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. 1983 Apr;40(1):219–224. doi: 10.1128/iai.40.1.219-224.1983

Granulocyte phagocytosis of Streptococcus pneumoniae in properdin-deficient serum.

J H Braconier, H Odeberg, A G Sjöholm
PMCID: PMC264839  PMID: 6403465

Abstract

Properdin (P)-deficient human serum containing type-specific anticapsular antibodies and having an intact classical pathway of complement did not support efficient granulocyte phagocytosis of Streptococcus pneumoniae serotypes 6A, 14, 19F, 23F, or 35 in an opsonophagocytic assay. Compared with pooled control serum, the difference was most pronounced for serotypes 14 and 23F and at a final serum concentration of 16%. The reduced phagocytic killing of S. pneumoniae serotype 23F in P-deficient serum was due rather to defective opsonization than to impaired intracellular killing. The uptake of C3 by the serotype 23F strain was found to be low in P-deficient serum. The addition of native P promoted C3 fixation as well as opsonization. The activity of P was abolished by heat treatment (56 degrees C, 30 min). Experiments with Mg EGTA to block C1 activation suggested that serotype 23F pneumococci were more dependent on the classical pathway for opsonization than were serotype 35 pneumococci, which appear to be partly opsonized through the alternative pathway alone.

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Selected References

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  1. Agnello V. Complement deficiency states. Medicine (Baltimore) 1978 Jan;57(1):1–23. doi: 10.1097/00005792-197801000-00001. [DOI] [PubMed] [Google Scholar]
  2. Braconier J. H., Odeberg H. Granulocyte phagocytosis and killing virulent and avirulent serotypes of Streptococcus pneumoniae. J Lab Clin Med. 1982 Aug;100(2):279–287. [PubMed] [Google Scholar]
  3. Brown E. J., Hosea S. W., Hammer C. H., Burch C. G., Frank M. M. A quantitative analysis of the interactions of antipneumococcal antibody and complement in experimental pneumococcal bacteremia. J Clin Invest. 1982 Jan;69(1):85–98. doi: 10.1172/JCI110444. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Davis C. A., Forristal J. Partial properdin deficiency. J Lab Clin Med. 1980 Oct;96(4):633–639. [PubMed] [Google Scholar]
  5. Edwards K. M., Gewurz H., Lint T. F., Mold C. A role for C-reactive protein in the complement-mediated stimulation of human neutrophils by type 27 Streptococcus pneumoniae. J Immunol. 1982 Jun;128(6):2493–2496. [PubMed] [Google Scholar]
  6. Ehlenberger A. G., Nussenzweig V. The role of membrane receptors for C3b and C3d in phagocytosis. J Exp Med. 1977 Feb 1;145(2):357–371. doi: 10.1084/jem.145.2.357. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Fearon D. T., Austen K. F. Current concepts in immunology: the alternative pathway of complement--a system for host resistance to microbial infection. N Engl J Med. 1980 Jul 31;303(5):259–263. doi: 10.1056/NEJM198007313030505. [DOI] [PubMed] [Google Scholar]
  8. Fine D. P., Marney S. R., Jr, Colley D. G., Sergent J. S., Des Prez R. M. C3 shunt activation in human serum chelated with EGTA. J Immunol. 1972 Oct;109(4):807–809. [PubMed] [Google Scholar]
  9. Fine D. P. Pneumococcal type-associated variability in alternate complement pathway activation. Infect Immun. 1975 Oct;12(4):772–778. doi: 10.1128/iai.12.4.772-778.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Ganrot P. O. Crossed immunoelectrophoresis. Scand J Clin Lab Invest Suppl. 1972;124:39–47. doi: 10.3109/00365517209102749. [DOI] [PubMed] [Google Scholar]
  11. Gardner S. E., Anderson D. C., Webb B. J., Stitzel A. E., Edwards M. S., Spitzer R. E., Baker C. J. Evaluation of Streptococcus pneumoniae type XIV opsonins by phagocytosis-associated chemiluminescence and a bactericidal assay. Infect Immun. 1982 Mar;35(3):800–808. doi: 10.1128/iai.35.3.800-808.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Giebink G. S., Verhoef J., Peterson P. K., Quie P. G. Opsonic requirements for phagocytosis of Streptococcus pneumoniae types VI, XVIII, XXIII, and XXV. Infect Immun. 1977 Nov;18(2):291–297. doi: 10.1128/iai.18.2.291-297.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Guckian J. C., Christensen G. D., Schweinle J. E., Fine D. P. Opsonization of pneumococci. I. Heat-labile serum activity other than complement is required for killing by human polymorphonuclear leukocytes. J Immunol. 1981 Oct;127(4):1659–1665. [PubMed] [Google Scholar]
