Skip to main content
PMC home page
Infection and Immunity logoLink to Infection and Immunity
. 1983 Jul;41(1):426–429. doi: 10.1128/iai.41.1.426-429.1983

Detection of antibodies to human coronaviruses 229E and OC43 in the sera of multiple sclerosis patients and normal subjects.

D L Hovanec, T D Flanagan
PMCID: PMC264797  PMID: 6345398

Abstract

Sera collected from 90 multiple sclerosis patients and 148 age-matched normal subjects were examined for the presence of antibodies against human coronaviruses (HCV) 229E and OC43 by enzyme immunoassay (EIA). The results demonstrated no significant difference between the MS patients and the normal subjects in their antibody titer to HCV 229E and HCV OC43. Further analysis of these 238 sera indicated that a stronger EIA reaction was generally observed against HCV OC43 (mean EIA value at an optical density of 492 nm = 0.896) than against HCV 229E (mean EIA value at an optical density of 492 nm = 0.346).

Full text

PDF
426

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ADAMS J. M., IMAGAWA D. T. Measles antibodies in multiple sclerosis. Proc Soc Exp Biol Med. 1962 Dec;111:562–566. doi: 10.3181/00379727-111-27855. [DOI] [PubMed] [Google Scholar]
  2. Bradford M. M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976 May 7;72:248–254. doi: 10.1006/abio.1976.9999. [DOI] [PubMed] [Google Scholar]
  3. Burks J. S., DeVald B. L., Jankovsky L. D., Gerdes J. C. Two coronaviruses isolated from central nervous system tissue of two multiple sclerosis patients. Science. 1980 Aug 22;209(4459):933–934. doi: 10.1126/science.7403860. [DOI] [PubMed] [Google Scholar]
  4. Gerdes J. C., Klein I., DeVald B. L., Burks J. S. Coronavirus isolates SK and SD from multiple sclerosis patients are serologically related to murine coronaviruses A59 and JHM and human coronavirus OC43, but not to human coronavirus 229E. J Virol. 1981 Apr;38(1):231–238. doi: 10.1128/jvi.38.1.231-238.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Hendley J. O., Fishburne H. B., Gwaltney J. M., Jr Coronavirus infections in working adults. Eight-year study with 229 E and OC 43. Am Rev Respir Dis. 1972 May;105(5):805–811. doi: 10.1164/arrd.1972.105.5.805. [DOI] [PubMed] [Google Scholar]
  6. Kaye H. S., Marsh H. B., Dowdle W. R. Seroepidemiologic survey of coronavirus (strain OC 43) related infections in a children's population. Am J Epidemiol. 1971 Jul;94(1):43–49. doi: 10.1093/oxfordjournals.aje.a121293. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Kennedy D. A., Johnson-Lussenburg C. M. Isolation and morphology of the internal component of human coronavirus, strain 229E. Intervirology. 1975;6(4-5):197–206. doi: 10.1159/000149474. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Leinikki P. O., Holmes K. V., Shekarchi I., Iivanainen M., Madden D., Sever J. L. Coronavirus antibodies in patients with multiple sclerosis. Adv Exp Med Biol. 1981;142:323–326. doi: 10.1007/978-1-4757-0456-3_26. [DOI] [PubMed] [Google Scholar]
  9. Nagashima K., Wege H., Meyermann R., ter Meulen V. Corona virus induced subacute demyelinating encephalomyelitis in rats: a morphological analysis. Acta Neuropathol. 1978 Oct 13;44(1):63–70. doi: 10.1007/BF00691641. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Tanaka R., Iwasaki Y., Koprowski H. Intracisternal virus-like particles in brain of a multiple sclerosis patient. J Neurol Sci. 1976 May;28(1):121–126. doi: 10.1016/0022-510X(76)90053-8. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Infection and Immunity are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES