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. 1983 Apr;40(1):29–34. doi: 10.1128/iai.40.1.29-34.1983

Binding of human fibronectin to group A, C, and G streptococci.

E B Myhre, P Kuusela
PMCID: PMC264813  PMID: 6299959

Abstract

A total of 387 bacterial strains belonging to 35 species were tested in direct binding experiments for the uptake of purified radiolabeled human fibronectin. Positive binding was found in group A, C, and G streptococci and in Staphylococcus aureus. The group C streptococcal species Streptococcus equisimilis, Streptococcus zooepidemicus, Streptococcus equi, and Streptococcus dysgalactiae were uniformly reactive with fibronectin. Beta-hemolytic bovine group G streptococci showed the same degree of reactivity as human group G strains. In contrast, only 4 out of 15 alpha-hemolytic bovine group G strains were able to bind fibronectin. The uptake of fibronectin measured at room temperature with a human group G streptococcus was a slow, time-dependent process with maximum binding after approximately 1 h. Human polyclonal immunoglobulin G and serum albumin tested in inhibition experiments did not affect the fibronectin binding. Fibronectin seems, therefore, to interact with a surface component that is different from the specific binding sites previously described for human immunoglobulin G and serum albumin.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Beachey E. H., Simpson W. A. The adherence of group A streptococci to oropharyngeal cells: the lipoteichoic acid adhesin and fibronectin receptor. Infection. 1982;10(2):107–111. doi: 10.1007/BF01816738. [DOI] [PubMed] [Google Scholar]
  2. Christensen P., Kahlmeter G., Jonsson S., Kronvall G. New method for the serological grouping of Streptococci with specific antibodies adsorbed to protein A-containing staphylococci. Infect Immun. 1973 Jun;7(6):881–885. doi: 10.1128/iai.7.6.881-885.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Doran J. E., Raynor R. H. Fibronectin binding to protein A-containing staphylococci. Infect Immun. 1981 Sep;33(3):683–689. doi: 10.1128/iai.33.3.683-689.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Engvall E., Ruoslahti E. Binding of soluble form of fibroblast surface protein, fibronectin, to collagen. Int J Cancer. 1977 Jul 15;20(1):1–5. doi: 10.1002/ijc.2910200102. [DOI] [PubMed] [Google Scholar]
  5. Espersen F., Clemmensen I. Clumping of Staphylococcus aureus by human fibronectin. Acta Pathol Microbiol Scand B. 1981 Oct;89(5):317–321. doi: 10.1111/j.1699-0463.1981.tb00195_89b.x. [DOI] [PubMed] [Google Scholar]
  6. Espersen F., Clemmensen I. Isolation of a fibronectin-binding protein from Staphylococcus aureus. Infect Immun. 1982 Aug;37(2):526–531. doi: 10.1128/iai.37.2.526-531.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Harboe M., Fölling I. Recognition of two distinct groups of human IgM and IgA based on different binding to staphylococci. Scand J Immunol. 1974;3(4):471–482. doi: 10.1111/j.1365-3083.1974.tb01280.x. [DOI] [PubMed] [Google Scholar]
  8. Kronvall G., Myhre E. B., Björck L., Berggård I. Binding of aggregated human beta2-microglobulin to surface protein structure in group A, C, and G streptococci. Infect Immun. 1978 Oct;22(1):136–142. doi: 10.1128/iai.22.1.136-142.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Kronvall G., Schönbeck C., Myhre E. Fibrinogen binding structures in beta-hemolytic streptococci group A, C, and G. Comparisons with receptors for IgG and aggregated beta 2-microglobulin. Acta Pathol Microbiol Scand B. 1979 Oct;87(5):303–310. [PubMed] [Google Scholar]
  10. Kuusela P., Bång B. E., Hurme M., Mäkelä O. Reactivity of hybridoma antibodies with amniotic and plasma fibronectin. Scand J Immunol. 1980;12(4):331–337. doi: 10.1111/j.1365-3083.1980.tb00074.x. [DOI] [PubMed] [Google Scholar]
  11. Kuusela P. Fibronectin binds to Staphylococcus aureus. Nature. 1978 Dec 14;276(5689):718–720. doi: 10.1038/276718a0. [DOI] [PubMed] [Google Scholar]
  12. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  13. Lanser M. E., Saba T. M. Fibronectin as a co-factor necessary for optimal granulocyte phagocytosis of Staphylococcus aureus. J Reticuloendothel Soc. 1981 Nov;30(5):415–424. [PubMed] [Google Scholar]
