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. Author manuscript; available in PMC: 2009 Dec 10.
Published in final edited form as: Exp Cell Res. 2008 Oct 7;314(20):3724–3736. doi: 10.1016/j.yexcr.2008.09.019

Fig. 3. Phospho-JNK levels are not affected by T. gondii infection in wild type mouse embryonic fibroblasts and p65−/− cells following UV exposure.

Fig. 3

Fig. 3

(A) A representative immunoblot of uninfected and T. gondii-infected wild type mouse embryonic fibroblasts following exposure to UV irradiation and harvested at the time points indicated. JNK-phosphorylation (PJNK) occurs within 15 minutes post UV and is sustained for the duration of the experiment in uninfected MEF cells similar to the pattern observed in HeLa cells. This response is fundamentally identical with regard to the extent of JNK phosphorylation and its kinetics in cells infected with T. gondii. Total JNK (JNK) and calnexin serve as loading controls. (B) Densitometric of phospho-p54 JNK levels relative to total JNK in six independent experiments depicts the range of values inherent in such experiments. Relative phospho-p54 levels in uninfected cells (circle) versus infected cells (diamond) confirm an absence of inhibition of mean phospho-p54JNK accumulation (horizontal line) in parasite-infected cells relative (diamond) to uninfected cells (circle). Densitometric analysis was performed as described in the materials and methods (C) Statistical analysis using two-way ANOVA indicate a highly significant effect for time post-UV but not for infection. Time and infection do not exhibit significant interaction. The identical experiment to that performed with wild type MEF was replicated in p65−/− fibroblasts. Results indicate the absence of any significant inhibition in relative phospho-p54 JNK levels. (E) Densitometric analysis of six independent experiments confirms the broad range of responses and the lack of apparent differences in the mean levels of phospho-p54 JNK comparing uninfected (circle) and infected (diamond) p65−/− cells. (F) Two way ANOVA analysis reveals a highly significant effect for time post-UV but not infection. Significance: p values of 0.01 and lower are considered highly significant (***), p values between 0.01 and 0.05 are considered significant (**) while those of above 0.05 are not significant (ns).