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. 1983 May;40(2):501–505. doi: 10.1128/iai.40.2.501-505.1983

Streptococcal growth and toxin production in vivo.

J L Duncan
PMCID: PMC264883  PMID: 6840849

Abstract

Streptococcal growth in vivo was studied with inoculated micropore filter chambers which were implanted into the peritoneal cavities of mice. Eight of nine group A strains and one group B strain grew in vivo, achieving concentrations of 10(7) to 10(9) CFU/ml in the chambers. Experiments with the Richards strain showed that the number of viable organisms remained high at 5 and 8 weeks after infection. The use of specific inhibitors and appropriate toxin-negative strains demonstrated that both cytolytic toxins produced by group A streptococci, streptolysin S and streptolysin O, were present in the culture fluids harvested from the chambers. This finding represents the first evidence that streptolysin S is produced in vivo. The host response to streptococci growing in vivo was examined by following the increase in serum antistreptolysin O levels. The response was first detected 2 weeks after chamber implantation and appeared to be maximal after 5 weeks. In addition, the production of antibody to streptococcal cell surface antigens was demonstrated indirectly with fluorescein-labeled anti-mouse immunoglobulin G.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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