  14. Marchalonis J. J. An enzymic method for the trace iodination of immunoglobulins and other proteins. Biochem J. 1969 Jun;113(2):299–305. doi: 10.1042/bj1130299. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Mosesson M. W., Chen A. B., Huseby R. M. The cold-insoluble globulin of human plasma: studies of its essential structural features. Biochim Biophys Acta. 1975 Apr 29;386(2):509–524. doi: 10.1016/0005-2795(75)90294-9. [DOI] [PubMed] [Google Scholar]
  16. Mosher D. F., Proctor R. A. Binding and factor XIIIa-mediated cross-linking of a 27-kilodalton fragment of fibronectin to Staphylococcus aureus. Science. 1980 Aug 22;209(4459):927–929. doi: 10.1126/science.7403857. [DOI] [PubMed] [Google Scholar]
  17. Myhre E. B., Holmberg O., Kronvall G. Immunoglobulin-binding structure on bovine group G streptococci different from type III Fc receptors on human group G streptococci. Infect Immun. 1979 Jan;23(1):1–7. doi: 10.1128/iai.23.1.1-7.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Myhre E. B., Kronvall G. Demonstration of a new type of immunoglobulin G receptor in Streptococcus zooepidemicus strains. Infect Immun. 1980 Mar;27(3):808–816. doi: 10.1128/iai.27.3.808-816.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Myhre E. B., Kronvall G. Demonstration of specific binding sites for human serum albumin in group C and G streptococci. Infect Immun. 1980 Jan;27(1):6–14. doi: 10.1128/iai.27.1.6-14.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Myhre E. B., Kronvall G. Heterogeneity of nonimmune immunoglobulin Fc reactivity among gram-positive cocci: description of three major types of receptors for human immunoglobulin G. Infect Immun. 1977 Sep;17(3):475–482. doi: 10.1128/iai.17.3.475-482.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Pearlstein E., Gold L. I., Garcia-Pardo A. Fibronectin: a review of its structure and biological activity. Mol Cell Biochem. 1980 Feb 8;29(2):103–128. doi: 10.1007/BF00220304. [DOI] [PubMed] [Google Scholar]
  22. Proctor R. A., Prendergast E., Mosher D. F. Fibronectin mediates attachment of Staphylococcus aureus to human neutrophils. Blood. 1982 Apr;59(4):681–687. [PubMed] [Google Scholar]
  23. Ruoslahti E., Engvall E., Hayman E. G. Fibronectin: current concepts of its structure and functions. Coll Relat Res. 1981;1(1):95–128. doi: 10.1016/s0174-173x(80)80011-2. [DOI] [PubMed] [Google Scholar]
  24. Simpson W. A., Beachey E. H. Adherence of group A streptococci to fibronectin on oral epithelial cells. Infect Immun. 1983 Jan;39(1):275–279. doi: 10.1128/iai.39.1.275-279.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Simpson W. A., Hasty D. L., Mason J. M., Beachey E. H. Fibronectin-mediated binding of group A streptococci to human polymorphonuclear leukocytes. Infect Immun. 1982 Aug;37(2):805–810. doi: 10.1128/iai.37.2.805-810.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Vaheri A., Mosher D. F. High molecular weight, cell surface-associated glycoprotein (fibronectin) lost in malignant transformation. Biochim Biophys Acta. 1978 Sep 18;516(1):1–25. doi: 10.1016/0304-419x(78)90002-1. [DOI] [PubMed] [Google Scholar]
  27. Verbrugh H. A., Peterson P. K., Smith D. E., Nguyen B. Y., Hoidal J. R., Wilkinson B. J., Verhoef J., Furcht L. T. Human fibronectin binding to staphylococcal surface protein and its relative inefficiency in promoting phagocytosis by human polymorphonuclear leukocytes, monocytes, and alveolar macrophages. Infect Immun. 1981 Sep;33(3):811–819. doi: 10.1128/iai.33.3.811-819.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Yamada K. M., Olden K. Fibronectins--adhesive glycoproteins of cell surface and blood. Nature. 1978 Sep 21;275(5677):179–184. doi: 10.1038/275179a0. [DOI] [PubMed] [Google Scholar]
  29. Zetter B. R., Daniels T. E., Quadra-White C., Greenspan J. S. LETS protein in normal and pathological human oral epithelium. J Dent Res. 1979 Jan;58(1):484–488. doi: 10.1177/00220345790580010401. [DOI] [PubMed] [Google Scholar]
  30. van de Water L., 3rd, Schroeder S., Crenshaw E. B., 3rd, Hynes R. O. Phagocytosis of gelatin-latex particles by a murine macrophage line is dependent on fibronectin and heparin. J Cell Biol. 1981 Jul;90(1):32–39. doi: 10.1083/jcb.90.1.32. [DOI] [PMC free article] [PubMed] [Google Scholar]

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