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. 1983 Jul;41(1):244–250. doi: 10.1128/iai.41.1.244-250.1983

Babesia bovis: isolation of a protective antigen by using monoclonal antibodies.

I G Wright, M White, P D Tracey-Patte, R A Donaldson, B V Goodger, D J Waltisbuhl, D F Mahoney
PMCID: PMC264770  PMID: 6345394

Abstract

Three distinct monoclonal antibody-producing hybridomas have been produced against a partly purified protective fraction of Babesia bovis. All three stain the parasite or infected erythrocytes or both in precise and different manners when fluorescent-antibody techniques are used. The relevant antigens for each monoclonal antibody were isolated by immunoadsorption, their native molecular weights being 1.3 X 10(6), 180 X 10(3), and 44 X 10(3). Each antigen reacted in serological assays with homologous and heterologous bovine antisera to B. bovis. Susceptible splenectomized calves were immunized twice, 4 weeks apart, with the respective antigens and were challenged with virulent homologous organisms 2 weeks later. Strong protective immunity was induced by the antigen with a molecular weight of 44 X 10(3), but no significant protection was induced by either of the other two antigens.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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