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. 1983 Apr;40(1):389–397. doi: 10.1128/iai.40.1.389-397.1983

Application of monoclonal antibodies to detect intraocular mycoplasma antigens in Mycoplasma arthritidis-infected Sprague-Dawley rats.

C E Thirkill, D Y Song, D S Gregerson
PMCID: PMC264859  PMID: 6339406

Abstract

Sprague-Dawley rats infected with Mycoplasma arthritidis by tail vein inoculation develop extensive disseminated joint inflammation, frequently accompanied by conjunctivitis and anterior uveitis. The intraocular inflammation is apparently directed at mycoplasmas localized within the stroma of the ciliary body, which have been detected with monoclonal antibodies by indirect immunofluorescence. The monoclonal antibodies are directed against an antigenic determinant on the enzyme arginine deiminase isolated from M. arthritidis, but they do not react with the same enzyme derived from Mycoplasma hominis. The antigen bound by the monoclonal antibodies can also be detected by immunofluorescence in M. arthritidis-infected tissue cultures and is not lost after glutaraldehyde fixation or paraffin-embedding procedures. The value in the application of monoclonal antibodies reactive with arginine deiminase lies in the fact that although this enzyme may be found in mycoplasmas and several other species of bacteria it is not a normal constituent of mammalian tissues.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BARTHOLOMEW L. E. ISOLATION AND CHARACTERIZATION OF MYCOPLASMAS (PPLO) FROM PATIENTS WITH RHEUMATOID ARTHRITIS, SYSTEMIC LUPUS ERYTHEMATOSUS AND REITER'S SYNDROME. Arthritis Rheum. 1965 Jun;8:376–388. doi: 10.1002/art.1780080306. [DOI] [PubMed] [Google Scholar]
  2. Bosman F. T., Nieuwenhuijzen Kruseman A. C. Clinical applications of the enzyme labeled antibody method. Immunoperoxidase methods in diagnostic histopathology. J Histochem Cytochem. 1979 Aug;27(8):1140–1147. doi: 10.1177/27.8.90075. [DOI] [PubMed] [Google Scholar]
  3. Bradford M. M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976 May 7;72:248–254. doi: 10.1016/0003-2697(76)90527-3. [DOI] [PubMed] [Google Scholar]
  4. Cole B. C., Miller M. L., Ward J. R. A comparative study on the virulence of Mycoplasma arthritidis and "Mycoplasma hominis, type 2" strains in rats. Proc Soc Exp Biol Med. 1967 Jan;124(1):103–107. doi: 10.3181/00379727-124-31676. [DOI] [PubMed] [Google Scholar]
  5. Frackelton A. R., Jr, Rotman B. Functional diversity of antibodies elicited by bacterial beta-D-galactosidase. Monoclonal activating, inactivating, protecting, and null antibodies to normal enzyme. J Biol Chem. 1980 Jun 10;255(11):5286–5290. [PubMed] [Google Scholar]
  6. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  7. Mårdh P. A., Nilsson F. J., Bjelle A. Mycoplasmas and bacteria in synovial fluid from patients with arthritis. Ann Rheum Dis. 1973 Jul;32(4):319–325. doi: 10.1136/ard.32.4.319. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. OATES J. K., WHITTINGTON M. J., WILKINSON A. E. A note on the results of cultural and serological tests for pleuropneumonia-like organisms in Reiter's disease. Br J Vener Dis. 1959 Sep;35:184–186. doi: 10.1136/sti.35.3.184. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Stjernschantz J., Gregerson D., Bausher L., Sears M. Enzyme-linked immunosorbent assay of substance P: a study in the eye. J Neurochem. 1982 May;38(5):1323–1328. doi: 10.1111/j.1471-4159.1982.tb07908.x. [DOI] [PubMed] [Google Scholar]
  10. Thirkill C. E., Gregerson D. S. Mycoplasma arthritidis-induced ocular inflammatory disease. Infect Immun. 1982 May;36(2):775–781. doi: 10.1128/iai.36.2.775-781.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Weickmann J. L., Fahrney D. E. Arginine deiminase from Mycoplasma arthritidis. Evidence for multiple forms. J Biol Chem. 1977 Apr 25;252(8):2615–2620. [PubMed] [Google Scholar]
  12. Weickmann J. L., Himmel M. E., Squire P. G., Fahrney D. E. Arginine deiminase from Mycoplasma arthritidis. Properties of the enzyme from log phase cultures. J Biol Chem. 1978 Sep 10;253(17):6010–6015. [PubMed] [Google Scholar]
  13. de StGroth S. F., Scheidegger D. Production of monoclonal antibodies: strategy and tactics. J Immunol Methods. 1980;35(1-2):1–21. doi: 10.1016/0022-1759(80)90146-5. [DOI] [PubMed] [Google Scholar]

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