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. 1983 Apr;40(1):292–298. doi: 10.1128/iai.40.1.292-298.1983

Biological properties of rabbit antibodies to a surface antigen of Rickettsia rickettsii.

R L Anacker, R N Philip, E Casper, W J Todd, R E Mann, M R Johnston, C J Nauck
PMCID: PMC264847  PMID: 6403467

Abstract

Because of the potential significance of external components of the obligate intracellular parasite Rickettsia rickettsii in host-parasite interactions, we have begun the first phase of a study to isolate and characterize surface antigens of this organism. An antiserum to a rickettsial surface component was obtained from rabbits inoculated with immune precipitates prepared by crossed immunoelectrophoresis of Triton X-100 extracts of R. rickettsii strain R. This antiserum (i) protected guinea pigs inoculated with 10,000 guinea pig 50% infectious doses of R. rickettsii against fever, (ii) prevented death of mice challenged with 2 50% lethal doses of R. rickettsii, and (iii) reacted in the microimmunofluorescence test with 9 of 13 spotted fever group serotypes tested. The location of this antigen on the rickettsial surface was demonstrated by immunoelectron microscopy with ferritin-labeled antibodies.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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