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. Author manuscript; available in PMC: 2009 Nov 4.
Published in final edited form as: Biochemistry. 2008 Oct 9;47(44):11625–11636. doi: 10.1021/bi8015636

Figure 7.

Figure 7

Temperature dependence of metal uptake for active site variants of MnSOD. Apoprotein (50 μg/mL) in 20 mM MOPS pH 7 was analyzed by the fluorimetric metal uptake assay as described in the Methods. Kinetic time courses were analyzed to extract the gating fraction in the fast phase (A0/ATOT) for each temperature and the temperature dependence fit to a two-state thermal melting curve. (1) apo-MnSOD (Y34F), Tm = 24°C; (2) apo-MnSOD (Y34A), Tm = 28°C; (3) apo-MnSOD (H30A), Tm = 30°C; (4) apo-MnSOD (Y174F), Tm = 39°C; (5) apo-MnSOD (H171A), Tm = 43°C; (6) WT apo-MnSOD, Tm = 45°C.