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. 1983 Apr;40(1):166–171. doi: 10.1128/iai.40.1.166-171.1983

Vwa+ phenotype of Yersinia enterocolitica.

R D Perry, R R Brubaker
PMCID: PMC264831  PMID: 6832830

Abstract

Expression of the Vwa+ phenotype of Yersinia pestis in vitro is known to reflect maximum induction of virulence (or V and W antigens) at 37 degrees C with concomitant restriction of cell division. Both phenomena are potentiated by 20 mM Mg2+ and prevented by cultivation at 26 or 37 degrees C with 2.5 mM Ca2+. We have now compared this classic plasmid-mediated phenotype with those of Vwa+ Yersinia pseudotuberculosis and Yersinia enterocolitica which, unlike Y. pestis, produce ancillary outer membrane peptides unrelated to the V and W antigens. All of 10 wild-type strains of Y. enterocolitica (serotypes O:3, O:4,32, O:8, O:9, O:15, and O:21) exhibited a nutritional requirement for Ca2+ at 37 degrees C and produced significant V antigen. Like Y. pseudotuberculosis, autoagglutination of Vwa+ Y. enterocolitica was dependent upon prior growth at 37 degrees C but was not influenced by Ca2+. Autoagglutination of Y. pestis was never observed. Resistance of Y. enterocolitica to 10% human serum was typically dependent upon prior growth at 37 degrees C, either with or without added Ca2+, and carriage of a Vwa plasmid. In contrast, serum resistance of Y. pseudotuberculosis was temperature but not plasmid dependent and that of Y. pestis was constitutive.