  14. Götze O., Medicus R. G., Müller-Eberhard H. J. Alternative pathway of complement: nonenzymatic, reversible transition of precursor to active properdin. J Immunol. 1977 Feb;118(2):525–532. [PubMed] [Google Scholar]
  15. Leijh P. C., van den Barselaar M. T., van Zwet T. L., Daha M. R., van Furth R. Requirement of extracellular complement and immunoglobulin for intracellular killing of micro-organisms by human monocytes. J Clin Invest. 1979 Apr;63(4):772–784. doi: 10.1172/JCI109362. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Matthay K. K., Mentzer W. C., Wara D. W., Preisler H. K., Lameris N. B., Ammann A. J. Evaluation of the opsonic requirements for phagocytosis of Streptococcus pneumoniae serotypes VII, XIV, and XIX by chemiluminescence assay. Infect Immun. 1981 Jan;31(1):228–235. doi: 10.1128/iai.31.1.228-235.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. McConahey P. J., Dixon F. J. A method of trace iodination of proteins for immunologic studies. Int Arch Allergy Appl Immunol. 1966;29(2):185–189. doi: 10.1159/000229699. [DOI] [PubMed] [Google Scholar]
  18. Minta J. O., Vetvutanapibul W. The effects of heat, pH and chemical agents on the functional activity of properdin. Immunochemistry. 1978 Feb;15(2):93–96. doi: 10.1016/0161-5890(78)90048-2. [DOI] [PubMed] [Google Scholar]
  19. Newman S. L., Vogler L. B., Feigin R. D., Johnston R. B., Jr Recurrent septicemia associated with congenital deficiency of C2 and partial deficiency of factor B and the alternative complement pathway. N Engl J Med. 1978 Aug 10;299(6):290–292. doi: 10.1056/NEJM197808102990606. [DOI] [PubMed] [Google Scholar]
  20. Odeberg H., Olofsson T., Olsson I. Granulocyte function in chronic granulocytic leukaemia. I. Bactericidal and metabolic capabilities during phagocytosis in isolated granulocytes. Br J Haematol. 1975 Mar;29(3):427–441. doi: 10.1111/j.1365-2141.1975.tb01840.x. [DOI] [PubMed] [Google Scholar]
  21. Pedersen F. K., Henrichsen J. Detection of antibodies to pneumococcal capsular polysaccharides by enzyme-linked immunosorbent assay. J Clin Microbiol. 1982 Mar;15(3):372–378. doi: 10.1128/jcm.15.3.372-378.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Prellner K. Complement activation by pneumococci associated with acute otitis media. Acta Pathol Microbiol Scand C. 1979 Jun;87C(3):213–216. [PubMed] [Google Scholar]
  23. Rosen F. S., Janeway C. A. The gamma globulins. 3. The antibody deficiency syndromes. N Engl J Med. 1966 Sep 29;275(13):709–contd. doi: 10.1056/NEJM196609292751307. [DOI] [PubMed] [Google Scholar]
  24. Sjöholm A. G., Braconier J. H., Söderström C. Properdin deficiency in a family with fulminant meningococcal infections. Clin Exp Immunol. 1982 Nov;50(2):291–297. [PMC free article] [PubMed] [Google Scholar]
  25. Stephens C. G., Williams R. C., Jr, Reed W. P. Classical and alternative complement pathway activation by pneumococci. Infect Immun. 1977 Aug;17(2):296–302. doi: 10.1128/iai.17.2.296-302.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Verbrugh H. A., van Dijk W. C., van Erne M. E., Peters R., Peterson P. K., Verhoef J. Quantitation of the third component of human complement attached to the surface of opsonized bacteria: opsonin-deficient sera and phagocytosis-resistant strains. Infect Immun. 1979 Dec;26(3):808–814. doi: 10.1128/iai.26.3.808-814.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Verhoef J., Peterson P. K., Quie P. G. Kinetics of staphylococcal opsonization, attachment, ingestion and killing by human polymorphonuclear leukocytes: a quantitative assay using [3H]thymidine labeled bacteria. J Immunol Methods. 1977;14(3-4):303–311. doi: 10.1016/0022-1759(77)90141-7. [DOI] [PubMed] [Google Scholar]
  28. Winkelstein J. A., Bocchini J. A., Jr, Schiffman G. The role of the capsular polysaccharide in the activation of the alternative pathway by the pneumococcus. J Immunol. 1976 Feb;116(2):367–370. [PubMed] [Google Scholar]
  29. Winkelstein J. A. Opsonins: their function, identity, and clinical significance. J Pediatr. 1973 May;82(5):747–753. doi: 10.1016/s0022-3476(73)80062-9. [DOI] [PubMed] [Google Scholar]

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