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. Author manuscript; available in PMC: 2009 Dec 10.
Published in final edited form as: Exp Cell Res. 2008 Oct 7;314(20):3724–3736. doi: 10.1016/j.yexcr.2008.09.019

Fig. 2. JNK activation is inhibited in T. gondii-infected HeLa cells following exposure to TNFα.

Fig. 2

(A) A representative immunoblot of uninfected and T. gondii infected HeLa cells following exposure to TNFα and harvested at the time points indicated. JNK-phosphorylation (PJNK) occurs transiently, peaking at 30 minutes post exposure. This response is markedly inhibited in cells infected with T. gondii although the kinetics of the response do not appear to be impacted. Total JNK (JNK) and calnexin serve as loading controls. (B) Densitometric analysis representing the level of phospho-p54 JNK relative to total p54 JNK in four independent experiments depicts the range of values inherent in such experiments. Relative phospho-p54 levels in uninfected cells (open circle) versus infected cells (diamond) confirm a marked inhibition of mean phospho-p54 JNK accumulation (horizontal line) in parasite infected cells relative to uninfected cells. Densitometric analysis was performed as described in the materials and methods. (C) Statistical analysis using 2 way ANOVA indicate highly significant effects for both time post-TNFα exposure and infection. Time and infection do not exhibit significant interaction. Significance: p values of 0.01 and under are considered highly significant (***), p values between 0.01 and 0.05 are considered significant (**) while those above 0.05 are not significant (ns).