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Selected References

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  1. Acker G., Wartenberg K., Knapp W. Zuckerzusammensetzung des Lipopolysaccharids und Feinstruktur der äusseren Membran (Zellwand) bei Yersinia enterocolitica. Zentralbl Bakteriol A. 1980;247(2):229–240. [PubMed] [Google Scholar]
  2. Allen R. J., Scott G. K. Comparison of the effects of different lipopolysaccharides on the serum bactericidal reactions of two strains of Escherichia coli. Infect Immun. 1981 Feb;31(2):831–832. doi: 10.1128/iai.31.2.831-832.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. BACON G. A., BURROWS T. W. The basis of virulence in Pasteurella pestis: an antigen determining virulence. Br J Exp Pathol. 1956 Oct;37(5):481–493. [PMC free article] [PubMed] [Google Scholar]
  4. BRUBAKER R. R., SURGALLA M. J. THE EFFECT OF CA++ AND MG++ ON LYSIS, GROWTH, AND PRODUCTION OF VIRULENCE ANTIGENS BY PASTEURELLA PESTIS. J Infect Dis. 1964 Feb;114:13–25. doi: 10.1093/infdis/114.1.13. [DOI] [PubMed] [Google Scholar]
  5. BURROWS T. W., BACON G. A. The effects of loss of different virulence determinants on the virulence and immunogenicity of strains of Pasteurella pestis. Br J Exp Pathol. 1958 Jun;39(3):278–291. [PMC free article] [PubMed] [Google Scholar]
  6. BURROWS T. W., JACKSON S. The pigmentation of Pasteurella pestis on a defined medium containing haemin. Br J Exp Pathol. 1956 Dec;37(6):570–576. [PMC free article] [PubMed] [Google Scholar]
  7. BURROWS T. W. VIRULENCE OF PASTEURELLA PESTIS AND IMMUNITY TO PLAGUE. Ergeb Mikrobiol Immunitatsforsch Exp Ther. 1963;37:59–113. doi: 10.1007/978-3-662-36742-1_2. [DOI] [PubMed] [Google Scholar]
  8. Ben-Gurion R., Shafferman A. Essential virulence determinants of different Yersinia species are carried on a common plasmid. Plasmid. 1981 Mar;5(2):183–187. doi: 10.1016/0147-619x(81)90019-6. [DOI] [PubMed] [Google Scholar]
  9. Brubaker R. R. Growth of Pasteurella pseudotuberculosis in simulated intracellular and extracellular environments. J Infect Dis. 1967 Dec;117(5):403–417. doi: 10.1093/infdis/117.5.403. [DOI] [PubMed] [Google Scholar]
  10. Brubaker R. R. Interconversion of Purine Mononucleotides in Pasteurella pestis. Infect Immun. 1970 May;1(5):446–454. doi: 10.1128/iai.1.5.446-454.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Brubaker R. R. The genus Yersinia: biochemistry and genetics of virulence. Curr Top Microbiol Immunol. 1972;57:111–158. doi: 10.1007/978-3-642-65297-4_4. [DOI] [PubMed] [Google Scholar]
  12. Bölin I., Norlander L., Wolf-Watz H. Temperature-inducible outer membrane protein of Yersinia pseudotuberculosis and Yersinia enterocolitica is associated with the virulence plasmid. Infect Immun. 1982 Aug;37(2):506–512. doi: 10.1128/iai.37.2.506-512.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. CRUMPTON M. J., DAVIES D. A. An antigenic analysis of Pasteurella pestis by diffusion of antigens and antibodies in agar. Proc R Soc Lond B Biol Sci. 1956 Mar 27;144(918):109–134. doi: 10.1098/rspb.1956.0021. [DOI] [PubMed] [Google Scholar]
  14. Carter P. B., Collins F. M. Experimental Yersinia enterocolitica infection in mice: kinetics of growth. Infect Immun. 1974 May;9(5):851–857. doi: 10.1128/iai.9.5.851-857.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Carter P. B., Zahorchak R. J., Brubaker R. R. Plague virulence antigens from Yersinia enterocolitica. Infect Immun. 1980 May;28(2):638–640. doi: 10.1128/iai.28.2.638-640.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Charnetzky W. T., Brubaker R. R. RNA synthesis in Yersinia pestis during growth restriction in calcium-deficient medium. J Bacteriol. 1982 Mar;149(3):1089–1095. doi: 10.1128/jb.149.3.1089-1095.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. DAVIES D. A. Dideoxysugars of Pasteurella pseudotuberculosis-specific polysaccharides, and the occurrence of ascarylose. Nature. 1961 Jul 1;191:43–44. doi: 10.1038/191043a0. [DOI] [PubMed] [Google Scholar]
  18. DEVIGNAT R. Variétés de l'espèce Pasteurella pestis; nouvelle hypothèse. Bull World Health Organ. 1951;4(2):247–263. [PMC free article] [PubMed] [Google Scholar]
  19. Doyle M. P., Hugdahl M. B., Chang M. T., Beery J. T. Serological relatedness of mouse-virulent Yersinia enterocolitica. Infect Immun. 1982 Sep;37(3):1234–1240. doi: 10.1128/iai.37.3.1234-1240.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Ferber D. M., Brubaker R. R. Plasmids in Yersinia pestis. Infect Immun. 1981 Feb;31(2):839–841. doi: 10.1128/iai.31.2.839-841.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Ferber D. M., Fowler J. M., Brubaker R. R. Mutations to tolerance and resistance to pesticin and colicins in Escherichia coli phi. J Bacteriol. 1981 May;146(2):506–511. doi: 10.1128/jb.146.2.506-511.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Gemski P., Lazere J. R., Casey T. Plasmid associated with pathogenicity and calcium dependency of Yersinia enterocolitica. Infect Immun. 1980 Feb;27(2):682–685. doi: 10.1128/iai.27.2.682-685.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Gemski P., Lazere J. R., Casey T., Wohlhieter J. A. Presence of a virulence-associated plasmid in Yersinia pseudotuberculosis. Infect Immun. 1980 Jun;28(3):1044–1047. doi: 10.1128/iai.28.3.1044-1047.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. HIGUCHI K., CARLIN C. E. Studies on the nutrition and physiology of Pasteurella pestis. II. A defined medium for the growth of Pasteurella pestis. J Bacteriol. 1958 Apr;75(4):409–413. doi: 10.1128/jb.75.4.409-413.1958. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. HIGUCHI K., SMITH J. L. Studies on the nutrition and physiology of Pasteurella pestis. VI. A differential plating medium for the estimation of the mutation rate to avirulence. J Bacteriol. 1961 Apr;81:605–608. doi: 10.1128/jb.81.4.605-608.1961. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Harrison D. N., Laird W., Robinson D. M., Cavanaugh D. C. Commonality of a virulence factor among Yersinia species. J Infect Dis. 1980 Mar;141(3):413–413. doi: 10.1093/infdis/141.3.413. [DOI] [PubMed] [Google Scholar]
