Engl J Med. 1966 Jul 28;275(4):181–188. doi: 10.1056/NEJM196607282750403. [DOI] [PubMed] [Google Scholar]
  18. Pulverer G., Ko H. L. Serological studies on Actinobacillus actinomycetem-comitans. Appl Microbiol. 1972 Feb;23(2):207–210. doi: 10.1128/am.23.2.207-210.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Serra P., Tonato M. Subacute bacterial endocarditis due to Actinobacillus actinomycetemcomitans. Am J Med. 1969 Nov;47(5):809–812. doi: 10.1016/0002-9343(69)90174-0. [DOI] [PubMed] [Google Scholar]
  20. Slots J., Reynolds H. S., Genco R. J. Actinobacillus actinomycetemcomitans in human periodontal disease: a cross-sectional microbiological investigation. Infect Immun. 1980 Sep;29(3):1013–1020. doi: 10.1128/iai.29.3.1013-1020.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Slots J. Salient Biochemical Characters of Actinobacillus actinomycetemcomitans. Arch Microbiol. 1982 Feb;131(1):60–67. doi: 10.1007/BF00451500. [DOI] [PubMed] [Google Scholar]
  22. Slots J., Zambon J. J., Rosling B. G., Reynolds H. S., Christersson L. A., Genco R. J. Actinobacillus actinomycetemcomitans in human periodontal disease. Association, serology, leukotoxicity, and treatment. J Periodontal Res. 1982 Sep;17(5):447–448. doi: 10.1111/j.1600-0765.1982.tb02022.x. [DOI] [PubMed] [Google Scholar]
  23. Tanner A. C., Visconti R. A., Socransky S. S., Holt S. C. Classification and identification of Actinobacillus actinomycetemcomitans and haemophilus aphrophilus by cluster analysis and deoxyribonucleic acid hybridizations. J Periodontal Res. 1982 Nov;17(6):585–596. doi: 10.1111/j.1600-0765.1982.tb01180.x. [DOI] [PubMed] [Google Scholar]
  24. Townsend T. R., Gillenwater J. Y. Urinary tract infection due to Actinobacillus actinomycetemcomitans. JAMA. 1969 Oct 20;210(3):558–558. [PubMed] [Google Scholar]
  25. Vandepitte J., De Geest H., Jousten P. Subacute bacterial endocarditis due to Actinobacillus actinomycetemcomitans. Report of a case with a review of the literature. J Clin Pathol. 1977 Sep;30(9):842–846. doi: 10.1136/jcp.30.9.842. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. WETMORE P. W., THIEL J. F., HERMAN Y. F., HARR J. R. COMPARISON OF SELECTED ACTINOBACILLUS SPECIES WITH A HEMOLYTIC VARIETY OF ACTINOBACILLUS FROM IRRADIATED SWINE. J Infect Dis. 1963 Nov-Dec;113:186–194. doi: 10.1093/infdis/113.3.186. [DOI] [PubMed] [Google Scholar]
  27. ZAMENHOF S., LEIDY G., FITZGERALD P. L., ALEXANDER H. E., CHARGAFF E. Polyribophosphate, the type-specific substance of Hemophilus influenzae, type b. J Biol Chem. 1953 Aug;203(2):695–704. [PubMed] [Google Scholar]

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