  27. Hellmann E., Schenk I. Beeinflusst die Bebrütungstemperatur den Endotoxingehalt von yersinia enterocolitica? Zentralbl Bakteriol Mikrobiol Hyg A. 1982 Apr;251(4):529–536. [PubMed] [Google Scholar]
  28. Hoffman J., Lindberg B., Brubaker R. R. Structural studies of the O-specific side-chains of the lipopolysaccharide from Yersinia enterocolitica Ye 128. Carbohydr Res. 1980 Jan 1;78(1):212–214. doi: 10.1016/s0008-6215(00)83675-7. [DOI] [PubMed] [Google Scholar]
  29. Hu P. C., Brubaker R. R. Characterization of pesticin. Separation of antibacterial activities. J Biol Chem. 1974 Aug 10;249(15):4749–4753. [PubMed] [Google Scholar]
  30. Kay B. A., Wachsmuth K., Gemski P. New virulence-associated plasmid in Yersinia enterocolitica. J Clin Microbiol. 1982 Jun;15(6):1161–1163. doi: 10.1128/jcm.15.6.1161-1163.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  31. LAWTON W. D., ERDMAN R. L., SURGALLA M. J. BIOSYNTHESIS AND PURIFICATION OF V AND W ANTIGEN IN PASTEURELLA PESTIS. J Immunol. 1963 Aug;91:179–184. doi: 10.21236/ad0299868. [DOI] [PubMed] [Google Scholar]
  32. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  33. Laird W. J., Cavanaugh D. C. Correlation of autoagglutination and virulence of yersiniae. J Clin Microbiol. 1980 Apr;11(4):430–432. doi: 10.1128/jcm.11.4.430-432.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  34. Morrison D. C., Kline L. F. Activation of the classical and properdin pathways of complement by bacterial lipopolysaccharides (LPS). J Immunol. 1977 Jan;118(1):362–368. [PubMed] [Google Scholar]
  35. Pai C. H., DeStephano L. Serum resistance associated with virulence in Yersinia enterocolitica. Infect Immun. 1982 Feb;35(2):605–611. doi: 10.1128/iai.35.2.605-611.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  36. Portnoy D. A., Moseley S. L., Falkow S. Characterization of plasmids and plasmid-associated determinants of Yersinia enterocolitica pathogenesis. Infect Immun. 1981 Feb;31(2):775–782. doi: 10.1128/iai.31.2.775-782.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  37. Richardson M., Harkness T. K. Intracellular Pasteurella pseudotuberculosis: Multiplication in Cultured Spleen and Kidney Cells. Infect Immun. 1970 Nov;2(5):631–639. doi: 10.1128/iai.2.5.631-639.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  38. Samuelsson K., Lindberg B., Brubaker R. R. Structure of O-specific side chains of lipopolysaccharides from Yersinia pseudotuberculosis. J Bacteriol. 1974 Mar;117(3):1010–1016. doi: 10.1128/jb.117.3.1010-1016.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  39. Schiemann D. A., Devenish J. A. Relationship of HeLa cell infectivity to biochemical, serological, and virulence characteristics of Yersinia enterocolitica. Infect Immun. 1982 Feb;35(2):497–506. doi: 10.1128/iai.35.2.497-506.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  40. Straley S. C., Brubaker R. R. Cytoplasmic and membrane proteins of yersiniae cultivated under conditions simulating mammalian intracellular environment. Proc Natl Acad Sci U S A. 1981 Feb;78(2):1224–1228. doi: 10.1073/pnas.78.2.1224. [DOI] [PMC free article] [PubMed] [Google Scholar]
  41. Straley S. C., Brubaker R. R. Localization in Yersinia pestis of peptides associated with virulence. Infect Immun. 1982 Apr;36(1):129–135. doi: 10.1128/iai.36.1.129-135.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  42. Tee G. L., Scott G. K. Analysis of outer membrane components of Escherichia coli ML308 225 and of a serum-resistant mutant. Infect Immun. 1980 May;28(2):387–392. doi: 10.1128/iai.28.2.387-392.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  43. Vesikari T., Nurmi T., Mäki M., Skurnik M., Sundqvist C., Granfors K., Grönroos P. Plasmids in Yersinia enterocolitica serotypes O:3 and O:9: correlation with epithelial cell adherence in vitro. Infect Immun. 1981 Sep;33(3):870–876. doi: 10.1128/iai.33.3.870-876.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  44. Wartenberg K., Lysy J., Knapp W. On the sugar content of the lipopolysaccharides of the various strains known as Yersinia enterocolitica. Zentralbl Bakteriol Orig A. 1975;230(3):361–366. [PubMed] [Google Scholar]
  45. Wauters G., Le Minor L., Chalon A. M., Lassen J. Supplément au schéma antigénique de "Yersinia enterocolitica". Ann Inst Pasteur (Paris) 1972 May;122(5):951–956. [PubMed] [Google Scholar]
  46. Zahorchak R. J., Brubaker R. R. Effect of exogenous nucleotides on Ca2+ dependence and V antigen synthesis in Yersinia pestis. Infect Immun. 1982 Dec;38(3):953–959. doi: 10.1128/iai.38.3.953-959.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  47. Zahorchak R. J., Charnetzky W. T., Little R. V., Brubaker R. R. Consequences of Ca2+ deficiency on macromolecular synthesis and adenylate energy charge in Yersinia pestis. J Bacteriol. 1979 Sep;139(3):792–799. doi: 10.1128/jb.139.3.792-799.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]

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